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Journals / International Advanced Researches and Engineering Journal / 2021 / Cilt: 5 - Sayı: 1

Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer

Rizvan İMAMOĞLU
İsa GÖKÇE
Özlem KAPLAN
İskender ŞAHİNGÖZ
Pages
42–46
Publication Date
2022-10-19
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was shown by the PCR experiment that the SSB protein produced in this study could increase the amplification efficiency.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Single-stranded DNA-binding (SSB) proteins play an important role in DNA metabolism involving DNA replication, recombination, and repair in all living beings.
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Key Findings & Data:In molecular biology, SSB proteins are used as enhancers to increase the efficiency and specificity of PCR.
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Conclusion & Impact:It was shown by the PCR experiment that the SSB protein produced in this study could increase the amplification efficiency.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Polymerase Chain Reaction · Single Strand Binding Protein · Thermus aquaticus

Abstract

Single-stranded DNA-binding (SSB) proteins play an important role in DNA metabolism involving DNA replication, recombination, and repair in all living beings. In molecular biology, SSB proteins are used as enhancers to increase the efficiency and specificity of PCR. Thermostable SSB protein eliminates secondary structure or dimer formation and significantly increase the effectiveness of amplification of DNA fragments. In this study, it was ensured that the SSB gene of thermophilic bacteria Thermus aquaticus (T. aquaticus) was cloned into the pET28b vector and expressed in E. coli BL21 (DE3) PLysE cells. Then, the purification of the SSB protein produced in E. coli BL21 (DE3) PLysE cells was performed. 20 mg SSB protein was obtained from 1L bacterial culture, and its purity was more than 90%. It was shown by the PCR experiment that the SSB protein produced in this study could increase the amplification efficiency.

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Cite This Article

İMAMOĞLU, R., GÖKÇE, İ., KAPLAN, Ö., ŞAHİNGÖZ, İ. (2022). Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer. International Advanced Researches and Engineering Journal, Cilt: 5 - Sayı: 1, 42–46. https://ajindex.com/articles/539285

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