SavedAdd your journal

Journals / Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi / 2018 / Cilt: 32 - Sayı: 2

Effects of Some Antioxidant Additives on Spermatological Parameters, Oxidative Stress and DNA Damage After Freezing-Thawing Process in Ram Semen

Koç Spermasına Katılan Bazı Antioksidanların Dondurma ve Çözdürme Sonrası Spermatolojik Parametreler, Oksidatif Stres ve DNA Hasarı Üzerine Etkileri

Fatih AVDATEK
Mustafa GÜNDOĞAN
Pages
135–142
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The increases inhypoosmotic eosin staining test (H+/E- type) spermatozoa were significant (P

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, determing the effects of antioxidants on the computer assisted sperm analysis(CASA), oxidative stress parameters and the DNA damage of the thawed sperm was aimed.Semen collected from 10 Pirlak rams was divided into five equal aliquots and diluted with the trisbase extender containing taurine, trehalose, trolox, lipoic acid and no antioxidant (control), cooledto 5 ⁰ C and frozen in 0,25 mL French straws in liquid nitrogen vapour and stored in liquid nitrogen.In the thawed semen, the highest values were detected in taurine group with regard to subjectivemotility and CASA motility, average path velocity μm/s (VAP) and straight linear velocity μm/s(VSL).
📊
Key Findings & Data:The lowest values in spermatozoa with abnormal head, middle piece and acrosome werefound in all antioxidant groups when compared with the control group.
💡
Conclusion & Impact:The increases inhypoosmotic eosin staining test (H+/E- type) spermatozoa were significant (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, determing the effects of antioxidants on the computer assisted sperm analysis(CASA), oxidative stress parameters and the DNA damage of the thawed sperm was aimed.Semen collected from 10 Pirlak rams was divided into five equal aliquots and diluted with the trisbase extender containing taurine, trehalose, trolox, lipoic acid and no antioxidant (control), cooledto 5 ⁰ C and frozen in 0,25 mL French straws in liquid nitrogen vapour and stored in liquid nitrogen.In the thawed semen, the highest values were detected in taurine group with regard to subjectivemotility and CASA motility, average path velocity μm/s (VAP) and straight linear velocity μm/s(VSL). The lowest values in spermatozoa with abnormal head, middle piece and acrosome werefound in all antioxidant groups when compared with the control group. The increases inhypoosmotic eosin staining test (H+/E- type) spermatozoa were significant (P

Özet

Bu çalışmada, spermaya katılan antioksidanların dondurma çözdürme sonrası spermatolojik, bigisayar destekli sperma analiz cihazı (CASA), oksidatif stres parametreleri ve DNA hasarları üzerine etkilerinin belirlenmesi amaçlandı. Afyonkarahisar koşullarında yetiştirilen 2-3 yaşlı Pırlak ırkına ait toplam 10 koçtan alınan spermalar birleştirilip 5 eşit hacme bölünerek kontrol başta olmak üzere taurin, trehaloz, trolox ve lipoik asit içeren Tris bazlı sulandırıcılar ile sulandırıldı. Sulandırılan örnekler 0.25 mL’lik payetlerde 5 0C’de 3 saat ekilibrasyona tabi tutulduktan sonra sıvı azot buharında dondurularak sıvı azot içerisinde (-196 ⁰ C) saklandı. Çözdürülen spermalarda subjektif motilite, CASA motilitesi, ortalama rota hızı μm/s (VAP) ve düz çizgi hızı μm/s (VSL) açısından taurin grubunda en yüksek değerler tespit edildi. Anormal spermatozoon baş, orta kısım ve akrozom oranı açısından kontrol grubuna göre tüm gruplarda en düşük değerler elde edildi. Spermatozoon hipo-ozmotik eosin boyama testi (HE-test) parametrelerinden H+/E- tip spermatozoonlarda kontrol grubuna göre taurin, trehaloz ve lipoik asit gruplarındaki artışlar önemli (P

🔖

Cite This Article

AVDATEK, F., GÜNDOĞAN, M. (2022). Effects of Some Antioxidant Additives on Spermatological Parameters, Oxidative Stress and DNA Damage After Freezing-Thawing Process in Ram Semen. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, Cilt: 32 - Sayı: 2, 135–142. https://ajindex.com/articles/514946

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Bailey JL, Bilodeau JF. Cormeir semen cryopereservation in domestic animals: A damaging and capacitating phenomenon. J. of And 2000; 21: 1-7.
  2. Oehninger S, Duru NK, Srisombut C, Morshedi M. Assessment of sperm cryodamage and strategies to ımprove outcome. Mol. Cell. Endocrinol 2000; 169: 3-10.
  3. Agarwal A, Prabakaran S, Allamaneni S. What an andrologist/urologist should know about free radicals and why. Urology 2006; 67: 2-8.
  4. Perıs SI, Bilodeau JF, Dufour M, Bailey JL. Impact of cryopreservation and reactive oxygen species on DNA integrity, lipit peroxidation, and functional parameters in ram sperm. Mol Reprod Dev 2007; 74: 878-892.
  5. Aitken J, Clarkson JS, Fishel S. Generation of reactive oxygen species, lipit peroxidation, and human sperm function. Biol. Reprod 1989; 41: 183-197.
  6. Chen Y, Foote RH, Brockett CC. Effect of sucrose, trehalose, hypotaurine, taurine and blood serum on survival of frozen bull sperm. Crybiol 1993; 30: 423-431.
  7. Hua Li J, Qin Ling Y, Jing Fan J, Ping Zhang X, Cui S. Expression of cysteine sulfinate decarboxylase (CSD) in male reproductive organs of mice. Histochem Cell Biol 2006; 125: 607-613.
  8. Aisen EG, Quıntana M, Medina V, Morello H, Venturino A. Ultramicroscopic and biochemical changes in ram spermatozoa cryopreserved with trehalose-based hypertonic extenders. Cryobiol 2005; 50: 239-249. Akiyama M. In vivo scavenging effects of ethylcysteine on reactive oxygen species in human semen. Nippon Hinyokika Gakkai Zasshi 1999; 90: 421-428.
  9. Rice D, Kennedy S. Vitamin E: Function and effects of deficiency. Br Vet J 1988; 144: 482-492.
  10. Roy S, Packer L. Redox regulation of cell functions by alpha-lipoate: Biochemical and molecular aspects. Biofactors 1998; 8: 17-21.
  11. Schafer S, Holzmann A. The use of transmigration and Spermac TM stain to evaluate epididymal cat spermatozoa. Anim Reprod Sci 2000; 59: 201-211.
  12. Gündoğan M, Yeni D, Avdatek F, Fidan AF. Influence of sperm concentration on the motility, morphology, membrane and DNA integrity along with oxidative stress parameters of ram sperm during liquid storage. Anim Reprod Sci 2010; 122: 200-207.
  13. Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1990; 186: 421-430.
  14. Sedlak J, Lindsay RHC. Estimation of total, protein bound and nonprotein sulfhydryl groups in tissue with Ellmann’s reagent. Anal Biochem 1968: 25; 192-205.
  15. Geva E, Lessing JB, Lerner-Geva L, Amit A. Free radicals, antioxidants and human spermatozoa. Clinical Implications 1998; 13: 1422