Activatory effects of oxytetracycline on some α-carbonic anhydrases

Amaç & Hipotez:Üç alfa-karbonik anhidraz, insan karbonik anhidraz I, II (hCA I, II) ve koyun böbrek karbonik anhidraz (sCA) saflaştırıldı ve enzimlerle oksitetrasiklinin kinetik etkileşmeleri incelendi.

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Temel Bulgular & Veri:Saflaştırma prosedürü homojenat hazırlama ve Sepharose 4B-trizonsüllfanilamid afinite kramatografisinden oluştu.

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Sonuç & Katkı:Bu antibiyotik için % aktivite-[A] grafiği kullanılarak % aktivite değerleri belirlendi.

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Alan Parametreleri

Özet

Üç alfa-karbonik anhidraz, insan karbonik anhidraz I, II (hCA I, II) ve koyun böbrek karbonik anhidraz (sCA) saflaştırıldı ve enzimlerle oksitetrasiklinin kinetik etkileşmeleri incelendi. Saflaştırma prosedürü homojenat hazırlama ve Sepharose 4B-trizonsüllfanilamid afinite kramatografisinden oluştu. Oksitetrasiklin hCA I, II ve sCA enzim aktivitelerinde in vitro aktivatör etkisi gösterdi. Bu antibiyotik için % aktivite-[A] grafiği kullanılarak % aktivite değerleri belirlendi.

Abstract

Three μ-carbonic anhydrases, including human carbonic anhydrase I and II (hCA I, II) and sheep kidney carbonic anhydrase (sCA) were purified and kinetic interactions between the enzymes and oxytetracycline were investigated. The purification procedure was composed of preparation of homogenate (or hemolysate) and affinity chromatography on Sepharose 4B-tyrosine-sulfanilamide. Oxytetracycline exhibited in vitro activatory effects on hCA I, II and sCA enzymes activity. Activity % values for this antibiotic were determined by plotting activity % vs. [A].

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Bu Makaleye Atıf Yap

KÜFREVİOĞLU, Ö. İ., ŞENTÜRK, M., DEMİRDAĞ, R. (2022). Activatory effects of oxytetracycline on some α-carbonic anhydrases. Hacettepe Journal of Biology and Chemistry, Cilt: 39 - Sayı: 2, 207–212. https://ajindex.com/articles/529256

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