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Journals / Turkish Journal of Medical Sciences / 2013 / Cilt: 43 - Sayı: 4

Cytokine associated neopterin response of peripheral blood mononuclear cells to in vitro Epstein–Barr virus transformation process of B lymphocytes

Latife Arzu ARAL
Ayşegül Atak YÜCEL
Pages
562–568
Publication Date
2013-04-01
DOI
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Conclusion: According to these results, neopterin levels, which increased with time and independently of EBV transformation, may be a helpful marker for evaluating the acute response to viral infection, but not for B lymphocyte transformation

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The Epstein-Barr Virus (EBV) is a human herpes virus that infects B lymphocytes latently and immortalises the cells due to transformation.
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Key Findings & Data:Materials and methods: Mononuclear cells were isolated from peripheral blood samples of healthy donors and transformed with an EBV secreting cell-line, B95-8.
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Conclusion & Impact:Conclusion: According to these results, neopterin levels, which increased with time and independently of EBV transformation, may be a helpful marker for evaluating the acute response to viral infection, but not for B lymphocyte transformation
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: B lymphocyte transformation · EBV · in vitro

Abstract

The Epstein-Barr Virus (EBV) is a human herpes virus that infects B lymphocytes latently and immortalises the cells due to transformation. EBV infection is asymptomatic in childhood, while it may cause a self-limiting lymphoproliferative disorder in adolescence. This study aimed to show the association between in vitro transformation of B lymphocytes via EBV and the levels of neopterin with different accompanying cytokine responses. Materials and methods: Mononuclear cells were isolated from peripheral blood samples of healthy donors and transformed with an EBV secreting cell-line, B95-8. Neopterin, interferon (IFN)-g, tumour necrosis factor (TNF)-a, and interleukin (IL)-6 levels were measured in culture supernatants using enzyme immunoassay. Results: Neopterin levels increased dependent on time and independent of EBV transformation. In transformed cell cultures, TNF-a and IFN-g levels reached their peak in the first week and decreased in the third week; however there were no significant differences between the 3 weeks. IL-6 reached its maximum level in the third week. Conclusion: According to these results, neopterin levels, which increased with time and independently of EBV transformation, may be a helpful marker for evaluating the acute response to viral infection, but not for B lymphocyte transformation

Özet

The Epstein-Barr Virus (EBV) is a human herpes virus that infects B lymphocytes latently and immortalises the cells due to transformation. EBV infection is asymptomatic in childhood, while it may cause a self-limiting lymphoproliferative disorder in adolescence. This study aimed to show the association between in vitro transformation of B lymphocytes via EBV and the levels of neopterin with different accompanying cytokine responses. Materials and methods: Mononuclear cells were isolated from peripheral blood samples of healthy donors and transformed with an EBV secreting cell-line, B95-8. Neopterin, interferon (IFN)-g, tumour necrosis factor (TNF)-a, and interleukin (IL)-6 levels were measured in culture supernatants using enzyme immunoassay. Results: Neopterin levels increased dependent on time and independent of EBV transformation. In transformed cell cultures, TNF-a and IFN-g levels reached their peak in the first week and decreased in the third week; however there were no significant differences between the 3 weeks. IL-6 reached its maximum level in the third week. Conclusion: According to these results, neopterin levels, which increased with time and independently of EBV transformation, may be a helpful marker for evaluating the acute response to viral infection, but not for B lymphocyte transformation

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Cite This Article

ARAL, L. A., YÜCEL, A. A. (2013). Cytokine associated neopterin response of peripheral blood mononuclear cells to in vitro Epstein–Barr virus transformation process of B lymphocytes. Turkish Journal of Medical Sciences, Cilt: 43 - Sayı: 4, 562–568. https://ajindex.com/articles/400902

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