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Journals / Türk Oftalmoloji Dergisi / 2014 / Cilt: 44 - Sayı: 5

İnsan kaynaklı doku yapıştırıcısı: İn Vitro antibakteriyel etkinliği ve antibiyotik geçirgenliği

Human-Derived Fibrin Glue: In Vitro antibacterial effects and antibiotic permeation

Erdem YÜKSEL
Nilay YÜKSEL
Fikret AKATA
Hasan Ali TUFAN
MERT DİNÇ. Bedia
Pages
347–350
Publication Date
2022-11-05
DOI
—
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Conclusion: Although FG has no antibacterial efficacy in vitro, it may be used safely due to antibiotic permeation in diseases with either infected or non-infected ocular surface that require suturing. (Turk J Ophthalmol 2014; 44: 347-50)

📌 3 Key Takeaways

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Objective & Hypothesis:Summary Objectives: This study investigated the in vitro antibacterial efficacy and antibiotic permeation of human-derived fibrin glue (FG).
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Key Findings & Data:In this study, cultivations were performed in 5 main groups: Group 1 (control) - only FG; Group 2a (control) - pure Staphylococcus aureus (SA) and Group 2b (control) - pure Staphylococcus epidermidis (SE); Group 3a (control) - SA+antibiotic and Group 3b (control) - SE+antibiotic; Group 4a - FG+SA and Group 4b - FG+SE; Group 5a - FG+SA+antibiotic and Group 5b - FG+SA+antibiotic.
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Conclusion & Impact:Conclusion: Although FG has no antibacterial efficacy in vitro, it may be used safely due to antibiotic permeation in diseases with either infected or non-infected ocular surface that require suturing. (Turk J Ophthalmol 2014; 44: 347-50)
🔬Design:Research Article
📍Scope:2014 Verileri
⚙️Variables:Domain Parameters

Abstract

Summary Objectives: This study investigated the in vitro antibacterial efficacy and antibiotic permeation of human-derived fibrin glue (FG). Materials and Methods: FG was prepared under sterile conditions by the Blood Bank of Gazi University Faculty of Medicine. In this study, cultivations were performed in 5 main groups: Group 1 (control) - only FG; Group 2a (control) - pure Staphylococcus aureus (SA) and Group 2b (control) - pure Staphylococcus epidermidis (SE); Group 3a (control) - SA+antibiotic and Group 3b (control) - SE+antibiotic; Group 4a - FG+SA and Group 4b - FG+SE; Group 5a - FG+SA+antibiotic and Group 5b - FG+SA+antibiotic. Results: Group 1 showed no bacterial growth, whereas Group 2a and Group 2b, Group 4a and Group 4b showed bacterial growth. Group 5a and Group 5b showed no growth. Conclusion: Although FG has no antibacterial efficacy in vitro, it may be used safely due to antibiotic permeation in diseases with either infected or non-infected ocular surface that require suturing. (Turk J Ophthalmol 2014; 44: 347-50)

Özet

Amaç: İnsan kaynaklı doku yapıştırıcısının in vitro antibakteriyel etkinliğini ve antibiyotik geçirgenliğini araştırmak. Gereç ve Yöntem: Çalışmada, kan bankasında steril koşullar altında hazırlanan doku yapıştırıcısı (DY) kullanıldı ve hazırlanan DY besiyerlerine ekildi. Çalışma 5 gruba ayrıldı. Grup 1 (kontrol), sadece DY, Grup 2a (kontrol); sadece Staphylococcus aureus (SA) and Group 2b (kontrol); sadece Staphylococcus epidermidis (SE), Group 3a (kontrol); SA+antibiyotik and Group 3b (kontrol); SE+antibiyotik, Group 4a; FG+SA and Group 4b; FG+SE, Group 5a; FG+SA+antibiyotik and Group 5b; FG+SA+antibiyotik. Bulgular: Grup 1de bakteriyel üreme görülmezken, Grup 2a ve Grup 2b, Grup 4a ve Grup 4bde bakteriyel üreme görüldü. Grup 5a ve Grup 5bde üreme saptanmadı. Sonuç: DY, in vitro antibakteriyel etkinlik göstermezken, antibiyotik geçirgenliğinden dolayı, sütürasyon gerektiren enfekte veya enfekte olmayan oküler yüzeylerde güvenle kullanılabilir. (Turk J Ophthalmol 2014; 44: 347-50)

