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Journals / Hacettepe Journal of Biology and Chemistry / 2007 / Cilt: 35 - Sayı: 2

In vitro shoot regeneration of oregano (origanum minutiflorum o.schwarz & davis)

Dudu ÖZKUM
Pages
97–100
Publication Date
2022-10-17
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However for all explants, B5 medium with all phytohormone combinations had no effective success and therefore were not suitable on in vitro shoot regeneration.

📌 3 Key Takeaways

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Objective & Hypothesis:In vitro shoot regeneration procedure for oregano (O. minutiflorum O.
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Key Findings & Data:Leaf segments and shoot explants (hypocotyl, single nodal segment and shoot tip) excised from 30-40 d old in vitro germinated seedlings and cultured on Murashige and Skoog (MS) and Gamborg (B5) media containing different combinations of benzyl amino purine (BAP) (0.0, 1.0, 2.0 or 3.0 mg L-1) and naphtalene acetic acid (NAA) (0.0, 0.1 or 0.5 mg L-1).
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Conclusion & Impact:However for all explants, B5 medium with all phytohormone combinations had no effective success and therefore were not suitable on in vitro shoot regeneration.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In vitro shoot regeneration procedure for oregano (O. minutiflorum O. Schwarz & Davis), endemic species of Turkey, is described. Leaf segments and shoot explants (hypocotyl, single nodal segment and shoot tip) excised from 30-40 d old in vitro germinated seedlings and cultured on Murashige and Skoog (MS) and Gamborg (B5) media containing different combinations of benzyl amino purine (BAP) (0.0, 1.0, 2.0 or 3.0 mg L-1) and naphtalene acetic acid (NAA) (0.0, 0.1 or 0.5 mg L-1). The single nodal segments were the most succesful explant in all hormone combinations used. MS medium supplemented with 2.0 mg. L-1 BAP + 0.1 mg L-1 NAA was the most effective medium for shoot formation. However for all explants, B5 medium with all phytohormone combinations had no effective success and therefore were not suitable on in vitro shoot regeneration.

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Cite This Article

ÖZKUM, D. (2022). In vitro shoot regeneration of oregano (origanum minutiflorum o.schwarz & davis). Hacettepe Journal of Biology and Chemistry, Cilt: 35 - Sayı: 2, 97–100. https://ajindex.com/articles/529389

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