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Journals / Hacettepe Journal of Biology and Chemistry / 2008 / Cilt: 36 - Sayı: 1

Synthesis and characterization of macroporous poly(acrylamide methacrylamido histidine) cryogels and their use in antibody purification

Nilay BERELİ
Igor Y. GALAEV
Gözde BAYDEMİR
Adil DENİZLİ
İlker KOÇ
Pages
61–76
Publication Date
2022-10-17
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was observed that IgG could be repeatedly adsorbed and desorbed with poly(AAm-MAH) cryogel without significant loss in the adsorption capacity.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Poly(acrylamide-methacrylamidohistidine) [poly(AAm-MAH)] cryogel was prepared by bulk polymerization which proceeds in aqueous solution of monomers frozen inside a syringe column (cryo-polymerization).
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Key Findings & Data:After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 μm size.
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Conclusion & Impact:It was observed that IgG could be repeatedly adsorbed and desorbed with poly(AAm-MAH) cryogel without significant loss in the adsorption capacity.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Poly(acrylamide-methacrylamidohistidine) [poly(AAm-MAH)] cryogel was prepared by bulk polymerization which proceeds in aqueous solution of monomers frozen inside a syringe column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 μm size. Poly(AAm-MAH) cryogel was characterized by swelling studies, FTIR and scanning electron microscopy. The equilibrium swelling degree of the poly(AAm-MAH) monolithic cryogel was 6.97 g H2O/g cryogel. Poly(AAm-MAH) cryogel was used in the adsorption/desorption of immunoglobulin G (IgG) from aqueous solutions and human plasma. The non-specific adsorption of IgG was very low (0.22 mg/g). The maximum amount of IgG adsorption from aqueous solution in HEPES buffer was 32.879 mg/g at pH 7.4. Higher IgG adsorption value was obtained from human plasma (up to 100 mg/g). Desorption of IgG from poly(AAm-MAH) cryogel was obtained using 20 mM Acetate buffer containing 1.0 M NaCl at pH 4. It was observed that IgG could be repeatedly adsorbed and desorbed with poly(AAm-MAH) cryogel without significant loss in the adsorption capacity.

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Cite This Article

BERELİ, N., GALAEV, I. Y., BAYDEMİR, G., DENİZLİ, A., KOÇ, İ. (2022). Synthesis and characterization of macroporous poly(acrylamide methacrylamido histidine) cryogels and their use in antibody purification. Hacettepe Journal of Biology and Chemistry, Cilt: 36 - Sayı: 1, 61–76. https://ajindex.com/articles/529356

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