AI Summary & Key Takeaways
AI InsightMethod II was used for the quantification of MES,based on measurement of the absorbance of yellow chromogen at 400 nm, which is formed by the condensation reaction of the primary aminogroup of MES with salicylaldehyde reagent (SA) (Schiff base formation).Results: Both methods obeyed Beer’s law in the concentration range of 0.5-4.5 μg/mL and 0.2-1.7 μg/mL with good correlation coefficients of 0.9974and 0.998 for methods I and II, respectively.Conclusion: The developed method is simple, sensitive, and specific, which was validated statistically as per ICH guidelines, and can be used in theroutine analysis of LOR and MES in pharmaceutical dosage forms.
📌 3 Key Takeaways
Abstract
Objectives: Simple, specific, accurate, precise, sensitive, and cost effective spectrophotometric methods were developed and validated forquantification of the drugs lornoxicam (LOR) and mesalamine (MES) in pure form and in pharmaceutical formulations.Materials and Methods: A Shimadzu UV-1800 double-beam UV-Visible spectrophotometer having a spectral bandwidth of 0.1 nm with wavelengthaccuracy ±0.1 nm with a pair of 1 cm path length matched quartz cells was used to measure the absorbance of the resulting solution.Method I was used for the quantification of LOR, based on measurement of the absorbance of bluish green chromogen complex at 760 nm, which isformed by the reaction of LOR with ferric chloride and potassium ferricyanide (redox technique). Method II was used for the quantification of MES,based on measurement of the absorbance of yellow chromogen at 400 nm, which is formed by the condensation reaction of the primary aminogroup of MES with salicylaldehyde reagent (SA) (Schiff base formation).Results: Both methods obeyed Beer’s law in the concentration range of 0.5-4.5 μg/mL and 0.2-1.7 μg/mL with good correlation coefficients of 0.9974and 0.998 for methods I and II, respectively.Conclusion: The developed method is simple, sensitive, and specific, which was validated statistically as per ICH guidelines, and can be used in theroutine analysis of LOR and MES in pharmaceutical dosage forms.
Özet
Amaç: Lornoksikam (LOR) ve mesalaminin (MES) hem saf formda hemde farmasötik formülasyonlarda tayini için basit, spesifik, doğru, duyarlı ve düşük maliyetli spektrofotometrik yöntemler, geliştirmek ve valide etmektir. Gereç ve Yöntemler: Elde edilen çözeltilerin absorbansları Shimadzu UV-1800 çift ışınlı spektrofotometrede 0,1 nm spektral bant genişliği ve ±0,1 nm hassasiyetle 1 cm’lik kuvars küvetler kullanılarak ölçüldü. Bulgular: Metot I, LOR’nin demir III klorür ve potasyum ferrisiyanür (redoks tekniği) ile reaksiyonu sonucu oluşan mavimsi yeşil renkli kromojen kompleksinin absorbansının 760 nm’de ölçümüne dayanan yöntemdir. Metot II, MES’nin primer amino grubunun salisilaldehit reaktifi (SA) (Schiff baz oluşumu) ile kondensasyon reaksiyonundan oluşan sarı renkli kromojenin absorbansının 400 nm’de ölçümüne dayanan yöntemdir. Metot I ve II için; her iki yöntemde de, sırasıyla 0,5-4,5 μg/mL ve 0,2-1,7 μg/mL’lik konsantrasyon aralıklarında, Beer yasasına sırasıyla 0,9974 ve 0,9980‘lık korelasyon katsayıları ile iyi bir uygunluk göstermişlerdir. Sonuç: Geliştirilen yöntemler; LOR ve MES’nin farmasötik dozaj formlarının rutin analizinde kullanılabilecek ve ICH yönergelerine göre valide edilmiş basit, hassas ve spesifik yöntemlerdir.