Journals / Gazi Üniversitesi Mühendislik Mimarlık Fakültesi Dergisi / 2006 / Cilt: 21 - Sayı: 3

Examination of the effect of klinoptilolite and tween-20 on ethanol production by the measurement of biomass and metal ion

Klinoptilolit ve tween-20' nin etanol üretimi üzerine etkisinin biyokütle gelişimi ve metal iyonu değişimiyle incelenmesi

Pages
483–488
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Tween-20 showed no net effect on biomass yield and ethanol production.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this research in which Saccharomyces cerevisiae was used, the effect of addition of (1-10g/L) klinoptilolite KLN and Tween-20 (0,1- 10 g/L), on ethanol production was examined by the measurement of biomass concentration and metal ion concentration variations with respect to time during fermentation.
📊
Key Findings & Data:As a result, in the presence of 5 g/L KLN, production rate is increased from control culture value of 0.078 v/v.h to 0.104 v/v.h.
💡
Conclusion & Impact:Tween-20 showed no net effect on biomass yield and ethanol production.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this research in which Saccharomyces cerevisiae was used, the effect of addition of (1-10g/L) klinoptilolite KLN and Tween-20 (0,1- 10 g/L), on ethanol production was examined by the measurement of biomass concentration and metal ion concentration variations with respect to time during fermentation. The synthetic molasses was used. As a result, in the presence of 5 g/L KLN, production rate is increased from control culture value of 0.078 v/v.h to 0.104 v/v.h. From the ion analyses, it was observed that the KLN added to the medium decreased the concentration of Na+,Zn++ and Mn++ cations which are known to have toxic effect on glycolysis and cell viability. This effect occurred by means of the ion exchange and adsorption properties of KLN. Tween-20 showed no net effect on biomass yield and ethanol production.

Özet

Saccharomyces cerevisiae mayasının kullanıldığı bu çalışmada, fermentasyon ortamına değişen konsantrasyonlarda eklenen klinoptilolit (KLN) (1-10g/L) ve Tween-20’nin (0,1- 10 g/L), biyokütle gelişimi ve ortamdaki metal iyonu değişimi takip edilerek, etanol üretimi üzerindeki etkileri araştırılmıştır. Çalışmada yapay melas kullanılmıştır. Kontrol kültüre göre ortamda 5 g/L KLN olduğunda, kontrol kültürle 32 saatte ulaşılan etanol konsantrasyonuna 18 saatte ulaşılmış ve etanol üretim hızı 0,078 v/v.h değerinden 0,104 v/v.h değerine artış göstermiştir. KLN’nin ortamda bulunmasıyla, adsorpsiyon ve iyon değişim özelliği sayesinde, ortamda toksik miktarda bulunan Na+, Zn++ ve Mn++ iyonlarının miktarlarında azalma belirlenmiş ve bu durumun etanol hızını artıracak yönde etkilediği düşünülmüştür. Ortama Tween-20 eklenmesi durumunda biyokütle gelişimi ve etanol üretimi açısından olumlu yönde sonuç alınamamıştır.

🔖

Cite This Article

ERGUN, M., TOSUN, A. (2022). Examination of the effect of klinoptilolite and tween-20 on ethanol production by the measurement of biomass and metal ion. Gazi Üniversitesi Mühendislik Mimarlık Fakültesi Dergisi, Cilt: 21 - Sayı: 3, 483–488. https://ajindex.com/articles/524101

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Soares, E. V., Hebbelinck, K., Soares, H., “Toxic Effect Caused by Heavy Metals in the Yeast Saccharomyces cerevisiae: A Comparative Study”, Can. J. Microbiol./Rev. Can. Microbiol., Cilt 49 No 5, 336-343, 2003
  2. Fernandes, A. R., Correia, I., “The Activity of Plasma Membrane H+ATPase is Strongly Stimulated During Saccharomyces cerevisiae Adaptation to Growth under High Copper Stress” Yeast, Cilt 18, No 6, 511-521, 2001
  3. Kreder, G. C., “Yeast Assimilation of Trub-Bond Zinc”, Journal American Soc. Brew. Chem., Cilt 57, No 4, 129-132, 1999
  4. Ergun M., Mutlu F., Gürel Ö., “Effect of Addition of EDTA, Ferrocynide and Zeolite X on Ethanol Fermentation of Molasses”, J. Chem. Tech. Biotechnol., 68, 147-150, 1997
  5. Rosario, C., Raquel, A., Joaquium, M. S., Iborra, L., “Effect of Zeolite Addition on Ethanol Production from Glucose by Saccharomyces cerevisiae”, J. Chem. Technol. Biotechnol, 73, 377-384, 1998
  6. Tosun, A., Yapay Melastan Fermentasyon Yoluyla Etanol Üretimine Ortama Eklenecek Maddelerin Etkisi, Doktora Tezi, Gazi Üniversitesi, Fen Bilimleri Enstitüsü, 2005
  7. Zorpas, A. A., Constantinides, T., et. Al., “Heavy Metal Uptake by Natural Zeolite and Metals Partitioning in Sewage Sludge Compost”, Bioresource Tech., 72, 113-119, 2000
  8. Bauman, M., Mesaric, M., Ribar, S., Maric, V., Tudja, M., “Natural Zeolite Clinoptilolite Increases the Concentrations of Sphingoid Bases in The Yeast Yarrowia Lipolytica”, Journal of Basic Microbiology, Cilt 41, No 1, 7-16, 2001
  9. Lee, W. G., Lee, J. S., Lee, C. S., Shin, C. S., Kim, M. S., Park, S. C., Effect of Surfactants on Ethanol Fermentation Using Glucose and Cellulosic Hydrolyzates, Biotechnology Letters, Cilt 18, No 3, 299-304, 1996
  10. Wei, G., Li, Y., Du, G., Cheni J., “Effect of Surfactants on Extracellular Accumulation of Glutathione by Saccharomyces cerevisiae ”, Process Biochemistry, Cilt 38, No 8, 1133-1138, 2002
  11. Oderinde, R. A., Ngoka, L. C., Adesogan, “Comparative Study of the Effect of Ferrocyanide and EDTA on the Production of Ethyl Alcohol from Molasses by Saccharomyces cerevisiae”, Biotechnology and Bioengineering, 28, 1462- 1465, 1986
  12. Pandey, K., Agarwal P. K., “Effect of EDTA, Potassium Ferrocyanide and Sodium Potassium Tartarate on the Production of Ethanol from Molasses by Saccharomyces cerevisiae”, Enzyme Microb. Technol, 15, 887-891, 1993
  13. Alfenore, S., Moline, C., Guillouet, J. L., Gome, G., Benbadis, L., “Improving Ethanol Production and Viability of Saccharomyces