Chromatographic hydrophobicity parameter and chromatographic hydrophobicity index were also calculated.
📌 3 Key Takeaways
🎯
Objective & Hypothesis:In this work, the proportion of organic modifier of acetonitrile–water mixtures used as mobile phases were optimized in order to separate the a-aspartame and six breakdown compounds by using the relationships between the retention parameters of the compounds and the percentage of organic modifier.
📊
Key Findings & Data:The separation was accomplished under ion pair reversed phase conditions using an YMC ODS AM (250x4.6 mm I.D., 5 μm) column.
💡
Conclusion & Impact:Chromatographic hydrophobicity parameter and chromatographic hydrophobicity index were also calculated.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters
Abstract
In this work, the proportion of organic modifier of acetonitrile–water mixtures used as mobile phases were optimized in order to separate the a-aspartame and six breakdown compounds by using the relationships between the retention parameters of the compounds and the percentage of organic modifier. The separation was accomplished under ion pair reversed phase conditions using an YMC ODS AM (250x4.6 mm I.D., 5 μm) column. Optimum mobile phase for the chromatographic separation is composed of acetonitrile-water (20:80, v/v). Concentration of heptanesulfonic acid is 2.$10^ {-3}$ M at this mobile phase and the pH of the mobile phase is 2.60. Chromatographic hydrophobicity parameter and chromatographic hydrophobicity index were also calculated.
🔖
Cite This Article
ALSANCAK, G., DEMİRALAY, E. Ç. (2022). Optimization of ion pair reversed phase liquid Chromatography separation of a-aspartame and breakdown productsalpha. Hacettepe Journal of Biology and Chemistry, Cilt: 37 - Sayı: 1, 67–75. https://ajindex.com/articles/529324
Kim, S.K., Jung, M.Y., Kin, S.Y., Photodecomposition of aspartame in aqueous solutions, Food Chem., 59, 273, 1997. 2.Prudel, M., Davıdkovâ, E., Davidek, J., Kminek, M., Kinetics of decomposition of aspartame hydrochloride (Usal) in aqueous solution, J. Food Sci., 51(6), 1393, 1986. 3.Homier, B.E., Properties and stability of aspartame, Food Technol., 38(7), 50, 1984. 4.Sabah, S., Scriba, G.K.E., Determination of aspartame and its degradation and epimerization products by capillary electrophoresis, J. Pharm. Biomed. Anal., 16, 1089,1998. 5.Stenberg, M., Marko-Varga, G., Oste, R., Racemization of amino acids during classical and microwave oven hydrolysis - application to aspartame and a Maillard reaction system, Food Chem., 74, 217, 2001. 6.Hsieh T.J., Chen, S., A facile HPLC method for optical purity and quantitative measurements of phenylalanine from the hydrolyzed aspartame under different pH and temperature after its derivatization with a fluorescent reagent, Amino Acids, 33, 123, 2007. 7.Bell, L.N., Wetzel, C.R., Aspartame degradation in solution as impacted by buffer type and concentration, J. Agric. Food Chem., 43, 2608, 1995. 8.Lawrence, J.F., Iyengar, J.R., Liquid chromatographic determination of beta-aspartame in diet soft drinks, beverage powders and pudding mixes, J. Chromatogr., 404,261,1987. 9.Verzella, G., Bagnasco, G. and Mangia, A., Ion-pair high-performance liquid chromatographic analysis of aspartame and related products, J. Chromatogr., 849, 83, 1985. 10.Gaines, S.M., Bada, J.L., Reversed-phase high-performance liquid chromatographic separation of aspartame diastereomeric decomposition products, J. Chromatogr., 389, 219, 1987. 11.Tsang, W.S., Clarke, M.A., Parrish, F.W., Determination of aspartame and its breakdown products in soft drinks by reverse-phase chromatography with UV detection, J. Agric. Food Chem., 33, 734,1985. 12.Stamp, J.A., Labuza, T.P., An ion-pair high performance liquid chromatographic method for the determination of aspartame and its decomposition products, J. Food Sci., 54(4), 1043, 1989. 13.Demiralay, E.C., Özkan, G., Optimization strategy for isocratic separation of a-aspartame and its breakdown products by reversed phase liquid chromatography, Chromatographia, 60 (9/10), 579, 2004. 14.Rondinini, S., Mussini, P.R., Mussini, T., Reference value standards and primary standards for pH measurements in organic solvents and water+organic solvent mixtures of moderate to high permittivities, Pure Appl. Chem., 59, 1549, 19