SavedAdd your journal

Journals / Hacettepe Journal of Biology and Chemistry / 2011 / Cilt: 39 - Sayı: 2

Bazı alfa-karbonik anhidrazlarda oksitetrasiklinin aktivatör etkileri

Activatory effects of oxytetracycline on some α-carbonic anhydrases

Ömer İrfan KÜFREVİOĞLU
Murat ŞENTÜRK
Ramazan DEMİRDAĞ
Pages
207–212
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Activity % values for this antibiotic were determined by plotting activity % vs. [A].

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Three μ-carbonic anhydrases, including human carbonic anhydrase I and II (hCA I, II) and sheep kidney carbonic anhydrase (sCA) were purified and kinetic interactions between the enzymes and oxytetracycline were investigated.
📊
Key Findings & Data:The purification procedure was composed of preparation of homogenate (or hemolysate) and affinity chromatography on Sepharose 4B-tyrosine-sulfanilamide.
💡
Conclusion & Impact:Activity % values for this antibiotic were determined by plotting activity % vs. [A].
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Three μ-carbonic anhydrases, including human carbonic anhydrase I and II (hCA I, II) and sheep kidney carbonic anhydrase (sCA) were purified and kinetic interactions between the enzymes and oxytetracycline were investigated. The purification procedure was composed of preparation of homogenate (or hemolysate) and affinity chromatography on Sepharose 4B-tyrosine-sulfanilamide. Oxytetracycline exhibited in vitro activatory effects on hCA I, II and sCA enzymes activity. Activity % values for this antibiotic were determined by plotting activity % vs. [A].

Özet

Üç alfa-karbonik anhidraz, insan karbonik anhidraz I, II (hCA I, II) ve koyun böbrek karbonik anhidraz (sCA) saflaştırıldı ve enzimlerle oksitetrasiklinin kinetik etkileşmeleri incelendi. Saflaştırma prosedürü homojenat hazırlama ve Sepharose 4B-trizonsüllfanilamid afinite kramatografisinden oluştu. Oksitetrasiklin hCA I, II ve sCA enzim aktivitelerinde in vitro aktivatör etkisi gösterdi. Bu antibiyotik için % aktivite-[A] grafiği kullanılarak % aktivite değerleri belirlendi.

🔖

Cite This Article

KÜFREVİOĞLU, Ö. İ., ŞENTÜRK, M., DEMİRDAĞ, R. (2022). Bazı alfa-karbonik anhidrazlarda oksitetrasiklinin aktivatör etkileri. Hacettepe Journal of Biology and Chemistry, Cilt: 39 - Sayı: 2, 207–212. https://ajindex.com/articles/529256

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. R. Parui, K.K. Gambir, P.P. Mehrotra, Changes in carbonic anhydrase may be the initial step of altered metabolism in hypertension, Biochem. Int., 23(1991) 779
  2. C.T. Supuran, A. Scozzafava, Carbonic anhydrase inhibitors and their therapeutic potential. Exp. Opin. Ther. Patents, 10 (2000) 575
  3. D. Hewett-Emmett, Evolution and distribution of the carbonic anhydrase gene families. In: W.R. Chegwidden, N.D. Carter, Y.H. Edwards (Eds). The Carbonic Anhydrases. New Horizons. Basel: Birkhauser Verlag, (2000) pp 29
  4. T. Stams, D.W. Christianson, X-ray crystallographic studies of mammalian carbonic anhydrase isozymes. In: W.R. Chegwidden, N.D. Carter, Y.H. Edwards, (Eds.), The Carbonic Anhydrases: New Horizons. Birkhauser Verlag, Boston (2000) pp. 159
  5. F. Briganti, A. Scozzafava, C.T. Supuran, Novel carbonic anhydrase isozymes I, II, and IV activators incorporating sulfonylhistamino moieties. Bioorg. Med. Chem. Lett, 9 (1999) 2043
  6. F. Briganti, V. Iaconi, S. Mangani, P. Orioli, A. Scozzafava, G. Vernaglione, C.T. Supuran, Carbonic anhydrase activators: the X-ray crystallographic characterization of the ternary complex of phenylalanine and azide with human isozyme II. lnorg. Chim. Acta, 275 (1998) 295
  7. B.W. Clare, C.T. Supuran, Carbonic anhydrase activators. 3: structure-activity correlations for a series of isozyme II activators. J. Pharm. Sci, 83 (1994) 768
  8. C.T. Supuran, A.T. Balaban, P. Cabildo, R.M. Claramunt, J.L. Lavandera, J. Elguero, Carbonic anhydrase activators. VII. Isozyme II activation by bisazolylmethanes, -ethanes and related azoles. Biol. Pharm. Bull, 16 (1993) 1236
  9. E. Bayram, M. Senturk, O.I. Kufrevioglu, C.T. Supuran, In vitro inhibition of salicylic acid derivatives on human cytosolic carbonic anhydrase isozymes I and II, Bioorg. Med. Chem, 16 (2008) 9101
  10. S.B. Ceyhun, M. Senturk, O. Erdogan, O.I. Kufrevioglu, In vitro and in vivo effects of some pesticides on carbonic anhydrase enzyme from rainbow trout (Oncorhynchus mykiss) gills. Pesticide Biochem. Physiol, 97 (2010) 177
  11. K.M. Wilbur, N.G. Anderson, Electrometric and colorimetric determination of carbonic anhydrase J. Biol. Chem, 176 (1948) 147
  12. J.A. Verpoorte, S. Mehta, J.T. Edsall, Esterase activities of human carbonic anhydrase. J. Biol. Chem, 242 (1967) 4221
  13. H. Lineweaver, D. Burk, The determination of enzyme dissocation constants. J. Am. Chem. Soc, 57 (1934) 685
  14. M.M. Bradford, A rapid and sensi