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Journals / Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi / 2009 / Cilt: 23 - Sayı: 2

Prevalence of Arcobacter species in ground meat from cattle and sheep using multiplex PCR techniques

Sığır ve koyun kıymalarında Arcobacter türlerinin bulunma sıklıklarının multipleks PCR tekniği ile belirlenmesi

Nurhan ERTAŞ
Yusuf DOĞRUER
Pages
95–100
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, marketed ground meat samples in Kayseri province were contaminated with Arcobacter, particularly very often with A. butzleri but less often with A. skirrowi.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, presence of Arcobacter species in ground meat from cattle and sheep in Kayseri province (Turkey) were investigated.
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Key Findings & Data:A total of 200 samples (100 cattle ground meat and 100 sheep ground meat) were examined.
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Conclusion & Impact:In conclusion, marketed ground meat samples in Kayseri province were contaminated with Arcobacter, particularly very often with A. butzleri but less often with A. skirrowi.
🔬Design:Research Article
📍Scope:200 samples
⚙️Variables:Domain Parameters

Abstract

In this study, presence of Arcobacter species in ground meat from cattle and sheep in Kayseri province (Turkey) were investigated. Identification of Arcobacter species in isolates of ground meat from cattle and sheep using Multiplex PCR (mPCR) techniques were carried out. A total of 200 samples (100 cattle ground meat and 100 sheep ground meat) were examined. The samples were homogenized with distilled water and incubated in Arcobacter Enrichment Media (AZB) at 30 oC for 48 hours. After the incubation in AZB, grown suspected cultures were inoculated into agar using membrane Filtration Techniques. Eighty-five out of 200 meat samples were isolated Arcobacter spp. positive. Of these positive samples, 79 (92.9%) A. butzleri and 6 (7.1%) A. skirrow were detected. Forty out of 100 cattle meat ground were identified with A. butzleri (40%) and only 2 (2%) with A. skirrow, whereas 39 (39%) with A. butzleri and 4 (4%) with A. skirrow in ground sheep meat samples. In conclusion, marketed ground meat samples in Kayseri province were contaminated with Arcobacter, particularly very often with A. butzleri but less often with A. skirrowi. It was also concluded that Arcobacter species particularly A. butzleri may have a risk factor for human health with the fact that A. butzleri causes enteritis, chronic diarrhea, and septicemia in humans.

Özet

Bu çalışmada, Kayseri ilindeki farklı satış noktalarından alınan sığır ve koyun kıyması örneklerinde Arcobacter türlerinin varlığı araştırıldı. Klasik kültür yöntemleri ile izole edilen kültürler multiplex Polimerase Chain Reaction (m PCR) tekniği ile tür düzeyinde identifiye edildi. İncelenen 200 kıyma numunesinin 85’inde Arcobacter spp. izole edildi. İzolatlara uygulanan mPCR işlemi sonucunda 85 pozitif örneğin 79’u (% 92,9) A. butzleri, 6’sı (% 7,1) A.skirrowi olarak tespit edildi. 100 adet sığır kıyma örneğinin 40’ı (% 40) A. butzleri, 2’si (% 2) A. skirrowi olarak identifiye edildi. Koyun kıyma örneğinin 39’u (% 39) A. butzleri olarak, 4’ü (% 4) A. skirrowi olarak identifiye edildi. Sonuç olarak Kayseri ilinde satışa sunulan kıymaların Arcobacter türlerinden başta A. butzleri olmak üzere A. skirrowi ile kontamine olduğu belirlenmiştir. Arcobacter türlerinin, özellikle A. butzleri’nin insanlarda enteritis, kronik diyare ve septisemi vakalarından izole edilmesi nedeni ile kıymalardaki A. butzleri oranı halk sağlığı açısından önemli bir risk faktörü olabileceği belirlendi.

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Cite This Article

ERTAŞ, N., DOĞRUER, Y. (2022). Prevalence of Arcobacter species in ground meat from cattle and sheep using multiplex PCR techniques. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, Cilt: 23 - Sayı: 2, 95–100. https://ajindex.com/articles/515267

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References (13)

  1. Atabay HI, Aydin F, Helps C, Şahin M. Identification of Arcobacter isolates using 16S rDNA restriction endonuclease analysis of PCR products (ARDRA), Vet Hek Mikrobiol Derg 2001; 1(1): 70-77
  2. Houf K, de Zutter L, Hoof JV, Vandamme P. Occurrence and distribution of Arcobacter species in poultry processing, J Food Protect 2002; 65(8): 1233-1239
  3. Houf K, de Zutter L, Hoof JV, Vandamme P. Assesment of the genetic diversity among Arcobacter isolated from poultry products by using two PCR- Based typing methods, Appl Environ Microbiol 2002; 68(5): 2172-2178
  4. Rivas L, Fegan N, Vanderline P Isolation and characterisation of Arcobacter butzleri from meat, Int J Food Microbiol 2004; 91: 31-44
  5. Vandenberg O, Dediste A, Houf K, Ibekwem S, Souayah H, Cadranel S, Douat N, Zissis G, Butzler JP, and Vandamme P. Arcobacter species in human. Emerg Infect Dis 2004; 10(10), 1863-1867
  6. Van Driessche E, Houf K, Vangroenweghe F, Nollet N, De Zutter L, Hoof JV. Prevalence, enumeration and strain variation of Arcobacter species in the faeces of healthy cattle in Belgium, Vet Microbiol 2005; 105:149-154
  7. Wesley IV, Schroeder-Tucker L, Baetz AL, Dewhirst FE and Paster BJ. Arcobacter-specific and Arcobacter butzleri-specific 16S rRNA-based DNA probes, J Clin Microbiol 1995; 33(7): 1691-1698
  8. Antolin A, Gonzalez I, Garcia T, Hernandez PE, Martin R. Arcobacter spp. enumeration in poultry meat using a combined PCR-ELISA assay, Meat Sci 2001; 59: 169- 174
  9. Houf K, Tutenel A, de Zutter L, Hoof JV, Vandamme P. Devolopment of a multiplex PCR assay for smultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii, FEMS Microbiol Lett 2000; 193: 89-94
  10. Houf K, Devriese LA, de Zutter L, Hoof JV. Vandamme P. Devolopment of a new protocol for the isolation and quantification of Arcobacter species from poultry products, Int J Food Microbiol 2001; 71: 189-196
  11. Houf K, Devriese LA, de Zutter L, Hoof JV, Vandamme P. Susceptibility of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii to antimicrobial agents used in selective media, J Clin Microbiol 2001; 39 (4): 1654-1656
  12. Kabeya H, Maruyama S, Morita Y, Kubo M, Yamamoto K, Arai S, Izumi T, Kobayashi Y, Abe M, Katsube Y, Mikami T Distribution of Arcobacter species among livestock in Japan, Vet Microbiol 2003; 93: 153-158
  13. Kabeya H, Kobayashi Y, Maruyama S, Mikami T (2003b) One-step polymerase chain reaction-based typing of Arcobacter sp