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Journals / Türk Biyokimya Dergisi / 2020 / Cilt: 45 - Sayı: 3

Serum NOX-2 concentrations andparaoxanase-1 activity in subclinicalhypothyroidism: a pilot study

Subklinik hipotiroidizmli hastalarda serum NOX-2 konsantrasyonlari ve paraoksonaz-1 aktivitesi: bir pilot çalişma

Halef Okan DOĞAN
Gülhan DUMAN
Pages
271–276
Publication Date
2022-11-06
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Larger prospective studies required to confirm these findings.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Subclinical hypothyroidism (SH) is one of the most prevalent endocrine disorders.
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Key Findings & Data:Although recent data suggest an imbalanced oxidative status in SH, the mechanisms of increased oxidative stress are poorly figured out.
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Conclusion & Impact:Larger prospective studies required to confirm these findings.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Subclinical hypothyroidism (SH) is one of the most prevalent endocrine disorders. Although recent data suggest an imbalanced oxidative status in SH, the mechanisms of increased oxidative stress are poorly figured out. The primary goal of this research was to analyze potential sources of ROS and the relationship between serum NOX-2 levels and paraoxonase-1 (PON-1) activity in SH. Serum lipid changes in SH patients which had been determined were compared to healthy control group.Materials and methods: Thirty-one patients diagnosed with SH and 30 healthy controls were included in the study. The quantitative sandwich ELISA was used for the detection of serum NOX-2 levels. Spectrophotometric method was used to determine serum PON-1 activity.Results: Higher median serum NOX-2 levels were determined in patients than in the control group (p = 0.004). Lower median serum PON-1 activity was determined in patients as to aforementioned control group (p < 0.0001). As a consequence, no statistically remarkable correlation was identified between PON-1 activity and NOX-2 levels. Triglyceride (TG) concentrations were determined as superior in patients to control group (p < 0.0001).Conclusion: Over-production of NOX-2 and decreased PON-1 activity contribute to the increased oxidative stress in SH patients. Larger prospective studies required to confirm these findings.

Özet

Amaç: Subklinik hipotiroidi (SH) en yaygın endokrin has talıklardan biridir. Her ne kadar yeni veriler SH’de den gesiz bir oksidatif durum gösterse de, oksidatif stresinarttığı mekanizmalar henüz iyi çözümlenmiş değildir.Bu araştırmanın temel amacı, reaktif oksidasyon ürün lerinin (ROS) potansiyel kaynaklarını ve SH’deki serumNOX-2 düzeyleri ve paraoksonaz-1 (PON-1) aktivitesiarasındaki ilişkiyi analiz etmektir. Yanı sıra SH hastala rında serum lipit değişiklikleri sağlıklı kontrol grubu ilekarşılaştırmaktır. Gereç ve Yöntem: SH tanısı almış 31 hasta ve 30 sağlıklıkontrol çalışmaya dahil edildi. Kantitatif sandviç ELISA,serum NOX-2 seviyelerinin tespiti için kullanıldı. SerumPON-1 aktivitesini belirlemek için spektrofotometrikyöntem kullanıldı. Bulgular: Hastalarda ortanca serum NOX-2 düzeylerikontrol grubuna göre daha yüksek bulundu (p = 0,004).Kontrol grubuna göre hastalarda düşük median serumPON-1 aktivitesi belirlendi (p < 0,0001). Sonuç olarak,PON-1 aktivitesi ile NOX-2 seviyeleri arasında istatistikselolarak kayda değer bir korelasyon saptanmadı. Trigliserit(TG) konsantrasyonları kontrol grubundaki hastalardanyüksek bulundu (p < 0,0001). Sonuç: NOX-2’nin aşırı üretimi ve azalmış PON-1 aktivitesi,SH hastalarında oksidatif stresin artmasına katkıda bulu nur. Bu bulguları doğrulamak için daha geniş prospektifçalışmalar gereklidir.

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Cite This Article

DOĞAN, H. O., DUMAN, G. (2022). Serum NOX-2 concentrations andparaoxanase-1 activity in subclinicalhypothyroidism: a pilot study. Türk Biyokimya Dergisi, Cilt: 45 - Sayı: 3, 271–276. https://ajindex.com/articles/620806

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