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Journals / Hacettepe Dişhekimliği Fakültesi Derg.(. Clinical Dentistry and Research) / 2005 / Cilt: 29 - Sayı: 4

Düşük şiddetli doğru akım ve düşük seviyede laser ışımasının periodontal ligament fibroblastları üzerine etkisinin incelenmesi: Ön çalışma

An assessment of the effects of low-intensity DC and low level laser irradiation on periodontal ligament fibroblasts: A preliminary study

Atilla ÖZDEMİR
Candan ÖZOĞUL
Yusuf TUNCA
Mehmet KÖSEM
Cüneyt GÖKSOY
A. Eralp AKCA
Serdar DEMİRTAŞ
Taner ÖZGÜRTAŞ
Pages
18–25
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However, 10 uA of DC electrical stimulation led to a decrease in the number of fibroblasts which was considered as a harmful effect on cells.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of this in vitro study was to compare the potential stimulatory effect of low-level laser irradiation and low-intensity direct current (DC) electrical stimulation on the proliferation of human periodontal ligament fibroblasts (PDLF).
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Key Findings & Data:Material and Methods: PDLF obtained from third molar periodontal ligaments were cultured under stan¬dard conditions and they were divided into 6 main groups (4 laser irradiation groups, control group and electrical stimulation group).
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Conclusion & Impact:However, 10 uA of DC electrical stimulation led to a decrease in the number of fibroblasts which was considered as a harmful effect on cells.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of this in vitro study was to compare the potential stimulatory effect of low-level laser irradiation and low-intensity direct current (DC) electrical stimulation on the proliferation of human periodontal ligament fibroblasts (PDLF). Material and Methods: PDLF obtained from third molar periodontal ligaments were cultured under stan¬dard conditions and they were divided into 6 main groups (4 laser irradiation groups, control group and electrical stimulation group). Each laser group were irradiated by 650 nm diode laser, operated at different power outputs. Specially designed culture flasks were used for electrical stimulation which was set up to lOuA. Each group was stimulated for four days. The collagen synthesis of fibroblasts in control, laser and electrical stimulation groups were evaluated by means of hydroxyproline level changes at the end of the 96th hour. Each group was also evaluated with TEM (Transmission Electron Microscope). Results: In the electrically stimulated group a slight increase was observed in hydroxyproline levels. Also collagen synthesis was observed in TEM analysis. In laser irradiated groups the basic result was the relevance between the laser output and the collagen synthesis. Conclusion: Our data indicated that the low level laser tout (5mW) increased proliferation rate of fibrob-f sts Additionally, TEM investigation revealed that the laser frequencies had no effect on the morphology of fibroblasts. On the other hand, DC electrical stimulation increased hydroxyproline level. However, 10 uA of DC electrical stimulation led to a decrease in the number of fibroblasts which was considered as a harmful effect on cells.

Özet

Amaç: Bu in vitro çalışmanın amacı, düşük güç lazer ışını ve DC elektrik akımının insan periodontal ligament fibrobroblastları (PDLF) üzerindeki potansiyel etkilerini incelemektir. Gereç ve Yöntem: PDLF insan üçüncü molar dişlerin periodontal ligamentlerinden elde edilen hücreler standart şartlar altında kültüre edilmiş ve 6 ana gruba ayrılmıştır (4 lazer grubu, kontrol grubu ve elektrik grubu). Her lazer grubu 650nm lik diod lazerin değişik akımları ile uyarılmıştır. Özel olarak tasarlanmış flaskta lOuA lik DC uygulanmıştır. Her grup dört gün süresince uyarılmışlardır. Gruplardaki kollajen sentezi hidroksiprolin seviyesinin 96. saat sonundaki değişikliği ile değerlendirilmiştir. Her grup ayrıca TEM incelemesine de tabi tutulmuştur. Bulgular: Elektrik ile uyarılmış grupta hidroksiprolin seviyesinde hafif bir artış izlenmiştir. TEM incelemesinde de kollajen sentezi görülmüştür. Lazer uygulanmış gruplarda elde edilen temel sonuç; lazer akımının gücü ile kollajen sentezi arasındaki ilişki olmuştur. Mitokondrial krista oluşumu ve kollajen sentezi lazer akımının gücü fazlalaştırıldığmda artmıştır.Sonuç: Verilerimiz 5mW lazer ışınının fibroblast proliferasyonunu arttırdığını göstermiştir. TEM incelemesi lazer akımlarının fibroblast morfolojisi üzerinde hiç bir etkisinin olmadığını belirtmiştir. On uA lik DC elektrik uygulaması ise kollajen sentezi ile ilgili olduğu düşünülen hidroksiprolin seviyesini arttırmıştır. Bununla beraber, 10xA lik DC uygulaması fibroblast sayısını azaltmıştır. Bu nedenle, hücreler üzerinde zararlı etkileri olabileceği düşünülmüştür.

