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Journals / Hacettepe Journal of Biology and Chemistry / 2026 / Volume: 36 Issue: 1

Supermacroporous Support Having Dye for Reuseable Enzyme Immobilization

M. Nalan Tüzmen(Dokuz Eylül University, Department of Chemistry, İzmir, Turkey)
Nilay Bereli(Hacettepe University, Department of Chemistry, Ankara, Turkey)
Müge Andaç(Hacettepe University, Department of Chemistry, Ankara, Turkey)
Sinan Akgöl(Adnan Menderes University, Department of Chemistry, Aydın, Turkey)
Adil Denizli(Hacettepe University, Department of Chemistry, Ankara, Turkey)
Pages
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Publication Date
2008-01-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

We concluded that Cibacron Blue F3GA attachedpoly AAm-AGE cryogel could be repeatedly applied forβ-casein adsorption without significant losses in the adsorptioncapacity

📌 3 Key Takeaways

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Objective & Hypothesis:Poly acrylamide-allyl glycidyl ether [poly AAm-AGE ] cryogel was prepared by bulk polymerization which proceedsin an aqueous solution of monomers frozen inside a glass column cryo-polymerization .
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Key Findings & Data:After thawing, the monolithiccryogel contains a continuous polymeric matrix having interconnected pores of 10-100 µm size.
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Conclusion & Impact:We concluded that Cibacron Blue F3GA attachedpoly AAm-AGE cryogel could be repeatedly applied forβ-casein adsorption without significant losses in the adsorptioncapacity
🔬Design:Article
📍Scope:Comprehensive Sample
⚙️Variables:β-Casein · cryogels · Dye-affinity Chromatography

Abstract

Poly acrylamide-allyl glycidyl ether [poly AAm-AGE ] cryogel was prepared by bulk polymerization which proceedsin an aqueous solution of monomers frozen inside a glass column cryo-polymerization . After thawing, the monolithiccryogel contains a continuous polymeric matrix having interconnected pores of 10-100 µm size. Cibacron Blue F3GAwas attached by covalent binding onto poly AAm-AGE cryogel via epoxy groups. Poly AAm-AGE cryogel wascharacterized by FTIR and scanning electron microscopy SEM . The equilibrium swelling degree of the poly AAmAGE monolithic cryogel was 6.84 g H2O/g cryogel. Poly AAm-AGE cryogel containing 68.9 µmol Cibacron BlueF3GA/g was used in the adsorption ofβ-casein from aqueous solution. The effects of initial concentration, pH, ionicstrength and flow rate on the adsorption efficiency of cryogel were studied in a column system. The non-specificadsorption ofβ-casein was very low 1.26 mg/g polymer . The maximum amount of β-casein adsorption from aqueoussolution in acetate buffer was 44.7 mg/g at pH 4.0. Up to 99% of the adsorbedβ-casein was eluted when 0.1 MTris/HCl buffer containing 0.5 M NaCl was used as elution agent. We concluded that Cibacron Blue F3GA attachedpoly AAm-AGE cryogel could be repeatedly applied forβ-casein adsorption without significant losses in the adsorptioncapacity

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Cite This Article

Tüzmen, M. N., Bereli, N., Andaç, M., Akgöl, S., Denizli, A. (2008). Supermacroporous Support Having Dye for Reuseable Enzyme Immobilization. Hacettepe Journal of Biology and Chemistry, Volume: 36 Issue: 1. https://ajindex.com/articles/1271145

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References (50)

