SavedAdd your journal

Journals / Türk Biyokimya Dergisi / 2018 / Cilt: 43 - Sayı: 2

Effects of chromium picolinate on the parameters of oxidative and chromosomal DNA damage in rabbits

Krom pikolinat’ın tavşanlarda oksidatif ve kromozomal DNA hasarı parametrelerine etkisi

Meryem ŞENTÜRK
Zuhal HAMURCU
Fatma UYANIK
Pages
110–118
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Slight or significant differenceswere determined in all investigated parametersbetween the measurements of on days 25 and 50.Conclusion: Improvements in of MDA and 8-OHdG levelsand genome damage indicators due to CrPic and picolinicacid on the 25th day of the treatment may show that shortterm of CrPic supplementation reduces oxidative andchromosomal DNA damage in rabbits.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objectives: This study investigated the effects of chromiumchloride (CrCl3 · 6H2O), chromium picolinate (CrPic)and picolinic acid on malondialdehyde (MDA), 8-hidroksi-2′-deoksiguanosine (8-OHdG) and genome damage inrabbits.Material and methods: Forty, New Zealand rabbits wereequally assigned to four groups which received eitherdistilled water or 20 mg/day Cr (CrC13 · 6H20), 200 μg/dayCrPic and 1400 μg/day picolinic acid for 50 days.
📊
Key Findings & Data:On the25th and 50th days, MDA and 8-OHdG levels and the apoptotic-necrotic cells, micronucleus (MN), micronuclearbuds (NBUD) and nucleoplasmic bridges (NPB) frequenciesand on the 50th day, serum Cr and liver MDA levelswere determined.Results: CrPic increased live weight and feed consumption.On the 25th day of treatment, decreases were detectedin MDA levels and MN, NPK and NBUD frequencies inCrPic and picolinic acid groups, and in 8-OHdG levelsin CrCl3 · 6H2O and CrPic groups.
💡
Conclusion & Impact:Slight or significant differenceswere determined in all investigated parametersbetween the measurements of on days 25 and 50.Conclusion: Improvements in of MDA and 8-OHdG levelsand genome damage indicators due to CrPic and picolinicacid on the 25th day of the treatment may show that shortterm of CrPic supplementation reduces oxidative andchromosomal DNA damage in rabbits.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: This study investigated the effects of chromiumchloride (CrCl3 · 6H2O), chromium picolinate (CrPic)and picolinic acid on malondialdehyde (MDA), 8-hidroksi-2′-deoksiguanosine (8-OHdG) and genome damage inrabbits.Material and methods: Forty, New Zealand rabbits wereequally assigned to four groups which received eitherdistilled water or 20 mg/day Cr (CrC13 · 6H20), 200 μg/dayCrPic and 1400 μg/day picolinic acid for 50 days. On the25th and 50th days, MDA and 8-OHdG levels and the apoptotic-necrotic cells, micronucleus (MN), micronuclearbuds (NBUD) and nucleoplasmic bridges (NPB) frequenciesand on the 50th day, serum Cr and liver MDA levelswere determined.Results: CrPic increased live weight and feed consumption.On the 25th day of treatment, decreases were detectedin MDA levels and MN, NPK and NBUD frequencies inCrPic and picolinic acid groups, and in 8-OHdG levelsin CrCl3 · 6H2O and CrPic groups. Slight or significant differenceswere determined in all investigated parametersbetween the measurements of on days 25 and 50.Conclusion: Improvements in of MDA and 8-OHdG levelsand genome damage indicators due to CrPic and picolinicacid on the 25th day of the treatment may show that shortterm of CrPic supplementation reduces oxidative andchromosomal DNA damage in rabbits.

