SavedAdd your journal

Journals / Anadolu Kardiyoloji Dergisi / 2011 / Cilt: 11 - Sayı: 2

Neointimal hiperplazi üzerine zoledronik asidin etkileri: Tavşan karotis anastomoz modeli

The effects of zoledronic acid on neointimal hyperplasia: a rabbit carotid anastomosis model - Original Investigation

Buket REEL
Mehmet GÜL
Eyüp HAZAN
Mehmet GÜZELOĞLU
Koray AYKUT
İsmail YÜREKLİ
Pages
93–100
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results: In PBS group, vascular injury in the anastomosed artery significantly increased the intimal area (anastomosed: 112.51±61.18 µm2*1000 vs. control: 22.62±4.26µm2*1000, p

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: The aim of the present study was to investigate the effect of zoledronic acid (ZA), as a matrix metalloproteinase inhibitor, on neointimal hyperplasia in rabbit carotid anastomosis model.
📊
Key Findings & Data:After anesthesia, the right carotid artery of each rabbit was end-to-end anastomosed with an 8/0 polypropylene suture.
💡
Conclusion & Impact:Results: In PBS group, vascular injury in the anastomosed artery significantly increased the intimal area (anastomosed: 112.51±61.18 µm2*1000 vs. control: 22.62±4.26µm2*1000, p
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of the present study was to investigate the effect of zoledronic acid (ZA), as a matrix metalloproteinase inhibitor, on neointimal hyperplasia in rabbit carotid anastomosis model. Methods: New Zealand male rabbits were divided into two groups as placebo and treatment groups in this experimental study. After anesthesia, the right carotid artery of each rabbit was end-to-end anastomosed with an 8/0 polypropylene suture. Left carotid artery was kept as control without any operation. Placebo group (n=6) received phosphate buffered saline (PBS) (0.5mL/kg/day/s.c.) for 28 days postoperatively, whereas ZA group (n=6) received ZA (100 µg/kg/day/s.c.) for the same period. After sacrification, the anastomosed and control arteries were isolated. Morphometric and immunohistochemical examinations were performed. Statistical analyses of morphometric and immunohistochemical data were performed using two-way ANOVA and Chi-square test respectively. Results: In PBS group, vascular injury in the anastomosed artery significantly increased the intimal area (anastomosed: 112.51±61.18 µm2*1000 vs. control: 22.62±4.26µm2*1000, p

Özet

Amaç: Çalışmamızın amacı tavşan karotis arter anastomoz modelinde gelişen neointimal hiperplazi üzerine bir matriks metalloproteinaz inhibitörü olarak zoledronik asidin (ZA) etkilerini araştırmaktır. Yöntemler: Bu deneysel çalışmada Yeni Zelanda tipi erkek tavşanlar plasebo ve ilaç-tedavi olmak üzere iki gruba ayrılmıştır. Anestezi sonrası, her bir tavşanın sağ karotis arteri 8/0 polipropilen sütür ile uç uca anastomoz edilmiştir. Sol karotis arter ise uygulama olmaksızın kontrol olarak bırakılmıştır. Plasebo grubundaki tavşanlara (n=6) operasyon sonrası 28 gün boyunca PBS (0.5mL/kg/gün/s.k.) uygulanırken ilaç grubundaki tavşanlara (n=6) aynı süre boyunca zoledronik asid (100 µg/kg/gün/s.k.) uygulanmıştır. Sakrifikasyondan sonra anastomozlu ve kontrol arterler izole edilmiştir. Morfometrik ve immünohistokimyasal incelemeler gerçekleştirilmiştir. Morfometrik ve immünohistokimyasal verilerin istatistiksel analizleri sırasıyla çift yönlü ANOVA ve Ki-kare testi ile yapılmıştır. Bulgular: PBS grubunda anastomozlu arterlerde oluşan damar hasarının, kontrol artere kıyasla intimal alanı (kontrol: 22.62±4.26, µm2*1000 anastomozlu: 112.51±61.18 µm2*1000, p

🔖

Cite This Article

REEL, B., GÜL, M., HAZAN, E., GÜZELOĞLU, M., AYKUT, K., YÜREKLİ, İ. (2022). Neointimal hiperplazi üzerine zoledronik asidin etkileri: Tavşan karotis anastomoz modeli. Anadolu Kardiyoloji Dergisi, Cilt: 11 - Sayı: 2, 93–100. https://ajindex.com/articles/648446

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Donaldson MC, Mannick JA, Whittemore AD. Causes of primary graft failure after in situ saphenous vein bypass grafting. J Vasc Surg 1992; 15: 113-8
  2. Merzkirch C, Davies N, Zilla P. Engineering of vascular in-growth matrices: are protein domains an alternative to peptides? Anat Rec 2001; 263: 379-87
  3. Mills JL. Mechanisms of vein graft failure: the location, distribution, and characteristics of lesions that predispose to graft failure. Semin Vasc Surg 1993; 6: 78-91
  4. De Meyer GR, Bult H. Mechanisms of neointima formation-lessons from experimental models. Vasc Med 1997; 2: 179-89
  5. Kuzuya M, Iguchi A. Role of matrix metalloproteinases in vascular remodeling. J Atheroscler Thromb 2003; 10: 275-82
  6. Garcia-Touchard A, Henry TD, Sangiorgi G, Spagnoli LG, Mauriello A, Conover C, et al. Extracellular proteases in atherosclerosis and restenosis. Arterioscler Thromb Vasc Biol 2005; 25: 1119-27
  7. Zempo N, Koyama N, Kenagy RD, Lea HJ, Clowes AW. Regulation of vascular smooth muscle cell migration and proliferation in vitro and in injured rat arteries by a synthetic matrix metalloproteinase inhibitor. Arterioscler Thromb Vasc Biol 1996; 16: 28-33
  8. Zaltsman AB, George SJ, Newby AC. Increased secretion of tissue inhibitors of metalloproteinases 1 and 2 from the aortas of cholesterol fed rabbits partially counterbalances increased metalloproteinase activity. Arterioscler Thromb Vasc Biol 1999; 19: 1700-7
  9. Godin D, Ivan E, Johnson C, Magid R, Galis ZS. Remodeling of carotid artery is associated with increased expression of matrix metalloproteinases in mouse blood flow cessation model. Circulation 2000; 102: 2861-6
  10. Baker AH, Edwards DR, Murphy G. Metalloproteinase inhibitors: biological actions and therapeutic opportunities. J Cell Sci 2002; 115: 3719-27
  11. Bendeck MP, Conte M, Zhang M, Nili N, Strauss BH, Farwell SM. Doxycycline modulates smooth muscle cell growth, migration, and matrix remodeling after arterial injury. Am J Pathol 2002; 160: 1089-95
  12. Li C, Cantor WJ, Nili N, Robinson R, Fenkell L, Tran YL, et al. Arterial repair after stenting and the effects of GM6001, a matrix metalloproteinase inhibitor. J Am Coll Cardiol 2002; 39: 1852-8
  13. Margolin L, Fishbein I, Banai S, Golomb G, Reich R, Perez LS, et al. Metalloproteinase inhibitor attenuates neointima formation and constrictive remodeling after angioplasty in rats: augmentative effect of alpha (v) beta (3) receptor blockade. Atherosclerosis 2002;163:269-77
  14. Catterall JB, Ca