SavedAdd your journal

Journals / Türk Biyokimya Dergisi / 2018 / Cilt: 43 - Sayı: 4

In vitro bioactivities and subacute toxicity study of O. basilicum, T. vulgaris and R. officinalis

O. basilicum, T. vulgaris ve R. officinalis’in in vitro biyolojik aktiviteleri ve subakut toksisite çalışması

Iram MURTAZA
Touseef REHAN
Riffat TAHIRA
Hanif ULLAH
Usman TAREEN
Tabassum REHAN
Mariam ANEES
Aneesa SULTAN
Pages
447–455
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Thymus vulgaris and O. basilicum extract significantlyincreased red blood cells, packed cell volume,hemoglobin and mean corpuscular volume at 1500 mg/kgbody weight.Conclusion: Ocimum basilicum, Thymus vulgaris and Rosmarinusofficinalis have good phenolics and flavonoidcontent that shows antioxidant and antibacterial potentialwhilst having no evident toxic side effects on mammaliantissue and hematological parameters.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background: Ocimum basilicum, Thymus vulgaris, andRosmarinus officinalis have been used for the treatmentof different ailments for a historically long time but therehave been no safety studies of these plants.
📊
Key Findings & Data:For toxicityassay, rats were treated with extracts of three plants at1000 and 1500 mg/kg body weight.Results: Ocimum basilicum extract showed highest totalphenols, flavonoids, antioxidant and antibacterial activities.Thymus vulgaris extract caused hypertrophy of liverwhile Rosmarinus officinalis caused atrophy of spleen atboth doses showing no significant histomorphologicalchanges.
💡
Conclusion & Impact:Thymus vulgaris and O. basilicum extract significantlyincreased red blood cells, packed cell volume,hemoglobin and mean corpuscular volume at 1500 mg/kgbody weight.Conclusion: Ocimum basilicum, Thymus vulgaris and Rosmarinusofficinalis have good phenolics and flavonoidcontent that shows antioxidant and antibacterial potentialwhilst having no evident toxic side effects on mammaliantissue and hematological parameters.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: Ocimum basilicum, Thymus vulgaris, andRosmarinus officinalis have been used for the treatmentof different ailments for a historically long time but therehave been no safety studies of these plants. Phytoconstituentsof these plants were found to be good potentialtherapeutic agents which could be used in treatment regimensas a replacement of synthetic drugs if they are safe.Experimental: Standard Folin ciocalteu reagent assay,aluminum chloride colorimetric assay and DPPH assaywere employed to determine total phenolic, flavonoidsand antioxidant activity, respectively. Broth microdilutionmethod was used for investigation of synergisticeffects of plant extracts with antibiotics. For toxicityassay, rats were treated with extracts of three plants at1000 and 1500 mg/kg body weight.Results: Ocimum basilicum extract showed highest totalphenols, flavonoids, antioxidant and antibacterial activities.Thymus vulgaris extract caused hypertrophy of liverwhile Rosmarinus officinalis caused atrophy of spleen atboth doses showing no significant histomorphologicalchanges. Thymus vulgaris and O. basilicum extract significantlyincreased red blood cells, packed cell volume,hemoglobin and mean corpuscular volume at 1500 mg/kgbody weight.Conclusion: Ocimum basilicum, Thymus vulgaris and Rosmarinusofficinalis have good phenolics and flavonoidcontent that shows antioxidant and antibacterial potentialwhilst having no evident toxic side effects on mammaliantissue and hematological parameters.

