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Journals / Hacettepe Journal of Biology and Chemistry / 2009 / Cilt: 37 - Sayı: 4

Effect of some chemicals on G6PD enzyme purified by affinity chromatography

İsmail ÖZMEN
Pages
327–330
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These three chemicals promoted the enzyme activity.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzyme defect, being present in more than 400 million people worldwide.
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Key Findings & Data:The clinical manifestations of G6PD deficiency are neonatal jaundice, and acute haemolytic anaemia, which is usually triggered by an exogenous agent.
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Conclusion & Impact:These three chemicals promoted the enzyme activity.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzyme defect, being present in more than 400 million people worldwide. The clinical manifestations of G6PD deficiency are neonatal jaundice, and acute haemolytic anaemia, which is usually triggered by an exogenous agent. The aim of this study was to investigate the enhancing effect of some chemicals on G6PD. G6PD was purified from human erythrocyte by using ammonium sulphate precipitation and 2´,5´-ADP-Sepharose 4B affinity gel. The purified enzyme showed a single band on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). The effects of three different chemicals (theophylline ethylenediamine, pentoxifyllin, thiamphenicol glycinate hydrochloride) were investigated on the purified enzyme. These three chemicals promoted the enzyme activity.

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Cite This Article

ÖZMEN, İ. (2022). Effect of some chemicals on G6PD enzyme purified by affinity chromatography. Hacettepe Journal of Biology and Chemistry, Cilt: 37 - Sayı: 4, 327–330. https://ajindex.com/articles/529329

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References (2)

  1. Mates, J.M., Perez-Gomez, C., Nunez De Castro, I. Antioxidant Enzymes and Human Diseases. Clinical Biochem.; 32 8, 595-603, 1999. 2.Cappellini, M.D., Fiorelli, G., Glucose-6-phosphate dehydrogenase deficiency. Lancet, 371, 64-74, 2008. 3.Gupte, R.S., Floyd, B.C., Kozicky, M., George, S., Ungvari, Z.I., Neito, V., Wolin, M.S., Gupte, S.A. Free Rad. Biol. Med., 47, 219-228, 2009. 4.McCord, J.M. Human disease, free radicals, and the oxidant/antioxidant balance. Clin. Biochem., 26, 351-357 1993. 5.Jenkins, R.R., Goldfarb, A. Introduction: oxidant stress aging and exercise. Med. Sci. Sports Exer., 25, 210-212 1993. 6.Bondy, S.C., Orozco, J. Effects of ethanol treatment upon sources of reactive oxygen species in brain and liver. Alcohol, 29, 375-83, 1994. 7.Halliwell, B., Gutteridge, J.M.C. Free Radicals in Biology and Medicine, (2nd ed.), Clarendon Press, Oxford, 1989. 8.Somani, S.M. Exercise, drugs and tissue specific antioxidant system. In: Somani, S.M. Ed. Pharmacology in Exercise and Sports, CRC Press,
  2. Boca Raton, FL. 57-95, 1996. 9.Alving, A., Carson, P., Flanagan, C.I., Ickes, C. Enzymatic deficiency in primaquine-sensitive erythrocytes. Science, 14, 484-485, 1956. 10.Ninfali, P., Orsenigo, T., Barociani, L., Rapa, S. Rapid purification of glucose-6-phosphate dehydrogenase from mammal's erythrocytes. Prep. Biochem., 20, 297-309, 1990. 11.Beutler, E. Red cell metabolism, A manual of Biochemical Methods, 3nd ed. Grune and Starton, Inc. Orlando, FL 32887, London,248-251, 1984. 12.Bradford, M.M. A rapid and sensitive method for the quantition of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-51, 1976. 13.Laemmli, D.K. Clevage of structual proteins during in assembly of the head of bacteriophage T. Nature, 4, 227 680-683, 1970. 14.Kellenmeyer, R.W., Tarlov, A.R., Brewer, G.J., Garson, P.E., Alving, A.S. Hemolytic effect of therapeutic drugs, clinical considerations of the primaqunine-type hemolysis. JAMA, 180, 388-391, 1962. 15.Ozmen, İ., Çiftçi, M., Küfrevioğlu, Ö.İ., Çürük, M.A. Investigation of Glucose 6-Phosphate Dehydrogenase (G6PD) Kinetics for Normal and G6PD-Deficient Persons and the Effects of Some Drugs. J. Enzyme Inh. Med. Chem., 19(1), 45-50, 2004. 16.Yilmaz, H.R., lraz,.M„ Soğut, S., Ozyurt, H„ Yildirim, Z„ Akyol, O., Gergerlioglu, S. The effects of erdosteine on the activities of some metabolic enzymes during cisplatin-induced nephrotoxicity in rats. Pharmacol. Res. 50(3), 287-90, 2004.