Journals / International Journal of Secondary Metabolite / 2018 / Cilt: 5 - Sayı: 1

Biodiversity of Bacteria Isolated from Home-Made Wine and Vinegar

Pages
41–48
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to molecular results acetic acid and lactic acid bacteria were found.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Wine is an alcoholic beverage made grapes fermented without the addition of sugars, acids, enzymes, water.
📊
Key Findings & Data:For molecular identification 16S rDNA-PCR method was used.
💡
Conclusion & Impact:According to molecular results acetic acid and lactic acid bacteria were found.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:16S rDNA · Biodiversity · Wine

Abstract

Wine is an alcoholic beverage made grapes fermented without the addition of sugars, acids, enzymes, water. It has been consumed by human beings in religious ceremonies since ancient times. Vinegar is sour juice that is used as a sweetener in meals, in salads, or as a preservative such as brine. It has a great variety of industrial, medical, and domestic uses are still commonly practiced today. The aim of this study was to determine the bacterial biodiversity of home-made wine and vinegar using classic and molecular methods. Morphological, cultural and biochemical identifications were made according to the Bergey’s Manual of Systematic Bacteriology. For molecular identification 16S rDNA-PCR method was used. PCR results of these samples were send to the sequencing. BLASTn software was used to match our sequences with the ones in GenBank. In this study, bacteria colonies were isolated from home-made wine and vinegar. According to molecular results acetic acid and lactic acid bacteria were found.

Özet

Wine is an alcoholic beverage made grapes fermented without the addition of sugars, acids, enzymes, water. It has been consumed by human beings in religious ceremonies since ancient times. Vinegar is sour juice that is used as a sweetener in meals, in salads, or as a preservative such as brine. It has a great variety of industrial, medical, and domestic uses are still commonly practiced today. The aim of this study was to determine the bacterial biodiversity of home-made wine and vinegar using classic and molecular methods. Morphological, cultural and biochemical identifications were made according to the Bergey’s Manual of Systematic Bacteriology. For molecular identification 16S rDNA-PCR method was used. PCR results of these samples were send to the sequencing. BLASTn software was used to match our sequences with the ones in GenBank. In this study, bacteria colonies were isolated from home-made wine and vinegar. According to molecular results acetic acid and lactic acid bacteria were found.

🔖

Cite This Article

TÖRÜN, B., BIYIK, H. H., TOURAY, M., ÇOBAN, E. P. (2022). Biodiversity of Bacteria Isolated from Home-Made Wine and Vinegar. International Journal of Secondary Metabolite, Cilt: 5 - Sayı: 1, 41–48. https://ajindex.com/articles/651640

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Hainzelin, É., Nouaille, C. (2013). The Diversity of Living Organisms: The Engine for Ecological Functioning, É. Hainzelin (Eds.), Cultivating Biodiversity to Transform Agriculture, Montpellier, France, pp. 11-35.
  2. Johnston, C.S., Gaas, C.A. (2006). Vinegar: Medicinal Uses and Antiglycemic Effect. Medscape General Medicine, 8(2): 61-73.
  3. Liu, D.R., Zhu, Y., Beeftink, R., Ooijkaas, L., Rinzema, A., Chen, J. (2004). Chinese vinegar and its solid-state fermentation process. Food Reviews International, 20(4): 407- 424. Kersters, K., Lisdiyanti, P., Komagata, K., Swings, J. (2006). The family Acetobacteraceae: the genera Acetobacter, Acidomonas, Asaia, Gluconacetobacter, Gluconobacter, and Kozakia. Dworkin M., Falkow S., Rosenberg E., Schleifer K.H., Stackebrandt E. (Eds.), The Prokaryotes, Springer, New York, pp. 163-200.
  4. Pretorius, I.S., Van der Westhuizen, T.J., Augustyn, O.P.H. (1999). Yeast Biodiversity in vineyards and wineries and its importance to the South African Wine Industry. A Review. South African Journal for Enology and Viticulture, 20(2): 61-70.
  5. Wang, C.Y., Zhang, J., Gui, Z.Z. (2015). Acetobacter bacteria are found in Zhenjiang vinegar grains. Genetics and Molecular Research, 14(2): 5054-5064.
  6. Sharafi, S.M., Rasooli, I., Beheshti-Maal, K. (2010). Isolation, characterization and optimization of indigenous acetic acid bacteria and evaluation of their preservation methods. Iranian Journal of Microbiology, 2(1): 38-41.
  7. Krieg, N.R., Holt, J.G., (1984). Bergey’s Manual of Systematic Bacteriology. Williams and Wilkins, Baltimore, MD. De Boer, S.H., Ward, L.J. (1995). PCR detection of Erwinia carotovora subsp atroseptica associated with potato tissue. Phytopathology, 85(8): 854-858.
  8. Ruiz, P., Seseña, S., Izquierdo, P.M., Palop, M.L. (2010). Bacterial biodiversity and dynamics during malolactic fermentation of Tempranillo wines as determined by a culture-independent method (PCR-DGGE). Applied Microbiology and Biotechnology, 86(5): 1555-1562.
  9. Torija, M.J., Mateo, E., Guillamón, J.M., Mas, A. (2010). Identification and quantification of acetic acid bacteria in wine and vinegar by TaqMan–MGB probes. Food Microbiology, 27(2): 257-265.
  10. Sharafi, S.M., Rasooli, I., Beheshti-Maal, K. (2010). Isolation, characterization and optimization of indigenous acetic acid bacteria and evaluation of their preservation methods. Iranian Journal of Microbiology, 2(1): 38-45.
  11. Wu, J., Gullo, M., Chen, F., Giudici, P. (2010). Diversity of Acetobacter pasteurianus strains isolated