Özet

Amaç: O. basilicum, T. vulgaris ve R. officinalis, farklı hastalıkların tedavisinde uzun zamandır kullanılmaktadır ancak bu bitkilerin hiçbir güvenlik araştırması yapılmamıştır. Bu bitkilerin fito-konstitütiflerinin, tedavi rejimlerinde sentetik uyuşturucuların yerini alması halinde kullanılabilecek iyi potansiyel terapötik ajanlar olduğu tespit edildi. Deneysel: Sırasıyla toplam fenolik, flavonoidleri ve antioksidan aktiviteyi belirlemek için Standart Folin ciocalteu reaktif testi, alüminyum klorür kolorimetrik assay ve DPPH testi kullanıldı. Bitki özlerinin antibiyotik ile sinerjik etkilerinin araştırılması için et suyu mikrodilüsyon yöntemi kullanılmıştır. Toksisite tahlili için sıçanlar üç bitki ekstraktları ile 1000 ve 1500 mg/kg vücut ağırlığı ile muamele edildi. Bulgular: O. basilicum özütü, toplam fenol, flavonoid, antioksidan ve antibakteriyel aktivitelerde en yüksek oranda saptandı. T. vulgaris ekstraktı karaciğer hipertrofisine neden olurken, R. officinalis her iki dozda da dalak atrofisine neden olmuş, önemli bir histomorfolojik değişiklik göstermemiştir. T. vulgaris ve O. basilicum özü, 1500 mg/kg vücut ağırlığında kırmızı kan hücrelerini, paketlenmiş hücre hacmini, hemoglobin ve ortalama vücut hacmini önemli ölçüde arttırdı. Sonuç: O. basilicum, T. vulgaris ve R. officinalis, antioksidan ve antibakteriyel potansiyel gösteren iyi fenolik ve flavonoid içeriğine sahipken, memeli dokusu ve hematolojik parametreler üzerinde belirgin toksik yan etkilere sahip olmamıştır.

🔖

Cite This Article

MURTAZA, I., REHAN, T., TAHIRA, R., ULLAH, H., TAREEN, U., REHAN, T., ANEES, M., SULTAN, A. (2022). In vitro bioactivities and subacute toxicity study of O. basilicum, T. vulgaris and R. officinalis. Türk Biyokimya Dergisi, Cilt: 43 - Sayı: 4, 447–455. https://ajindex.com/articles/620962

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Tawaha K, Alali FQ, Gharaibeh M, Mohammad M, El-Elimat T. Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chem 2007;104:1372–8
  2. Peng Y, Yuan J, Liu F, Ye J. Determination of active components in rosemary by capillary electrophoresis with electrochemical detection. J Pharm Biomed Anal 2005;39:431–7
  3. Moreira M, Ponce A, Del Valle C, Roura S. Inhibitory parameters of essential oils to reduce a foodborne pathogen. LWT-Food Sci Technol 2005;38:565–70
  4. Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev 2009;2:270–8
  5. Sharma S, Bhadange D. Antimicrobial potential of Lamiaceae members. Int J Pharma Sci 2013;3:324–7
  6. Orhan I, Aslan S, Kartal M, Şener B, Başer KH. Inhibitory effect of Turkish Rosmarinus officinalis L. on acetylcholinesterase and butyrylcholinesterase enzymes. Food Chem 2008;108:663–8
  7. Soyal D, Jindal A, Singh I, Goyal P. Modulation of radiationinduced biochemical alterations in mice by rosemary (Rosemarinus officinalis) extract. Phytomedicine 2007;14:701–5
  8. Marwat SK, Khan MS, Ghulam S, Anwar N, Mustafa G, Usman K. Phytochemical constituents and pharmacological activities of sweet Basil-Ocimum basilicum L. (Lamiaceae). Asian J Chem 2011;23:3773–82
  9. Shirazi MT, Gholami H, Kavoosi G, Rowshan V, Tafsiry A. Chemical composition, antioxidant, antimicrobial and cytotoxic activities of Tagetes minuta and Ocimum basilicum essential oils. Food Sci Nutr 2014;2:146–55
  10. Lee S-J, Umano K, Shibamoto T, Lee K-G. Identification of volatile components in basil (Ocimum basilicum L.) and thyme leaves (Thymus vulgaris L.) and their antioxidant properties. Food Chem 2005;91:131–7
  11. Baranauskienė R, Venskutonis PR, Viškelis P, Dambrauskienė E. Influence of nitrogen fertilizers on the yield and composition of thyme (Thymus vulgaris). J Agric Food Chem 2003;51:7751–8
  12. Behnia M, Haghighi A, Komeylizadeh H, Tabaei S-J, Abadi A. Inhibitory effects of Iranian Thymus vulgaris extracts on in vitro growth of Entamoeba histolytica. Korean J Parasitol 2008;46:153–6
  13. Mahmoud AA, Al-Shihry SS, Son BW. Diterpenoid quinones from Rosemary (Rosmarinus officinalis L.). Phytochemistry 2005;66:1685–90
  14. Atanassova M, Georgieva S, Ivancheva K. Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs. J Univ Chem Technol Metallurgy 2011;46:81–8
  15. Kulisic T, Radonic A, Katalinic V, Milos M.