SavedAdd your journal

Journals / International Advanced Researches and Engineering Journal / 2019 / Cilt: 3 - Sayı: 2

Electrophoretic characterization of inbred maize lines

Burak UYANIK
Kayıhan Z. KORKUT
Yaren MENGİ
Görkem DALGIÇ
Elif Eymen ÖZDEMİR
Gülsemin SAVAŞ TUNA
Pages
86–92
Publication Date
2022-10-19
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was found out that the relative mobility was minimum in the alpha region.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, 50 inbred lines (S4) of maize (Zea mays indentata Sturt.), which were developed by the Department of Field Crops, Faculty of Agriculture, University of Namık Kemal, were used as a material.
📊
Key Findings & Data:At the end of the electrophoresis examinations, it was revealed that the band number of the gliadin proteins in the inbred maize lines was between 11 and 20, the relative mobility of the genotypes was between 18 and 90 kDA, and according to the gliadin regions, the bands were mainly in the omega, beta and gamma regions, respectively.
💡
Conclusion & Impact:It was found out that the relative mobility was minimum in the alpha region.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Zea mays L. · Inbred line · SDS-PAGE

Abstract

In this study, 50 inbred lines (S4) of maize (Zea mays indentata Sturt.), which were developed by the Department of Field Crops, Faculty of Agriculture, University of Namık Kemal, were used as a material. In the study, the band patterns of the gliadin protein of inbred lines were determined by the SDS – PAGE method, and regarding the ratio density data of the genotypes, the number of the bands and the spreading of them to the gliadin regions were examined. At the end of the electrophoresis examinations, it was revealed that the band number of the gliadin proteins in the inbred maize lines was between 11 and 20, the relative mobility of the genotypes was between 18 and 90 kDA, and according to the gliadin regions, the bands were mainly in the omega, beta and gamma regions, respectively. It was found out that the relative mobility was minimum in the alpha region. As a result of the study, it was determined that some lines were formed by similar populations with the obtained band patterns, and the majority were different. This indicates that genetic diversity exists in the examined lines, and the obtained data can be used in the breeding studies.

🔖

Cite This Article

UYANIK, B., KORKUT, K. Z., MENGİ, Y., DALGIÇ, G., ÖZDEMİR, E. E., TUNA, G. S. (2022). Electrophoretic characterization of inbred maize lines. International Advanced Researches and Engineering Journal, Cilt: 3 - Sayı: 2, 86–92. https://ajindex.com/articles/539358

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Ranere, A.J., Piperno, D.R., Holst, I., Dickau, R., Iriarte, J. The cultural and chronological context of early Holocene maize and squash domestication in the Central Balsas River Valley, Mexico. Proc Natl Acad Sci, 2009. 106: 5014–5018
  2. FAO Internet Web Site, FAOSTAT. Database. [cited 2017 1 August]; Available from: www.fao.org 3. Ziraat Mühendisleri Odası, [cited 2016 10 August]; Available from: http://www.zmo.org.tr/genel 4
  3. Cengiz, R. Türkiye’de Kamu Mısır Araştırmaları [Public Maize Investigations in Turkey]. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, 2016. 25: 304-310
  4. USDA/FAS, cited 2015 21 January]; Available from:
  5. Pingali, P.L. CIMMYT 1999-2000, World Maize Facts and Trends. Meeting World Maize Needs: Technological Opportunities and Priorities for the Public Sector. 2001. Mexico, D.F.: CIMMYT
  6. Osborne, T. B., The amount and properties of the proteids of the maize kernel. J. Amer. Chem. Soc, 1897. 19: 525-532
  7. Dierks-Ventling, C., Cozens, K., Immunochemical Crossreactivity Between Zein, Hordein And Gliadin. Herbs Letters, 1982. 14:147-150
  8. Valentini, G., Soave, C., Ottaviano, E., Chromosomal location of zein genes in Zea mays. Heredity, 1979. 42:33–40
  9. Esen, A., A proposed nomenclature for the alcohol-soluble proteins (zeins) of maize (Zea mays L.). Journal of Cereal Science, 1987. 5:117-128
  10. Holding, D.R., Larkins, B.A. Zein storage proteins. In Molecular Genetic Approaches to Maize Improvement, AL KrizBA Larkins, Biotechnology in Agriculture and Forestry, Springer- Verlag Berlin Heidelberg, Germany, 2009. 63: 269–286
  11. Krishnan, H.B., Kerley, M.S., Allee, G.L., Jang, S., Kim, W.S. et al. Maize 27 kDa γ-Zein Is a Potential Allergen for Early Weaned Pigs. J Agric Food Chem, 2010. 58: 7323–7328. 13. Șuteu, D., Băcilă, I., Haș,V., Haș, I., Miclăuș, I., Romanian Maize (Zea mays) Inbred Lines as a Source of Genetic Diversity in SE Europe, and Their Potential in Future Breeding Efforts. Plos One, 2013. 8:1-13
  12. Lookhart, G. and Bean, S., Separation and characterization of wheat protein fractions by high-performance capillary electrophoresis. Cereal Chem., 1995. 72: 527–532. 15. Đukić, N., Matić, G., Konjević, R. Biochemical Analysis Of Gliadins Of Wheat Triticum. Kragujevac J. Sci., 2005. 27:131-138
  13. Waga, J. and Zientarski, J., Isolation And Purification Of Individual Gliadin Proteins by Preparative Acid Polyacrylamide Gel Electrophoresis (A-Page). Poli