  1. G. M. S. Finette, Q. M. Mao, M. T. W. Hearn, J. Chromatogr. A., 763 (1997) 71.
  2. S. Fisichella, G. Alberghina, M. E. Amato, D. Lafiandra, D.Mantarro, A. Palermo, Savarino, G.Scarlata, J. Cereal Sci., 38 (2003) 77.
  3. M.Grasselli, S. A. Camperi, d A. A. N.el Canizo, O.Cascone, J.Sci. Food and Agricul., 79 (1999) 333.
  4. S. Zhang, Y. Sun, Biotechnol. Prog., 20 (2004) 207.
  5. E. P. Lillehoj, V. S. Malik, in: Advances in Biochemical Engineering/Biotechnology, A. Fiechter, Ed., Springer-Verlag, Berlin 1982, pp. 20– 65.
  6. Y. Arica, H. N. Testereci, A. Denizli, J. Chromatogr. A, 799 (1998) 83.
  7. S. Akgol, D. Turkmen, A. Denizli, J. Appl. Polym. Sci., 93 (2004) 2669.
  8. S. Senel, S. Akgol,Y. Arica, A. Denizli, Polym. Int., 50 (2001) 1143.
  9. S. Akgol, A. Denizli, J. Mol. Cat. B: Enzym., 28 (2004) 7.
  10. M. Odabasi, A. Denizli, Polym. Int., 53 (2004) 332.
  11. H. Yavuz, A. Denizli, Macromol. Biosci., 5 (2005) 39.
  12. H. Yavuz, A. Denizli, Macromol. Biosci., 4 (2004) 84.
  13. R.M. Birch, C. O’Byrne, I.R. Booth, P. Cash, Proteomics, 3 (2003) 764.
  14. A. Denizli, E. Pişkin, J. Biochem. Biophys. Methods, 49 (2001) 391.
  15. M. Odabasi, A. Denizli, Polym. Int., 53 (2004) 332.
  16. S. Zhang, Y. Sun, Ind. Eng. Chem. Res., 42 (2003) 1235.
  17. M. Zhang, Y. Sun, Biotechnol. Bioeng., 75 (2001) 710.
  18. M. Zhang, Y. Sun, J. Chromatogr. A., 912 (2001) 31.
  19. H. Yavuz, S. Akgöl, R. Say, A. Denizli, Int.J. Biol. Macromol., 39 (2006) 303.
  20. S. Akgöl, A. Denizli, J. Molecular Catalyis B., 28 (2004) 7.
  21. M. McCoy, K. Kalghatgi, F.E. Regnier, N. Afeyan, J. Chromatogr. A., 743 (1996) 221.
  22. E.B. Altıntas, A. Denizli, J. Appl. Polym. Sci., 103 (2007) 975.
  23. S. Özkara, B. Garipcan, E. Piflkin, A. Denizli, J. Biomater. Sci. Polym. Ed., 14 (2003) 761.
  24. V.I. Lozinsky, I.Y. Galaev, F.M. Plieva, I.N. Savina, H. Jungvid, B. Mattiason, Trends in Biotechnol., 21 (2003) 445.
  25. P. Arvidsson, F.M. Plieva, V.I. Lozinsky, I.Y. Galaev, B. Mattiasson, J. Chromatogr. A., 986 (2003) 275.
  26. V.I. Lozinsky, F.M. Plieva, I. Y. Galaev, B. Mattiasson, Bioseparation, 10 (2002) 163.
  27. P. Arvidsson, F.M. Plieva, I.N. Savina, V.I. Lozinsky, S. Fexby, L. Bülow, I.Y. Galaev, B. Mattiasson, J. Chromatogr. A., 977 (2002) 27.
  28. C. Babaç, H. Yavuz, I.Y. Galaev, E. Piflkin, A. Denizli, React. Functl. Polym., 66 (2006) 1263.
  29. M.B. Dainiak, I.Y. Galaev, B. Matiasson, J. Chromatogr. A., 1123 (2006) 145.
  30. I.M. Savina, F.Plieva, V.A. Izumrudov, B. Mattiasson, I. Y. Galaev, J. Biotechnol., 123 (2006) 209.
  31. N. Demiryas, N. Tüzmen, I.Y. Galaev, E. Pişkin, A. Denizli, J. Appl. Polym. Sci., in press.
  32. C. G. De Kruif, V. Y. Grinberg, Coll. Surf. A., 210 (2002) 183.
  33. K. Landström, T. Arnebrant, J. Alsins, B. Bergenstahl, Food Hydrocolloids, 17 (2003), 103- 116.
  34. C. G. De Kruif, in: Industrial Proteins,W. Aalbersberg, Ed., Elsevier Sci., London 2003, Chapter 6.
  35. E. Dickinson, S. Krishna, Food Hydrocolloids, 15 (2001) 107-115.
  36. M. R. R. Nino, C. C. Sanchez, J. M. R. Patino, Coll. Surf. B., 12 (1999) 161.
  37. H. E. Swaisgood, Adv. Dairy Chem., 1 (1992) 63.
  38. S. Akgöl, N. Bereli, A. Denizli, Macromol. Biosci. 5 (2005), 786-794.
  39. Y. Igarashi, Int. Dairy J., 9 (1999) 377.
  40. P. Arvidsson, F.M. Plieva, I.N. Savina, V.I. Lozinsky, S. Fexby, L. Bülow, I.Y. Galaev, B. Mattiasson, J. Chromatogr. A., 977 (2002) 27.
  41. B. Weber, K. Willeford, J. Moe, D. Piskiewicz, Biochem. Biophys. Res. Commun., 86 (1979) 252.
  42. C.V. Stead, in: Y.D. Clonis, A. Atkinson, C.J. Bruton, C.R. Lowe (Eds.), Reactive Dyes in Protein and Enzyme Technology, Macmillan, Basingstoke, 1987, p. 13.
  43. P.M. Boyer, J.T. Hsu, Adv. Biochem. Eng., 49 (1993) 1.
  44. C.R. Lowe, in: A. Wiseman (Ed.), Topics in Enzyme and Fermentation Technology, Ellis Horwood, Chichester 1984, p. 78.
  45. X. Zhu, S.D. Alexandratos, Ind. Eng. Chem. Res., 44 (2005) 8605.
  46. Y.S. Ho, G. McKay, Proc. Biochem., 34 (1999) 451.
  47. L. Uzun, A. Denizli, J. Biomater. Sci. Polym. Ed., 17 (2006) 791.
  48. S.J. Allen, B. Koumanova, Z. Kircheva, S. Nenkova, Ind. Eng. Chem. Res., 44 (2005) 2281.
  49. S. Oncel, L. Uzun, B. Garipcan, A. Denizli, Ind. Eng. Chem. Res., 44 (2005) 7049.
  50. Y. Arica, H. N. Testereci, A. Denizli, J. Chromatogr. A, 799 (1998) 83.