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Journals / Journal of Clinical and Analytical Medicine / 2012 / Cilt: 3 - Sayı: 4

Diabetik sıçan retina ve siliyer cisiminde aquaporin-1 lokalizasyonu

Localisation of aquaporin-1 In diabetic rat retina and ciliary body

Erkan SÖĞÜT
Huseyin ORTAK
Sevil ÇAYLİ
Ufuk TAŞ
Fatih EKİCİ
Duygu ÇAKIL
Pages
438–441
Publication Date
2022-10-18
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

Discussion: Our results indicate that Aquaporin 1 immunoreactivity in the diabetic rat retina and ciliary body significantly increases compared to non-diabetic rats.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Aquaporins are membrane water channels that play critical roles in controlling the water contents of cells.
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Key Findings & Data:We aimed to investigate the distribution of aquaporin 1 in non diabetic and diabetic rat retina and ciliary body.
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Conclusion & Impact:Discussion: Our results indicate that Aquaporin 1 immunoreactivity in the diabetic rat retina and ciliary body significantly increases compared to non-diabetic rats.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: Aquaporins are membrane water channels that play critical roles in controlling the water contents of cells. Diabetic retinopathy is associated with alterations in aquaporin water channel expression. We aimed to investigate the distribution of aquaporin 1 in non diabetic and diabetic rat retina and ciliary body. Material and Method: Eyeballs from diabetic and non diabetic rats were examined by immunohistochemistry. H-Score analysis was used for the evaluation of immunohistochemistry. Results: Aquaporin 1 immunoreactivity was found in the ganglionic cell layer (GCL), inner plexiform layer (IPL), the inner nuclear layer (INL), inner segment (IS) of photoreceptor layer (PRL) and retinal pigment epithelium (RPE) in diabetic and non diabetic rat retina and significantly increased in the diabetic rat retinas. In addition, epithelial cells of ciliary bodies were immunpositive for Aquaporin 1. No significant alterations were observed in the retinal morphology in the diabetic rats compared to non-diabetic rats. However, the demarcation between the INL and ONL was not noticed in diabetic retinas. Discussion: Our results indicate that Aquaporin 1 immunoreactivity in the diabetic rat retina and ciliary body significantly increases compared to non-diabetic rats. These results suggest that an increased expression of Aquaporin 1 in the diabetic rat retina and ciliary body may be one of the reason for retinopathy

Özet

Amaç: Aquaporinler, hücrelerin su içeriğini kontrol etmede kritik rol oynayan su kanallarıdır. Diabetik retinopati, aquaporin su kanallarının ekspresyonun-daki değişikliklerle ilişkilendirilir. Biz bu çalışmada, diabetik ve non-diabetik ratların retina ve siliyer cisminde aquaporin-1 dağılımını araştırmayı hedef-ledik. Gereç ve Yöntem: Diabetik ve non-diabetik ratların retina ve siliyer ci-simleri immunohistokimyasal olarak incelendi. İmmunohistokimyasal değer-lendirme için H-SCORE analizi kullanıldı. Bulgular: Aquaporin-1 immünoreak-tivitesi, diabetik ve non-diabetik rat retinalarının gangliyon hücre tabakasın-da (GHT), iç nükleer tabakada (INT), iç pleksiform tabakada (IPT), iç segment (IS) fotoreseptör tabakasında (FT) ve retina pigment epitelinde (RPE) bulun-du ve özellikle diabetik rat retinalarında belirgin bir artış tesbit edildi. Bu bul-gulara ilaveten siliyer cisim epitel hücrelerinde positif immunoreaktivite gö-rüldü. Diabetik ve diabetik olmayan sıçan retinaları arasında morfolojik ola-rak önemli bir değişiklik gözlenmedi. Bununla birlikte, iç nükleer tabaka (INT) ve dış nüklear tabaka (DNL) tabakaları arasındaki ayrım, diabetik sıçan reti-nalarında ortadan kalkmıştı. Sonuç: Bizim sonuçlarımız, aquaporin-1 immu-noreaktivitesinin diabetik sıçan retinası ve siliyer cisminde, diabetik olma-yan sıçanlara göre önemli şekilde arttığını gösterdi. Bu bulgulara göre diabe-tik sıçan retina ve siliyer cismindeki aquaporin-1 proteinin artışı, retinopati-nin bir sebebi olabilir.

