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Journals / International Journal of Secondary Metabolite / 2019 / Cilt: 6 - Sayı: 4

Antioxidant, Antibacterial and Antifungal Activities of Different Extracts of Silybum marianum Collected from Duhok (Iraq)

Mustafa PEHLİVAN
Falah Saleh Mohammed
Mustafa SEVİNDİK
Pages
317–322
Publication Date
2022-10-19
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was found to be effective against Candida species at 400-800 µg/mL concentrations.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, antioxidant, antibacterial and antifungal activities of Silybum marianum (L.) Gaertn. collected from Duhok (Iraq) were determined.
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Key Findings & Data:Antibacterial activity was determined against 6 bacterial strains (Staphylococcus aureus, S. aureus MRSA, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii).
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Conclusion & Impact:It was found to be effective against Candida species at 400-800 µg/mL concentrations.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Antioxidant · Antibacterial · Antifungal

Abstract

In this study, antioxidant, antibacterial and antifungal activities of Silybum marianum (L.) Gaertn. collected from Duhok (Iraq) were determined. Ethanol, methanol and dichloromethane extracts of the fruit part of plant were obtained. Antioxidant potential was determined with TAS and TOS kits using ethanol extracts. Antibacterial and antifungal activity were determined using agar dilution method. Antibacterial activity was determined against 6 bacterial strains (Staphylococcus aureus, S. aureus MRSA, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii). Antifungal activity was determined against Candida albicans, C. krusei and C. glabrata. As a result of the studies, it was determined that plant extracts have high antioxidant activity. It was also found to be effective against bacteria at 25-400 µg/mL concentrations. Plant extracts were found to be more effective against gram negative bacteria. It was found to be effective against Candida species at 400-800 µg/mL concentrations. As a result, it was determined that the fruit parts of plant could be a natural antioxidant and antibacterial source.

Özet

In this study, antioxidant, antibacterial and antifungal activities of Silybum marianum (L.) Gaertn. collected from Duhok (Iraq) were determined. Ethanol, methanol and dichloromethane extracts of the fruit part of plant were obtained. Antioxidant potential was determined with TAS and TOS kits using ethanol extracts. Antibacterial and antifungal activity were determined using agar dilution method. Antibacterial activity was determined against 6 bacterial strains (Staphylococcus aureus, S. aureus MRSA, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii). Antifungal activity was determined against Candida albicans, C. krusei and C. glabrata. As a result of the studies, it was determined that plant extracts have high antioxidant activity. It was also found to be effective against bacteria at 25-400 µg/mL concentrations. Plant extracts were found to be more effective against gram negative bacteria. It was found to be effective against Candida species at 400-800 µg/mL concentrations. As a result, it was determined that the fruit parts of plant could be a natural antioxidant and antibacterial source.

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Cite This Article

PEHLİVAN, M., Mohammed, F. S., SEVİNDİK, M. (2022). Antioxidant, Antibacterial and Antifungal Activities of Different Extracts of Silybum marianum Collected from Duhok (Iraq). International Journal of Secondary Metabolite, Cilt: 6 - Sayı: 4, 317–322. https://ajindex.com/articles/541667

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References (1)

  1. . Dar, R.A., Shahnawaz, M., Qazi, P.H. (2017). General overview of medicinal plants: A review. J. Pharmacol., 6(6), 349-351. [2]. Al–Douri, N.A. (2014). Some important medicinal plants in Iraq. Int. J. Adv. Herb. Altern. Med., 2, 10-20. [3]. Katar, D., Yaman, H., Subașı, İ., Arslan, Y. (2013). Determination of some characteristics of M1 seedling of milk thistle (Silybum marianum (L.) Gaertn.) obtained by treatment of different doses of gama irradiation. SDÜ Ziraat Fak. Derg., 8(1), 78-83. [4]. Zhao, F., Li, X.H. (2015). Evaluation of Immunomodulatory Activity of Silymarin Extract from Silybum Marianum in Mice of Health Food. Adv. J. Food Sci. Technol., 8(4), 278-282. [5]. Surai, P.F. (2015). Silymarin as a natural antioxidant: an overview of the current evidence and perspectives. Antioxidants, 4(1), 204-247. [6]. Alhidary, I.A., Rehman, Z., Khan, R.U., Tahir, M. (2017). Anti-aflatoxin activities of milk thistle (Silybum marianum) in broiler. Worlds Poult. Sci. J., 73(3), 559-566. [7]. Yue, J., Zhu, Z., Li, X. (2017). Antibacterial activity of protease hydrolysates isolated from Silybum marianum. Curr. Sci., 113(3), 00113891 [8]. Zhu, S.Y., Jiang, N., Jie, T.U., Yang, J., Zhou, Y. (2017). Antioxidant and Anti-aging Activities of Silybum Marianum Protein Hydrolysate in Mice Treated with D-galactose. Biomed Environ Sci, 30(9), 623-631. [9]. Townsend, C.C., Guest, E. (1966-1985). Flora of Iraq. vols. 1-9. Ministry of Agriculture and Agrarian Reform, Baghdad. [10]. Erel, O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. biochem., 37(4), 277-285. [11]. Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clin. Biochem., 38(12), 1103-1111. [12]. Bauer, A.W., Kirby, W.M., Sherris, J.C., Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method, Am. J. Clin. Pathol., 45, 493-96. [13]. Hindler, J., Hochstein, L.,, Howell, A. (1992). Preparation of routine media and reagents used in antimicrobial susceptibility testing. Part 1. McFarland standards, 5.19.1-5.19.6. In H. D. Isenberg (ed) Clinical microbiology procedures handbook, vol. 1. American Society for Microbiology, Washington, D.C. [14]. CLSI (The Clinical and Laboratory Standards Institute). (2012). Antimicrobial Susceptibility Testing of Anaerobic Bacteria; Approved Standard-Eighth Edition (M11-A8). [15]. EUCAST (European Committee on Antimicr