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Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2020 / Cilt: 26 - Sayı: 2

Effects of RNase III rncS Gene Deletion on Stress Response, Biofilm Formation and Virulence of Listeria monocytogenes

Listeria monocytogenes’in RNase III rncS Geninin Silinmesinin Stres Tepkisi, Biyofilm Oluşumu ve Virülansı Üzerine Etkileri

Jun QIAO
Qingling MENG
Mengfan QIAO
Xingxing ZHANG
Xuepeng CAI
Lixia WANG
Yehui WU
Jing GUO
Xifeng WANG
Jie LI
Pages
269–277
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

When compared with LM EGD-e, the adaptability of LM-ΔrncS was significantly reduced (P

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The ribonuclease III (RNase III) is an important enzyme system that regulates non-coding RNA (ncRNA) levels.
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Key Findings & Data:In this study, LM-ΔrncS gene deletion strain was investigated by gene overlap extension PCR (SOE-PCR) and homologous recombination techniques.
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Conclusion & Impact:When compared with LM EGD-e, the adaptability of LM-ΔrncS was significantly reduced (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The ribonuclease III (RNase III) is an important enzyme system that regulates non-coding RNA (ncRNA) levels. In this study, LM-ΔrncS gene deletion strain was investigated by gene overlap extension PCR (SOE-PCR) and homologous recombination techniques. The environmental stress response, biofilm formation and virulence were determined and compared between the deletion strain LM-ΔrncS and the parental strain LM EGD-e. When compared with LM EGD-e, the adaptability of LM-ΔrncS was significantly reduced (P

Özet

Ribonükleaz III (RNase III), kodlama yapmayan RNA (ncRNA) seviyelerini düzenleyen önemli bir enzim sistemidir. Bu çalışmada, LM-ΔrncS gen silme suşu, gen örtüşme uzatma PCR (SOE-PCR) ve homolog rekombinasyon teknikleri ile incelendi. Çevresel stres tepkisi, biyofilm oluşumu ve virülans düzeyi belirlenerek silme suşu LM-ΔrncS ile ana suş LM EGD-e karşılaştırıldı. LM EGD-e ile karşılaştırıldığında, LM-ΔrncS’nin adapte olabilirliği 30°C/42°C, pH 9, %5 NaCl, %3.8 etanol ve %0.1 H202 stresi altında önemli ölçüde azaldı (P

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Cite This Article

QIAO, J., MENG, Q., QIAO, M., ZHANG, X., CAI, X., WANG, L., WU, Y., GUO, J., WANG, X., LI, J. (2022). Effects of RNase III rncS Gene Deletion on Stress Response, Biofilm Formation and Virulence of Listeria monocytogenes. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 26 - Sayı: 2, 269–277. https://ajindex.com/articles/569601

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References (1)

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