High KEAP1, NRF2 and Low HO-1 Serum Levels in Children with Autism

HO-1, KEAP1, and NRF2 levels were determined in the biochemistry laboratory using the ELISA technique.Results: HO-1 levels were significantly lower in patients aged 3–12 years compared to controls aged 3–12, while KEAP1 and NRF2 levels were significantly higher (p=0.020, p0.05).Conclusion: This study suggests that oxidative stress is higher in children with ASD and that HO-1 levels are insufficient to achieve oxidative balance.

📌 3 Key Takeaways

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Objective & Hypothesis:Introduction: The purpose of our study was to investigate heme oxygenase-1 (HO-1), nuclear factor erythroid-2-related factor 2 (NRF2), and kelch-like ECH-associated protein 1 (KEAP1) levels in children with autism spectrum disorder (ASD) and to reveal their association with the severity of autism.Methods: This study measured serum HO-1, KEAP1, and NRF2 levels in 43 patients with ASD (aged 3–12 years) and in 41 age– and gendermatched healthy controls.

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Key Findings & Data:ASD severity was rated using the Childhood Autism Rating Scale (CARS).

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Conclusion & Impact:HO-1, KEAP1, and NRF2 levels were determined in the biochemistry laboratory using the ELISA technique.Results: HO-1 levels were significantly lower in patients aged 3–12 years compared to controls aged 3–12, while KEAP1 and NRF2 levels were significantly higher (p=0.020, p0.05).Conclusion: This study suggests that oxidative stress is higher in children with ASD and that HO-1 levels are insufficient to achieve oxidative balance.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

Introduction: The purpose of our study was to investigate heme oxygenase-1 (HO-1), nuclear factor erythroid-2-related factor 2 (NRF2), and kelch-like ECH-associated protein 1 (KEAP1) levels in children with autism spectrum disorder (ASD) and to reveal their association with the severity of autism.Methods: This study measured serum HO-1, KEAP1, and NRF2 levels in 43 patients with ASD (aged 3–12 years) and in 41 age– and gendermatched healthy controls. ASD severity was rated using the Childhood Autism Rating Scale (CARS). HO-1, KEAP1, and NRF2 levels were determined in the biochemistry laboratory using the ELISA technique.Results: HO-1 levels were significantly lower in patients aged 3–12 years compared to controls aged 3–12, while KEAP1 and NRF2 levels were significantly higher (p=0.020, p0.05).Conclusion: This study suggests that oxidative stress is higher in children with ASD and that HO-1 levels are insufficient to achieve oxidative balance.

Özet

Amaç: Çalışmamızın amacı otizm spektrum bozukluğu (OSB) tanılıçocuklarda heme oksijenaz-1 (HO-1), nükleer faktör eritroid-2 ileilişkili faktör 2 (NRF2) ve kelch benzeri ECH ile ilişkili protein 1 (KEAP1)düzeylerini araştırmak otizmin ciddiyeti ile ilişkilerini ortaya koymaktır. Yöntem: Bu çalışmada, OSB tanılı 43 hasta (yaş 3-12 yaş) ve yaş, cinsiyetuyumlu 41 sağlıklı kontrolde serum HO-1, KEAP1 ve NRF2 düzeyleriölçülmüştür. OSB şiddeti, çocukluk otizm değerlendirme ölçeği (ÇODÖ)kullanılarak derecelendirildi. ELISA tekniği kullanılarak biyokimyalaboratuarında HO-1, KEAP1 ve NRF2 seviyeleri belirlendi. Bulgular: 3-12 yaş arası hastalarda HO-1 düzeyleri 3-12 yaş arasıkontrollere göre anlamlı derecede düşükken, KEAP1 ve NRF2 düzeylerianlamlı olarak yüksek bulundu (sırasıyla p = 0.020, p 0.05). Sonuç: Bu çalışma, OSB’li çocuklarda oksidatif stresin daha yüksekolduğunu ve HO-1 seviyelerinin oksidatif dengeyi sağlamak için yetersizolduğunu göstermektedir.

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Cite This Article

ÇELİK, H., Koyuncu, İ., KİRMİT, A., AKALTUN, İ., AYAYDIN, H., TAKATAK, H. (2022). High KEAP1, NRF2 and Low HO-1 Serum Levels in Children with Autism. Nöropsikiyatri Arşivi, Cilt: 57 - Sayı: 4, 274–279. https://ajindex.com/articles/583270

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