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Journals / Türk Biyokimya Dergisi / 2014 / Cilt: 39 - Sayı: 4

Effect of adipose tissue-derived inflammatory and proangiogenic cytokines on proliferative diabetic retinopathy

[adipoz doku kaynaklı inflamatuvar ve proanjiyojenik sitokinlerin proliferatif diyabetik retinopati üzerine etkisi]

Sibel KADAYIFÇILAR
Sevilay KARAHAN
Demet ABAN YABANOĞLU
Çağman SUN TAN
Bora ELDEM
Pages
495–502
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However, obesity may not be the root of the inflammatory mediators in PDR.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: To determine the vitreous and serum concentrations of TNF-?, IL-6, VEGF, IL-1?, IL-8, IL-17, MCP-1, IL-1Ra, IL-10 in patients with proliferative diabetic retinopathy (PDR) and to investigate the effect of adipose tissue on the pathogenesis of PDR.Methods: Twenty-two patients with PDR were prospectively evaluated.
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Key Findings & Data:Seven cadavers and 11 patients with idiopathic epiretinal membrane or macular hole served as the controls.
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Conclusion & Impact:However, obesity may not be the root of the inflammatory mediators in PDR.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: To determine the vitreous and serum concentrations of TNF-?, IL-6, VEGF, IL-1?, IL-8, IL-17, MCP-1, IL-1Ra, IL-10 in patients with proliferative diabetic retinopathy (PDR) and to investigate the effect of adipose tissue on the pathogenesis of PDR.Methods: Twenty-two patients with PDR were prospectively evaluated. Seven cadavers and 11 patients with idiopathic epiretinal membrane or macular hole served as the controls. Multiplex bead array technology was employed to assess the concentrations of the cytokines.Results: The intravitreal levels of VEGF, IL-8, IL-6 were significantly higher in PDR group than control groups. In PDR group, the levels of IL-17, IL-8, IL-6 were significantly elevated in the vitreous, whereas the intravitreal levels of IL-10, IL-1Ra were found to be significantly lower than the serum concentrations. No significant correlation was found between cytokine levels and body mass index (BMI), fasting blood glucose (FBG), or glycated haemoglobin (HbA1c) of diabetic patients.Conclusion: In PDR the balance between the intravitreal pro- and anti- inflammatory adipocytokines is disturbed in favor of proinflammatory and proangiogenic cytokines in the vitreous humour. This study supports the role of adipocytokines in vascular pathology in PDR. It seems that PDR is a local inflammatory disease. However, obesity may not be the root of the inflammatory mediators in PDR.

Özet

Amaç: Proliferatif diyabetik retinopati'de (PDR) serum ve vitreus TNF-?, IL-6, VEGF, IL-1?, IL-8, IL-17, MCP-1, IL-1Ra, IL-10 konsantrasyonlarını saptamak ve adipoz doku kaynaklı inflamatuvar sitokinlerin PDR patogenezindeki etkisini araştırmaktır.Metod: Yirmi iki PDR hastası prospektif olarak değerlendirildi. Kontrol grubu olarak 7 kadavra ile 11 idiyopatik epiretinal membran veya maküler delik olgusu değerlendirildi. Adipositokin konsantrasyonlarını değerlendirmede çoklu boncuk analizi yöntemi kullanıldı.Bulgular: Vitreus VEGF, IL-6 ve IL-8 konsantrasyonları PDR grubunda kontrol gruplarına göre anlamlı olarak yüksek saptandı. PDR grubunda vitreus IL-17, IL-6 ve IL-8 konsantrasyonları serum değerlerinden anlamlı olarak yüksek saptanırken, vitreus IL-10 ve IL-1Ra değerleri serum değerlerinden anlamlı olarak düşük bulunmuştur şeklinde olacaktır. Diyabetik hastalarda vücut kütle indeksi, açlık kan glukozu ve glikozile hemoglobin ile adipositokin konsantrasyonları arasında istatistiksel olarak anlamlı bir korelasyon saptanmamıştır.Sonuç: PDR'de vitreus pro- ve anti- inflamatuvar adipositokinler arasındaki denge proinflamatuvar ve proanjiyojenik sitokinler lehine bozulmuştur. Çalışmamız PDR'deki damarsal patolojide adipositokinlerin rolünü desteklemektedir. Öyle görünüyor ki PDR lokalize bir inflamatuvar hastalıktır. Bununla birlikte obezite PDR'de inflamatuvar moleküllerin asıl kaynağı olmayabilir.

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Cite This Article

KADAYIFÇILAR, S., KARAHAN, S., YABANOĞLU, D. A., TAN, Ç. S., ELDEM, B. (2022). Effect of adipose tissue-derived inflammatory and proangiogenic cytokines on proliferative diabetic retinopathy. Türk Biyokimya Dergisi, Cilt: 39 - Sayı: 4, 495–502. https://ajindex.com/articles/621220

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