Journals / Turkish Journal of Medical Sciences / 2007 / Cilt: 37 - Sayı: 1

Development of a highly sensitive ELISAfor quantification of hepatitis B virus (HBV) surface antigen ( HBsAg)

Hepatit B virus (HBV) yüzey antijejeninin (HBsAg) ölçümü amacı ile yüksek düzeyde hassas bir ELISA kitinin geliştirilmesi

Pages
87–92
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusions: These reliable results of the newly established HBsAg ELISA system make it a promising candidate for diagnostic as well as research purposes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background: Hepatitis B Virus (HBV) infection is of global importance and many studies are aimed to develop more sensitive diagnostic tools and improve therapeutic options.
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Key Findings & Data:Materials and Methods: A hybridoma (clone 4D1) secreting anti-HBs IgG2a monoclonal antibody (mAb) was used for the establishment of our in-house ELISA.
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Conclusion & Impact:Conclusions: These reliable results of the newly established HBsAg ELISA system make it a promising candidate for diagnostic as well as research purposes.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: Hepatitis B Virus (HBV) infection is of global importance and many studies are aimed to develop more sensitive diagnostic tools and improve therapeutic options. HBV infection is diagnosed and followed-up basically by serological studies, among which the enzyme-linked immunosorbent assay (ELISA) is widely used. Aim: To develop a sensitive HBsAg ELISA for both research and diagnostic purposes. Materials and Methods: A hybridoma (clone 4D1) secreting anti-HBs IgG2a monoclonal antibody (mAb) was used for the establishment of our in-house ELISA. Tests were performed for both cell culture medium and human sera. The hepatoma cell lines of human origin PLC/PRF/5 and Hep G2 were used for validation assays. Tests for human sera were run concurrently with the Access® Immunoassay System. Results: The in-house ELISA system had an analytical sensitivity less than 0.41 ng/ml for both human sera and cell culture medium. The performance comparison of our in-house ELISA system with the Access® Immunoassay System even for samples with s/co levels of 1.5-13.7 gave a significant correlation. Conclusions: These reliable results of the newly established HBsAg ELISA system make it a promising candidate for diagnostic as well as research purposes.

Özet

Background: Hepatitis B Virus (HBV) infection is of global importance and many studies are aimed to develop more sensitive diagnostic tools and improve therapeutic options. HBV infection is diagnosed and followed-up basically by serological studies, among which the enzyme-linked immunosorbent assay (ELISA) is widely used. Aim: To develop a sensitive HBsAg ELISA for both research and diagnostic purposes. Materials and Methods: A hybridoma (clone 4D1) secreting anti-HBs IgG2a monoclonal antibody (mAb) was used for the establishment of our in-house ELISA. Tests were performed for both cell culture medium and human sera. The hepatoma cell lines of human origin PLC/PRF/5 and Hep G2 were used for validation assays. Tests for human sera were run concurrently with the Access® Immunoassay System. Results: The in-house ELISA system had an analytical sensitivity less than 0.41 ng/ml for both human sera and cell culture medium. The performance comparison of our in-house ELISA system with the Access® Immunoassay System even for samples with s/co levels of 1.5-13.7 gave a significant correlation. Conclusions: These reliable results of the newly established HBsAg ELISA system make it a promising candidate for diagnostic as well as research purposes.

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Cite This Article

ARAL, L. A., KARAKUŞ, R., AYBAY, C., BAŞTÜRK, B. (2022). Development of a highly sensitive ELISAfor quantification of hepatitis B virus (HBV) surface antigen ( HBsAg). Turkish Journal of Medical Sciences, Cilt: 37 - Sayı: 1, 87–92. https://ajindex.com/articles/611844

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