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Journals / Türk Biyokimya Dergisi / 2016 / Cilt: 41 - Sayı: 5

Topraktan izole edilen bakterilerden biyosurfaktan üretiminin optimizasyonu ve surfaktanın karakterizasyonu

Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant

Pınar Aytar ÇELİK
Yeliz Buruk ŞAHİN
Meltem ÇELİKDEMİR
Nimetullah BURNAK
Yağmur TOPTAŞ
Vural BÜTÜN
Cansel TUNCER
Ahmet ÇABUK
Pages
338–346
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: This study reported that Georgenia daeguensis is able to produce effective biosurfactant exhibiting antibacterial activity against K. pneumoniae.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Biosurfactants are surface-active substances produced by microorganisms.
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Key Findings & Data:In this study, the capacity of biosurfactant production of Georgenia daeguensis (accession number, KP798810) isolated from hydrocarbon-contaminated soil was studied.Methods: The components of biosurfactant production medium were investigated through Plackett-Burman methodology.
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Conclusion & Impact:Conclusion: This study reported that Georgenia daeguensis is able to produce effective biosurfactant exhibiting antibacterial activity against K. pneumoniae.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Biosurfactants are surface-active substances produced by microorganisms. The growth of microorganism supports biosurfactant production on hydrocarbon polluted environments. In this study, the capacity of biosurfactant production of Georgenia daeguensis (accession number, KP798810) isolated from hydrocarbon-contaminated soil was studied.Methods: The components of biosurfactant production medium were investigated through Plackett-Burman methodology. By using Placket-Burman based on statistical screening, six of the eleven factors of production medium were found to be critically effective on the biosurfactant production.Results: The significant factors were KCl, ZnSO4.7H2O, CaCl2.2H2O, KH2PO4, MgSO4.7H2O and glucose. Among of these, KCl had the highest effect contribution with 55.22%. After the optimization by such design, critical micelle concentration value was reached to 2.2 g L-1. When anti bacterial activity of biosurfactant was screened, it showed antibacterial effect against to Klebsiella pneumoniae. Besides, the biosurfactant was characterized by FT-IR, critic micelle concentration and zeta analysis. Conclusion: This study reported that Georgenia daeguensis is able to produce effective biosurfactant exhibiting antibacterial activity against K. pneumoniae.

Özet

Amaç: Biyosurfaktanlar, mikroorganizmalar tarafından üretilen yüzey aktif bileşiklerdir. Hidrokarbonla kirlenmiş çevrelerde mikroorganizmanın gelişmesi biyosurfaktan üretimini destekler. Bu çalışmada hidrokarbonca kirlenmiş topraktan izole edilen Georgenia daeguensis (giriş numarası, KP798810)'nin biyosurfaktan üretme kapasitesi çalışılmıştır.Metod: Biyosurfaktan üretim ortamının bileşenleri Plackett-Burman metodolojisi kullanılarak değerlendirilmiştir. Istatistiksel taramaya bağlı Plackett-Burman kullanılarak, üretim ortamının 11 faktörden 6'sının biyosurfaktan üretiminde önemli ölçüde etkili olduğunu ortaya çıkarılmıştır. Bulgular: Önemli faktörler; KCl, ZnSO4 .7H2 O, CaCl2 .2H2 O, KH2 PO4 , MgSO4 .7H2 O ve glukoz olarak belirlenmiştir. Bunlarına arasından KCl, %55,2 ile en yüksek katkıya sahip olmuştur. Bu şekilde gerçekleştirilen optimizasyon sonrası, kritik misel konsantrasyonu 2,2 g L-1'ye ulaşmıştır. Biyosurfaktanın antibakteriyel aktivitesi değerlendirildi- ğinde Klebsiella pneumoniae'ye karşı antibakteriyel etkiye sahip olduğu gösterilmiştir. Bunun yanısıra, biyosurfaktan FTIR, kritik misel konsantrasyonu ve zeta analizi açı- sından da karakterize edilmiştir. Sonuç: Bu çalışma Georgenia daeguensis'in, K. pneumoniae'ye karşı antibakteriyel etki sergileyen etkili bir şekilde biyosurfaktan üretebildiğini bildirmektedir.

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Cite This Article

ÇELİK, P. A., ŞAHİN, Y. B., ÇELİKDEMİR, M., BURNAK, N., TOPTAŞ, Y., BÜTÜN, V., TUNCER, C., ÇABUK, A. (2022). Topraktan izole edilen bakterilerden biyosurfaktan üretiminin optimizasyonu ve surfaktanın karakterizasyonu. Türk Biyokimya Dergisi, Cilt: 41 - Sayı: 5, 338–346. https://ajindex.com/articles/621030

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