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Journals / Türk Biyokimya Dergisi / 2014 / Cilt: 39 - Sayı: 2

Screening of phytate hydrolysis Bacillus sp. isolated DEDE from soil and optimization of the certain nutritional and physical parameters on the production of phytase

Topraktan fitat hidrolize eden Bacillus sp. ‘lerin taranması ve fitaz üretimi üzerine bazı besinsel ve fiziksel faktörlerin optimizasyonu

Elif DEMİRKAN
Alev USTA
Eren BAYGIN
Pages
206–214
Publication Date
2022-11-05
DOI
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⚡ TL;DR(Too Long; Didn't Read)

In this medium, enzyme yield was enhanced 62% compared to basal medium.Conclusion: The present study suggests that the novel Bacillus sp. phytase enzyme may have wide industrial application, and can be used as an animal feed additive

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: To isolate phytase producing Bacillus sp. from soil samples of Turkey, and optimize the growth conditions for maximum production of phytase.Material and Methods: The screening of isolates was performed on phytase screening medium.
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Key Findings & Data:Phytase activity was determined by measur ing the amount of liberated inorganic phosphate.
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Conclusion & Impact:In this medium, enzyme yield was enhanced 62% compared to basal medium.Conclusion: The present study suggests that the novel Bacillus sp. phytase enzyme may have wide industrial application, and can be used as an animal feed additive
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: To isolate phytase producing Bacillus sp. from soil samples of Turkey, and optimize the growth conditions for maximum production of phytase.Material and Methods: The screening of isolates was performed on phytase screening medium. The best producer was selected. Phytase activity was determined by measur ing the amount of liberated inorganic phosphate. Optimal culture conditions and fermentation parameters for phytase production were assessed.Results: 236 Bacillus sp. strains isolated. The best phytase producing strain showed higher enzyme yield in the presence of wheat bran and lactose as carbon source, meat extract as organic nitrogen source, CaCl2 as metal source. 0.3% as phytate concentration was found to be best. In the physical parameters, the best results was obtained at 35°C, pH 7.5, 200 rpm as agitation rate, 2-4% as inoculum size and 48 h as inoculum age. A new medium was obtained by optimizing the incubation conditions of phytase production from Bacillus sp. EBD 9-1. In this medium, enzyme yield was enhanced 62% compared to basal medium.Conclusion: The present study suggests that the novel Bacillus sp. phytase enzyme may have wide industrial application, and can be used as an animal feed additive

Özet

Amaç: Türkiye'nin toprak örneklerinden fitaz üreten Bacillus sp. 'lerin izole ve fitazın maksimum üretimi için üreme şartlarının optimize edilmesidir.Gereç ve Yöntem: İzolatların taranması fitaz tarama ortamında gerçekleştirilmiştir. En iyi üretici seçilmiştir. Fitaz aktivitesi ortaya salınan inorganik fosfatın miktarı ölçülerek belirlenmiştir. En iyi kültür koşulları ve fermantasyon parametreleri fitaz üretimi için değerlendirilmiştir.Bulgular: 236 Bacillus sp. suşu izole edilmiştir. En iyi fitaz üreten suş yüksek enzim verimini karbon kaynağı olarak buğday kepeği ve laktoz, organik azot kaynağı olarak meat ekstrakt, metal kaynağı olarak CaCl2 varlığında göstermiştir.% 0.3 fitat konsantrasyonu en iyi olarak bulunmuştur. Fiziksel parametreler içinde, en iyi sonuçlar 35°C'de, pH 7.5'de, çalkalama oranı olarak 200 rpm'de, inokülüm miktarı olarak % 2-4 'de ve inokülüm yaşı olarak 48 saat'de elde edilmiştir. Bacillus sp. EBD 9-1'den fitaz üretimin inkübasyon şartlarının optimize edilmesiyle yeni bir ortam elde edilmiştir. Bu ortamda, enzim verimi temel ortamla karşılaştırıldığında % 62 artmıştır.Sonuç: Mevcut çalışma yeni Bacillus sp. fitaz enziminin geniş endüstriyel uygulamaya sahip olabilirliğini ve hayvan yemi katkı maddesi olarak kullanılabilirliğini önermektedir

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Cite This Article

DEMİRKAN, E., USTA, A., BAYGIN, E. (2022). Screening of phytate hydrolysis Bacillus sp. isolated DEDE from soil and optimization of the certain nutritional and physical parameters on the production of phytase. Türk Biyokimya Dergisi, Cilt: 39 - Sayı: 2, 206–214. https://ajindex.com/articles/621392

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