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Cite This Article

ÖZDEMİR, A., ÖZOĞUL, C., TUNCA, Y., KÖSEM, M., GÖKSOY, C., AKCA, A. E., DEMİRTAŞ, S., ÖZGÜRTAŞ, T. (2022). Düşük şiddetli doğru akım ve düşük seviyede laser ışımasının periodontal ligament fibroblastları üzerine etkisinin incelenmesi: Ön çalışma. Hacettepe Dişhekimliği Fakültesi Derg.(. Clinical Dentistry and Research), Cilt: 29 - Sayı: 4, 18–25. https://ajindex.com/articles/528558

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References (10)

  1. Allendorf JDF, Bessler M, Huang J, kayton ML, Laird D, Nowygrod R, Treat MR. Helium-Neon laser irradiation at fluences of 1,2, and 4J/cm2 failed to accelerate wound healing as assessed by both wound contracture rate and tensile strength. Lasers Surg Med. 1997; 20(3): 340-345.
  2. Almeida-Lopes L, Rigau J, Amaro-Zangora R, Guidugli-Neto J, Jaeger MMM. Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence. Lasers Surg Med. 2001; 29(2): 179-184. Alverazom PM. The healing of superficial skin wound is stimulated by external electrical current. J Invest Derma. 1983; 81(2), 144-148.
  3. Atalay N, Aydın G, Işık B, Toktaş S. Accelerating wound healing with electrical current and the effect of current on tissue hydroxyproline content. J Biochem. 1992; 17: 15. Balboni GC, Zonefrati R, Brandi ML, Repice F. Effects of He-Ne/I.R. laser irradiation on two lines of normal human fibroblasts in vitro. Arch Italiono Anatom Embriol. 1986; 91: 179-188.
  4. Bednerska K, Rozga B, Kolodziejczyk K, Szosland D, LeykoW, Bryszewska M. Effect of low-power red light irradiation on the viability of human skin fibroblasts. Radiat Environl Biophys. 1998; 37: 215-217.
  5. Blumenthal NC, Ricci J, Breger L, Zychlinsky A, Solomon H, Chen GG et al. Effects of low-intensity AC and/or DC electromagnetic fields on cell attachment and induction of apoptosis. Bioelectromag. 1997; 18(3), 264-272.
  6. Bourguignon GJ, Bourguignon LYW. Electrical stimulation of human fibroblasts causes an increase in Ca++ influx and the exposure of additional insulin receptors. J Cell Physiol. 1989; 140(2): 379-385.
  7. Carillo JS, Calaayud J, manso FJ, Barberia E, Martinez JM, Donado M. A randomized double-blind clinical trial on the effectiveness of helium-neon laser in the prevention of pain, swelling and trismus after removal of impacted third molars. Int Dent J. 1990; 40: 31-36. Carranza FA. Glickman's clinical periodontology. 9th ed. In: The tooth supporting structures. Philadelphia: W.B.Saunders Co, 2002 pp. 36-57.
  8. Cheng K, Goldman RJ. Electric fields and proliferation in a dermal wound model: Cell cycle kinetics. Bioelectromag. 1998; 19: 68-74.
  9. Conlan MJ, Rapley JW, Cobb CM. Biostimulation of wound healing by low-energy laser irradiation. A review. J Clin Periodontol. 1996; 23: 492-496.
  10. Farndale RW, Murray JC. Pulsed electromagnetic fields promote collagen production in bone marrow fibroblasts via athermal mechanisms. Calcif Tissue Int. 1985;