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Journals / Türk Biyokimya Dergisi / 2000 / Cilt: 25 - Sayı: 2

Karbontetraklorürün neden olduğu karaciğer sirozunda oksidan ve antioksidan parametrelerin değişimi

The changes of oxidative and antioxidative parameters in liver cirrhosis induced by carbon tetrachloride

İhsan HALİFEOĞLU
Fatma İNANÇ
Necip İLHAN
Nevin İLHAN
Pages
49–53
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Tissue MDA levels was significantly higher in the experimentally-induced cirrhosis group when compared to control group (p

📌 3 Key Takeaways

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Objective & Hypothesis:Cirrhosis is characterized with increased fibrosis and presence of regeneration nodules in liver.
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Key Findings & Data:Free radicals have been implicated in the pathogenesis of Carbon Tetrachloride (CCl4)-induced liver injury in rats.
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Conclusion & Impact:Tissue MDA levels was significantly higher in the experimentally-induced cirrhosis group when compared to control group (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Cirrhosis is characterized with increased fibrosis and presence of regeneration nodules in liver. Invitro and invivo studies indicate that oxidant stress is implicated in liver fibrogenesis. Free radicals have been implicated in the pathogenesis of Carbon Tetrachloride (CCl4)-induced liver injury in rats. Free radicals can cause oxidative damage to cellular proteins and alter cellular function. In the present study, we aimed to determine the effects of experimentally-induced liver cirrhosis on the serum Total Antioxidant Status (TAOS), tissue malondialdehyde (MDA), Sodium-Potassium Adenosine Triphosphatase (Na+-K+ATPase), and reduced Glutathione (GSH) levels in the rats. Two group of rats were randomized for this study; an experimental (n:20) and a control group (n:17). CCl4, was dissolved in olive oil (3/4; v/v) and subcutaneously administered to the rats for six weeks in order to induce cirrhosis. Controls received olive oil only. At the end of the study both rats in experimental and control groups were sacrificed. A part of the liver in each rat was removed for histopathological examination. Tissue MDA levels was significantly higher in the experimentally-induced cirrhosis group when compared to control group (p

Özet

Siroz; karaciğerde rejenerasyon nodüllerinin varlığı ve fıbrozisdeki artış ile tanımlanan bir hastalıktır. İnvitro ve invivo çalışmalar karaciğer fibrogenezinde oksidatif stresin önemli olduğunu göstermiştir. Katlarda karbon tetraklorür (CC14) ile oluşan karaciğer hasarının patogenezinde serbest radikallerin rolü önemlidir. Serbest radikaller hücre proteinlerini oksidatif hasara uğratır ve hücrelerin fonksiyonunu değiştirir. Çalışmada rotlarda deneysel olarak oluşturulmuş karaciğer sirozunun, serum total antioksidan status (TAOS), doku malondialdehid (MDA), doku sodyum potasyum adenozin trifosfataz (Na+-K+ ATPaz) ve redükte glutatyon (GSH) düzeyleri üzerine etkilerinin incelenmesi amaçlanmıştır. Çalışmada 20 adet rat'dan oluşan bir deney grubu ile 17 adet rat'dan oluşan bir kontrol grubu oluşturuldu. Siroz oluşturmak için CC14 zeytin yağı içinde 3/4 (v/v) oranında karıştırılarak 6 hafta süre ile deri altına uygulandı. Kontrol grubuna ise sadece zeytin yağı uygulandı. Uygulamayı takiben hem kontrol grubu hem de deney grubundaki ratlar dekapite edildi. Rafların karaciğerinin bir kısmı histopatolojik inceleme için ayrıldı. Doku MDA düzeyleri, siroz oluşturulmuş grupta kontrol grubu ile karşılaştırıldığında anlamlı olarak yüksek bulundu (p

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Cite This Article

HALİFEOĞLU, İ., İNANÇ, F., İLHAN, N., İLHAN, N. (2022). Karbontetraklorürün neden olduğu karaciğer sirozunda oksidan ve antioksidan parametrelerin değişimi. Türk Biyokimya Dergisi, Cilt: 25 - Sayı: 2, 49–53. https://ajindex.com/articles/621466

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