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Journals / Biyolojik Çeşitlilik ve Koruma / 2026 / Volume: 14 Issue: 2

Boron mine ponds: metagenomic insight to bacterial diversity

Bor maden göletleri: bakteriyel çeşitliliğe metagenomik bakış

Pınar Aytar Çelik(ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ, ESKİŞEHİR MESLEK YÜKSEKOKULU)0000-0002-9447-1668
Mehmet Burçin Mutlu(ESKİŞEHİR TEKNİK ÜNİVERSİTESİ, FEN FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ)0000-0002-9404-6389
Ferhan Korkmaz(ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ)0000-0002-3797-842X
Belma Nural Yaman(ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ, MÜHENDİSLİK-MİMARLIK FAKÜLTESİ)0000-0003-2576-1300
Serap Gedikli(ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ)0000-0001-5632-6585
Doç. Dr. Ahmet Çabuk(ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ)0000-0002-4619-6948
Pages
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Publication Date
2021-08-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Striking seasonal variations were detected between samples in terms of the type and number of microbial populations.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Since it can be a source of new microorganisms with biological potential, it is important to identify the microorganisms found in environments with high boron content in terms of ecological and biotechnological application potentials.
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Key Findings & Data:In this study, bacterial community in the waste mining ponds of Balıkesir-Bigadiç and Eskişehir-Kırka Boron Mining Operations were investigated by using 16S rDNA gene-targeted Illumina MiSeq sequencing.
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Conclusion & Impact:Striking seasonal variations were detected between samples in terms of the type and number of microbial populations.
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:boron · high throughput sequencing · microbial diversity

Abstract

Since it can be a source of new microorganisms with biological potential, it is important to identify the microorganisms found in environments with high boron content in terms of ecological and biotechnological application potentials. In this context, deposit waters in environments where boron mining activities perform are important habitats for boronophilic/boronotolerant microorganisms. In this study, bacterial community in the waste mining ponds of Balıkesir-Bigadiç and Eskişehir-Kırka Boron Mining Operations were investigated by using 16S rDNA gene-targeted Illumina MiSeq sequencing. The greater parts of high-throughput sequences were related to Proteobacteria, Planctomycetes, Bacteriodetes, Verrucomicrobia and Actinobacteria phyla. Cyanobacteria and Parcubacteria were other notable groups with low abundance. Genera belonged to Blastopirellula, Luteolibacter, Porhyrobacter and Hydrogenophaga were the most abundant taxa for all the samples. Sandarakinorhabdus, Pseudoanabena, Roseinatronobacter and Pontimonas genera-affiliated reads were also detected at in the October samples. Striking seasonal variations were detected between samples in terms of the type and number of microbial populations.

Özet

Biyolojik potansiyeli olan yeni mikroorganizmaların kaynağı olabileceği için, bor içeriği yüksek ortamlarda bulunan mikroorganizmaların belirlenmesi ekolojik ve biyoteknolojik uygulama potansiyelleri açısından önemlidir. Bu bağlamda, bor madenciliği faaliyetlerinin gerçekleştirildiği çevrelerdeki birikinti suları boronofilik / boronotolerant mikroorganizmalar için önemli habitatlardır. Bu çalışmada, Balıkesir-Bigadiç ve Eskişehir-Kırka Bor Maden İşletmeleri atık madenciliği havuzlarındaki bakteri topluluğu, 16S rDNA gen hedefli Illumina MiSeq dizilimi kullanılarak incelenmiştir. Yüksek verimli dizilerin büyük kısımları Proteobacteria, Planctomycetes, Bacteriodetes, Verrucomicrobia ve Actinobacteria filumları ile ilişkili bulunmuştur. Cyanobacteria ve Parcubacteria, düşük miktara sahip diğer önemli gruplar arasındadır. Blastopirellula, Luteolibacter, Porhyrobacter ve Hydrogenophaga'ya ait cinsler tüm örneklerde en çok bulunan taksonlardır. Ekim örneklerinde Sandarakinorhabdus, Pseudoanabena, Roseinatronobacter ve Pontimonas cinsine bağlı okumalar da tespit edilmiştir. Mikrobiyal popülasyonların türü ve sayısı açısından örnekler arasında çarpıcı mevsimsel farklılıklar tespit edilmiştir.

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Cite This Article

Çelik, P. A., Mutlu, M. B., Korkmaz, F., Yaman, B. N., Gedikli, S., Çabuk, D. D. A. (2021). Boron mine ponds: metagenomic insight to bacterial diversity. Biyolojik Çeşitlilik ve Koruma, Volume: 14 Issue: 2. https://doi.org/10.46309/biodicon.2021.902221

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