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Journals / Turkish Journal of Immunology / 2018 / Cilt: 6 - Sayı: 1

Identification of Hemagglutinin Protein from Streptococcus pneumoniae Pili as a Vaccine Candidate by Proteomic Analysis

Proteomic Analiz ile Streptococcus pneumoniae’dan Aşı Adayı olarak Hemagglutinin Proteininin Tanımlanması

Kusworini HANDONO
Sanarto SANTOSO
Sumarno Reto PRAWİRO
Diana Chusna MUFİDA
Pages
8–15
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Computational bioinformatics analysis by Mascot Server revealed thishemagglutinin matched with the Rrgb protein of S. pneumoniae (gi: 299856655).Conclusion: The analysis of its antigenicity, and epitope mapping showed that a novel protein was identified as thepilus backbone (Rrgb) of Streptococcus pneumoniae, and has a high antigenicity suitable for further development as avaccine candidate.

📌 3 Key Takeaways

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Objective & Hypothesis:Introduction: Streptococcus pneumoniae is a Gram-positive, bacterial human pathogen that causes a large numberof respiratory tract infections, such as pneumonia and sinusitis, as well as invasive diseases such as septicemia andmeningitis.
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Key Findings & Data:After a hemagglutinination assay with mouse erythrocytes,the hemagglutinin protein was identified with Liquid Chromatography/Mass Spectrometry, along with assessments ofantigenicity and epitope mapping.Results: SDS-PAGE showed that pili protein had major bands of molecular weights of 67, 54, 25, and 11 kDa.
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Conclusion & Impact:Computational bioinformatics analysis by Mascot Server revealed thishemagglutinin matched with the Rrgb protein of S. pneumoniae (gi: 299856655).Conclusion: The analysis of its antigenicity, and epitope mapping showed that a novel protein was identified as thepilus backbone (Rrgb) of Streptococcus pneumoniae, and has a high antigenicity suitable for further development as avaccine candidate.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Introduction: Streptococcus pneumoniae is a Gram-positive, bacterial human pathogen that causes a large numberof respiratory tract infections, such as pneumonia and sinusitis, as well as invasive diseases such as septicemia andmeningitis. Among the surface-exposed virulence factors, the pneumococcal pilus has been shown to play a role inhost-cell adhesion. Hemagglutinin activity has been shown to correlate with adhesion ability. The purpose of this studywas to identify of S. pneumoniae pili proteins with hemagglutinin activity, and to proteonomically analyze such proteinsas potential vaccine candidates.Materials and Methods: The purification of pili was carried out by a pilus cutter method. After SDS-PAGE separation,pili proteins were purified by electroelution and dialysis. After a hemagglutinination assay with mouse erythrocytes,the hemagglutinin protein was identified with Liquid Chromatography/Mass Spectrometry, along with assessments ofantigenicity and epitope mapping.Results: SDS-PAGE showed that pili protein had major bands of molecular weights of 67, 54, 25, and 11 kDa. The54 kDa pili protein was the hemagglutinin. Computational bioinformatics analysis by Mascot Server revealed thishemagglutinin matched with the Rrgb protein of S. pneumoniae (gi: 299856655).Conclusion: The analysis of its antigenicity, and epitope mapping showed that a novel protein was identified as thepilus backbone (Rrgb) of Streptococcus pneumoniae, and has a high antigenicity suitable for further development as avaccine candidate.

Özet

Giriş: Streptococcus pneumoniae, pnömoni ve sinüzit gibi solunum yolu infeksiyonu olduğu kadar septisemi ve menenjit gibi daha ciddi infeksiyonlara da yol açan Gram-pozitif bir bakteridir. Virulansı belirleyen yüzey ile ilişkili öğeleri içinde, pnömokok pilusu önemli bir rol oynar. Hemaglütünin aktivitesinin ise, bakterinin yapışma özelliği ile ilişkili olduğu gösterilmiştir ve bu proteinlerin proteom ile irdelenmesi, oluşturulabilecek bir aşıda kullanılabilmelerini sağlayabilir. Gereçler ve Yöntemler: Pilusların saflaştırılması, pilusların kesilmesi ile yapılmıştır. Pili proteini SDS-PAGE kullanarak, elektroelusyon ve diyaliz ile saflaştırılmıştır. Fare eritrositleri ile yapılan hemaglütinasyon testinden sonra hemaglütinin proteini, epitop haritalaması ve antijenik özelliği Sıvı Kromatografisi ve Kitle Spektrometresi ile tanımlandı. Bulgular: SDS-PAGE yöntemi ile pili proteinleri ana bantları 67, 52, 25 ve 11kDA moleküler ağırlığında saptandı. 54 KDa ağırlığındaki pilus proteini hemaglütinin antijeni idi. Mascot sunucusu ile yapılan hesaplamalı biyobilgi analizi bu hemaglutininin S. pneumoniae’inin Rrgb proteini ile eşleştiğini gösterdi. Sonuç: Antijen özelliği ve epitop haritalama Streptococcus pneumoniae’dan pilus omurgası (Rrgb) olarak tanımlanan bu yeni proteinin yüksek antijenik özelliğinden dolayı aşı geliştirmede uygun bir aday olabileceğini göstermektedir.

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Cite This Article

HANDONO, K., SANTOSO, S., PRAWİRO, S. R., MUFİDA, D. C. (2022). Identification of Hemagglutinin Protein from Streptococcus pneumoniae Pili as a Vaccine Candidate by Proteomic Analysis. Turkish Journal of Immunology, Cilt: 6 - Sayı: 1, 8–15. https://ajindex.com/articles/607214

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