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Dergiler / Hacettepe Journal of Biology and Chemistry / 2009 / Cilt: 37 - Sayı: 4

Effect of some chemicals on G6PD enzyme purified by affinity chromatography

İsmail ÖZMEN
Sayfa
327–330
Yayın Tarihi
2022-10-17
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

These three chemicals promoted the enzyme activity.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzyme defect, being present in more than 400 million people worldwide.
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Temel Bulgular & Veri:The clinical manifestations of G6PD deficiency are neonatal jaundice, and acute haemolytic anaemia, which is usually triggered by an exogenous agent.
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Sonuç & Katkı:These three chemicals promoted the enzyme activity.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzyme defect, being present in more than 400 million people worldwide. The clinical manifestations of G6PD deficiency are neonatal jaundice, and acute haemolytic anaemia, which is usually triggered by an exogenous agent. The aim of this study was to investigate the enhancing effect of some chemicals on G6PD. G6PD was purified from human erythrocyte by using ammonium sulphate precipitation and 2´,5´-ADP-Sepharose 4B affinity gel. The purified enzyme showed a single band on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). The effects of three different chemicals (theophylline ethylenediamine, pentoxifyllin, thiamphenicol glycinate hydrochloride) were investigated on the purified enzyme. These three chemicals promoted the enzyme activity.

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Bu Makaleye Atıf Yap

ÖZMEN, İ. (2022). Effect of some chemicals on G6PD enzyme purified by affinity chromatography. Hacettepe Journal of Biology and Chemistry, Cilt: 37 - Sayı: 4, 327–330. https://ajindex.com/articles/529329

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Kaynakça (2)

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