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Journals / Turkish Journal of Immunology / 2020 / Cilt: 8 - Sayı: 2

Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions

Eritrosit Süspansiyonlarında Depolanma Sürecinin CD4+/CD8+ T Lenfositleri Oranı Üzerine Etkisi

Fatma Ezgi CAN
Yasemin HEPER
Salih Haldun BAL
Levent Tufan KUMAŞ
Ferah BUDAK
Güher GÖRAL
Haluk Barbaros ORAL
Pages
44–49
Publication Date
2022-11-06
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Decrease of CD3 (p=0,001), CD4 (p

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Introduction: Some immunologic changes in the recipient derived by allogeneic blood transfusion (ABT) are called Transfusion Related Immune Modulation (TRIM).
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Key Findings & Data:Despite the exact mechanisms of these changes are not known, it is thought that ABT causes a decrease of CD4/CD8 ratio in the recipient.
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Conclusion & Impact:Decrease of CD3 (p=0,001), CD4 (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Introduction: Some immunologic changes in the recipient derived by allogeneic blood transfusion (ABT) are called Transfusion Related Immune Modulation (TRIM). Despite the exact mechanisms of these changes are not known, it is thought that ABT causes a decrease of CD4/CD8 ratio in the recipient. This study aimed to determine the CD4/CD8 ratio in stored erythrocyte suspensions (ES) and to obtain new information about TRIM mechanisms. Materials and Methods: Whole blood components used in our study were collected from 10 healthy volunteers. ES’ obtained from whole blood were divided into three equal aliquots. Test samples which were related to 0th, 21st and 42nd storage days were prepared from these aliquots. CD3, CD4 and CD8 surface markers in peripheral blood mononuclear cells were investigated with flow-cytometer in these test samples. Results: Our data were evaluated according to storage days. Decrease of CD3 (p=0,001), CD4 (p

Özet

Giriş: Allojeneik kan transfüzyonunun (AKT) alıcıda neden olduğu bazı immünolojik değişikliklere transfüzyonla ilişkili immunomodülasyon (TRIM) adı verilmektedir. Bu değişikliklerin mekanizması tam olarak bilinmese de, AKT’nin alıcının mononülear kan hücrelerindeki CD4/CD8 ifadesi oranında azalmaya yol açtığına inanılmaktadır. Bu çalışmada depolanan eritrosit süspansiyonlarından elde edilen mononükleer hücrelerdeki (ES) CD4/CD8 ifadesi oranını belirlemek ve TRIM mekanizmalarına yönelik yeni bilgiler edinmek amaçlanmıştır. Gereç ve Yöntemler: Çalışmamızda kullanılan tam kanlar sağlıklı 10 gönüllüden elde edilmiştir. Bu tam kanlardan elde edilen ES’ler eşit üç parçaya bölünmüş ve ES’lerin 0, 21 ve 42. raf günlerine yönelik test örnekleri bu parçalardan elde edilmiştir. Test örneklerdeki CD3, CD4 ve CD8 yüzey belirteçleri akan hücre ölçer yardımıyla incelenmiştir. Bulgular: Verilerimiz ES’lerin depolanma günlerine gore değerlendirilmiştir. Sıfırıncı gün örneklerine göre 21. gün örneklerinde CD3 (p=0,001), CD4 (p

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Cite This Article

CAN, F. E., HEPER, Y., BAL, S. H., KUMAŞ, L. T., BUDAK, F., GÖRAL, G., ORAL, H. B. (2022). Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions. Turkish Journal of Immunology, Cilt: 8 - Sayı: 2, 44–49. https://ajindex.com/articles/607173

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