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Cite This Article

SÖĞÜT, E., ORTAK, H., ÇAYLİ, S., TAŞ, U., EKİCİ, F., ÇAKIL, D. (2022). Diabetik sıçan retina ve siliyer cisiminde aquaporin-1 lokalizasyonu. Journal of Clinical and Analytical Medicine, Cilt: 3 - Sayı: 4, 438–441. https://ajindex.com/articles/561607

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References (14)

  1. Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004
  2. 27(5):1047- 53 2. Verkman AS. Role of aquaporin water channels in eye function. Exp Eye Res 2003
  3. 76(2):137-43. 3. Stamer WD, Snyder RW, Smith BL, Agre P, Regan JW. Localization of aquaporin CHIP in the human eye: implications in the pathogenesis of glaucoma and other disorders of ocular fluid balance. Invest Ophthalmol Vis Sci 1994
  4. 35(11):3867-72. 4. Zhang D, Vetrivel L, Verkman AS. Aquaporin deletion in mice reduces intraocular pressure and aqueous fluid production. J Gen Physiol 2002
  5. 119(6):561-9. 5. Ruiz-Ederra J, Verkman AS. Aquaporin-1 independent microvessel proliferation in a neonatal mouse model of oxygen-induced retinopathy. Invest Ophthalmol Vis Sci 2007
  6. 48(10):4802-10. 6. Cayli S, Ocakli S, Erdemir F, Tas U, Aslan H, Yener T, Karaca Z. Developmental expression of p97/VCP (Valosin-containing protein) and Jab1/CSN5 in the rat testis and epididymis. Reprod Biol Endocrinol 2011
  7. Joussen AM, Poulaki V, Le ML, Koizumi K, Esser C, Janicki H et al. A central role for inflammation in the pathogenesis of diabetic retinopathy. FASEB J 2004
  8. 18(12):1450-2. 8. Xun W, Liu Y, Qing G, Xun X, Dongqing Z, Haixiang W. Aquaporin 1 expression in retinal neovascularization in a mouse model of retinopathy of prematurity. Prep Biochem Biotechnol 2009
  9. 39(2):208-17. 9. Fukuda M, Nakanishi Y, Fuse M, Yokoi N, Hamada Y, Fukagawa M, Negi A et al. Altered expression of aquaporins 1 and 4 coincides with neurodegenerative events in retinas of spontaneously diabetic Torii rats. Exp Eye Res 2010
  10. 90(1):17-25. 10. Yang B, Folkesson HG, Yang J, Matthay MA, Ma T, Verkman AS. Reduced osmotic water permeability of the peritoneal barrier in aquaporin-1 knockout mice. Am J Physiol 1999
  11. 276(1 Pt 1):C76-81. 11. Verkman AS, Ruiz-Ederra J, Levin MH. Functions of aquaporins in the eye. Prog Retin Eye Res 2008 Jul
  12. 27(4):420-33. 12. Iandiev I, Pannicke T, Biedermann B, Wiedemann P, Reichenbach A, Bringmann A. Ischemia-reperfusion alters the immunolocalization of glial aquaporins in rat retina. Neurosci Lett 2006
  13. 408(2):108-12. 13. Oshio K, Binder DK, Bollen A, Verkman AS, Berger MS, Manley GT Aquaporin-1 expression in human glial tumors suggests a potential novel therapeutic target for tumor-associated edema. Acta Neurochir Suppl 2003
  14. Oshio K, Song Y, Verkman AS, Manley GT. Aquaporin-1 deletion reduces osmotic water perm