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Journals / Türk Biyokimya Dergisi / 2021 / Cilt: 46 - Sayı: 1

Reticulocyte hemoglobin equivalent in differential diagnosis of iron deficiency, iron deficiency anemia and β thalassemia trait in children

Retiküulosit hemoglobin eşdeğerinin çocuklarda demir eksikliği, demir eksikliği anemisi ve β talasemi taşıyıcılığı ayırıcı tanısında yeri

Yeter DÜZENLİ KAR
Konca ALTINKAYNAK
Pages
45–51
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In particular, patients with low Ret-He who don’t respond to iron therapy should be examined for β-TT.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: This study investigated the diagnostic power of reticulocyte hemoglobin equivalent (Ret-He) in the dif ferential diagnosis of hypochromic microcytic anemia to differentiate iron deficiency anemia (IDA) and thalassemia trait (TT) based on the traditionally used erythrocyte index and formulas.
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Key Findings & Data:Methods: Twenty-six children with iron deficiency (ID), 26 with IDA, 33 with β-TT, 41 healthy children were assessed.
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Conclusion & Impact:In particular, patients with low Ret-He who don’t respond to iron therapy should be examined for β-TT.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: This study investigated the diagnostic power of reticulocyte hemoglobin equivalent (Ret-He) in the dif ferential diagnosis of hypochromic microcytic anemia to differentiate iron deficiency anemia (IDA) and thalassemia trait (TT) based on the traditionally used erythrocyte index and formulas. Methods: Twenty-six children with iron deficiency (ID), 26 with IDA, 33 with β-TT, 41 healthy children were assessed. Complete blood count parameters, Ret-He, immature reticulocyte fraction (IRF), low-fluorescence ratio (LFR), Mentzer’s indexes (MI) were evaluated. The diagnostic power of Ret-He in distinguishing between IDA and β-TT was investigated using ROC analysis. Results: Ret-He levels were (median(Q1-Q3)) 20.6(19.7–21.5) pg in β-TT, 16.1(13.1–20) pg in IDA, 29.7(27.2–30.7) pg in ID, 30.5(29.8–31.7) pg in healthy controls. Based on ROC analysis, diagnostic power for distinguishing between IDA and β-TT was determined as RBC>MI>Ret-He>RDW>LFR>IRF. The highest sensitivity and specificity for differential diagnosis was obtained when the Ret-He cut-off value was 18.2pg. The AUC (95%CI) value was calculated as 0.765(0.637–0.866), and a statistically significant difference was found between groups (p10.42 can be safely used to distinguish IDA from β-TT. In particular, patients with low Ret-He who don’t respond to iron therapy should be examined for β-TT.

Özet

Amaç: Bu çalışmada, hipokrom mikrositer anemi ayırıcı tanısında, demir eksikliği anemisi (IDA) ve Talasemi taşı yıcılığı (TT) durumlarının ayırt edilmesinde retikülosit he moglobin eşdeğerinin (RET-He), klasik olarak kullanılan eritrosit indeks ve formüllerine göre diagnostik gücü araştırılmıştır. Gereç-Yöntem: Çalışmaya 26 demir eksikliği (ID), 26 IDA, 33 β-TT, 41 sağlıklı çocuk alındı. Hastaların tam kan sayımı parametreleri, Ret-He, immatur retikülosit fraksiyonu (IRF), low fluorescence ratio (LFR), Mentzer Indeksi (MI) değerlendirildi. Receiver operating characteristic (ROC) analizi ile Ret-He’nin IDA ve β-TT’nı ayırt etmede diagno sitik gücü araştırıldı. Bulgular: Çalışmamızda Ret-He düzeyi β-TT; median (Q1-Q3) 20.6 (19.7–21.5) pg, IDA; 16.1 (13.1–20) pg, ID; 29.7 (27.2–30.7) pg, sağlıklı kontroller; 30.5 (29.8–31.7) pg olarak tespit edildi. ROC analizine göre IDA ve β-TT’nı ayırt etmede diagnostik gücü en yüksekten en düşüğe doğru RBC>MI>Ret He>RDW>LFR>IRF olarak tespit edildi. Ret-He cut-off değeri 18.2 pg alındığında IDA-β-TT ayırıcı tanısında AUC (95%CI) değeri 0.765(0.637–0.866) olarak en yüksek sensitive ve spesitiviteye sahipti ve her 2 grup arasında istatistiksel anlamlı fark olduğu bulundu (p 10.42 olması IDA’yı β-TT’nden ayırt etmede güvenle kullanılabilir. Özellikle Ret-He düşük saptanan ve demir tedavisine yanıt vermeyen hastalar β-TT açısından tetkik edilmelidir.

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Cite This Article

KAR, Y. D., ALTINKAYNAK, K. (2022). Reticulocyte hemoglobin equivalent in differential diagnosis of iron deficiency, iron deficiency anemia and β thalassemia trait in children. Türk Biyokimya Dergisi, Cilt: 46 - Sayı: 1, 45–51. https://ajindex.com/articles/620686

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