Özet

Amaç: Bu çalışma, tavşanlarda krom klorür (CrCl3 · 6H2O), krom pikolinat (CrPic) ve pikolinik asidin malondialdehit (MDA), 8-hidroksi-2′-deoksiguanozin (8-OHdG) ve genom hasarı üzerine etkilerinin belirlenmesi amacıyla gerçekleştirildi. Gereç ve Yöntem: Çalışmada 40 Yeni Zelanda tavşanı, eşit sayıda olacak şekilde 4 gruba ayrılarak distile su veya CrCl3 · 6H2O formunda 20 mg/gün Cr, 200 μg/gün CrPic ve 1400 μg/gün pikolinik asit 50 gün verildi. Denemenin 25. ve 50. günlerinde MDA ve 8-OHdG düzeyleri ve apoptotik -nekrotik hücre sayıları, mikronükleus (MN), nükleoplazmik köprü (NPK) ve nükleer bud (NBUD) sıklığı ile denemenin 50. gününde serum Cr ve karaciğer MDA düzeyleri belirlendi. Bulgular: Chromium picolinate (CrPic) yem tüketimi ve canlı ağırlığı arttırdı. Çalışmanın 25. gününde, CrPic ve pikolinik asit verilen gruplarda MDA düzeyleri ile MN, NPK ve NBUD sıklığı, CrCl3 · 6H2O ve CrPic verilen gruplarda da 8-OHdG düzeyleri azaldı. İncelenen tüm parametreler yönünden 25. ve 50. günlerde yapılan ölçümler arasında önemsiz yada önemli farklılıklar saptandı. Sonuç: Çalışmanın 25. gününde CrPic ve pikolinik asit; MDA, 8-OHdG ve genom hasarı göstergelerinde iyileşmeye neden olduğundan tavşanlara kısa süreli CrPic verilmesinin oksidatif ve kromozomal DNA hasarının önlenmesinde etkili olabileceği kanaatine varıldı.

🔖

Cite This Article

ŞENTÜRK, M., HAMURCU, Z., UYANIK, F. (2022). Effects of chromium picolinate on the parameters of oxidative and chromosomal DNA damage in rabbits. Türk Biyokimya Dergisi, Cilt: 43 - Sayı: 2, 110–118. https://ajindex.com/articles/620979

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (16)

  1. Mossop RT. Effects of chromium (III) on fasting blood glucose, cholesterol and cholesterol HDL levels in diabetic. Central African J Med 1983;29:80–2
  2. Striffler JS, Law JS, Polansky MM, Bhathena SJ, Anderson RA. Chromium improves insulin response to glucose in rats. Metabolism 1995;44:1314–20
  3. Anderson RA, Roussel AM, Zouari N, Mahjoub S, Matheau JM, Kerkeni A. Potential antioxidant effects of zinc and chromium supplementation in people with type 2 diabetes mellitus. Am Coll Nutr 2001;20:212–8
  4. Ducros V. Chromium metabolism. A literature review. Biol Trace Elem Res 1992;32:65–77
  5. Chen SY, Lien TF. Toxicity evaluation of chromium picolinate nanoparticles in vivo and in vitro in rat. Biol Trace Elem Res 2013;151:247–55
  6. Prasad AS. Chromium. Trace elements and iron in human metabolism. New York: John Wiley & Sons LTD, 1978:3–12
  7. World Health Organisation. Chromium. Genova, 1996:155–9
  8. Sell JL. The role of chromium in animal nutrition. Washington: National Academy Press, 1997:80
  9. Lukaski HC, Siders WA, Penland JG. Chromium picolinate supplementation in women: effects on body weight, composition, and iron status. Nutr 2007;23:187–95
  10. Crawford V, Scheckenbach R, Preuss HG. Effects of niacin-bound chromium supplementation on body composition in overweight African-American women. Diabetes Obes Metab 1999;1:331–7
  11. İnanç N, Uyanık F, Şahin H, Yaman H, Erdem O. Effects of chromium supplementation on body composition, leptin, ghrelin levels and selected biochemical parameters in obese women. Trace Elem Electrol 2006;23:128–33
  12. Cupo MA, Donaldson WE. Chromium and vanadium effects on glucose metabolism and lipid synthesis in the chick. Poultry Sci 1987;66:120–6
  13. Lien TF, Horng YM, Yang KH. Performance, serumcharactesictics, carcase traits and lipid metabolism of broilers as affected by supplement of chromium picolinate. Br Poult Sci 1999;40: 357–63
  14. Uyanık F, Atasever A, Özdamar S, Aydın F. Effects of dietary chromium chloride supplementation on performance, some serum parameters and immune response in broilers. Biol Trace Elem Res 2002;90:99–115
  15. Eren M, Başpınar N. Effects of dietary CrCl3 supplementation on some serum biochemical markers in broilers, Influence of season, age and sex. Revue de Med Vet 2004;155:637–41
  16. Smith KL, Waldron MR, Drackley JK, Socha MT, Overton TR. Performance of dairy cows as affected by prepartum dietary carbohydrate source and supplementation with chromium throughout the transition per