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Cite This Article

YÜKSEL, E., YÜKSEL, N., AKATA, F., TUFAN, H. A., Bedia, M. D. (2022). İnsan kaynaklı doku yapıştırıcısı: İn Vitro antibakteriyel etkinliği ve antibiyotik geçirgenliği. Türk Oftalmoloji Dergisi, Cilt: 44 - Sayı: 5, 347–350. https://ajindex.com/articles/638083

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References (14)

  1. Sharma A, Kaur R, Kumar S, et al. Fibrin glue versus N-butyl-2- cyanoacrylate in corneal perforations. Ophthalmology. 2003;110:291-8.
  2. Duchesne B, Tahi H, Galand A. Use of human fibrin glue and amniotic membrane transplant in corneal perforation. Cornea. 2001;20:230-2.
  3. Kolega J, Manabe M, Sun TT. Basement membrane heterogeneity and variotion in corneal epithelial differentiation. Differentiation. 1989;42:54-63.
  4. Kurpakus MA, Stock EL, Jones JC. The role of the basement membrane in differential expression of keratin proteins in epithelial cells. Dev Biol. 1992:50:243-55.
  5. Weiss JL, Williams P, Lindstrom RL, Doughman DJ. The use of tissue adhesive in corneal perforations. Ophthalmology. 1983;90:610-5.
  6. Wessels IF, McNeill JI. Applicator for cyanoacrylate tissue adhesive. Opthalmic Surg. 1989;20:211-4.
  7. Lagoutte FM, Gauthier L, Comte PR. A fibrin sealant for perforated and preperforated corneal ulcers. Br J Ophthalmol. 1989;73:757-61.
  8. Hick S, Demers PE, Brunette I, La C, Mabon M, Duchesne B. Amniotic membrane transplantation and fibrin glue in the management of corneal ulcers and perforations: A review of 33 cases. Cornea. 2005;24:369-77.
  9. Blumenkranz MS, Ohana E, Shaikh S, et al. Adjuvant methods in macular hole surgery: intraoperative plasma-thrombin mixture and postoperative fluid-gas exchange. Ophthalmic Surg Lasers. 2001;32:198-207.
  10. Batman C, Ozdamar Y, Aslan O, Sonmez K, Mutevelli S, Zilelioglu G. Tissue glue in sutureless vitreoretinal surgery for the treatment of wound leakage. Ophthalmic Surg Lasers Imaging. 2008;39:100-6.
  11. Mestr U, Zuche M, Rauber M. Astigmatism after phacoemulsification with posterior chamber lens implantation: small incision technique with fibrin adhesive for wound closure. J Cataract Refract Surg. 1993;19:616-9.
  12. Seligsohn A, Moster MR, Steinmann W, Fontanarosa J. Use of Tisseel fibrin sealant to manage bleb leaks and hypotony; case series. J Glaucoma. 2004;13:227. Marticorena J, Rodríguez-Ares MT, Touriño R, et al. Pterygium surgery: conjunctival autograft using a fibrin adhesive. Cornea. 2006;25:34-3. ekiyama E, Nakamura T, Kurihara E, et al. Novel sutureless transplantation of bioadhesive-coated, freeze-dried amniotic membrane for ocular surface reconstruction. Invest Ophthalmol Vis Sci. 2007;48:1528-34.
  13. Pfister RR, Sommers CL. Fibrin sealant in corneal stem cell transplantation. Cornea 2005;24:593-8.
  14. Zagorski Z, Grunwald W, Naumann GO. Fibrin glue improves wound healing of non-perforating keratotomy. Fortschr Ophthalmol. 1989;86:581-