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Journals / Fırat Üniversitesi Sağlık Bilimleri Dergisi / 2008 / Cilt: 22 - Sayı: 3

Effects of some nitrosamines on pyruvate kinase activity in tissues of rats

Bazı nitrozaminlerin ratlarda doku pirüvat kinaz aktivitesi üzerine etkileri

Fatih Mehmet KANDEMİR
İzzet KARAHAN
Seval YILMAZ
Pages
163–168
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

While administration of N-NDEA in liver and kidney tissues wasn’t significantly change at enzyme activity, it was found significantly decrease in pyruvate kinase activities of liver (p

📌 3 Key Takeaways

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Objective & Hypothesis:In study, we investigated effects on pyruvate kinase activity following prolonged low amounts administrations in drinking water of some nitrosamines in rats.
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Key Findings & Data:Rats were divided into four groups and each group had 12 animals.
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Conclusion & Impact:While administration of N-NDEA in liver and kidney tissues wasn’t significantly change at enzyme activity, it was found significantly decrease in pyruvate kinase activities of liver (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In study, we investigated effects on pyruvate kinase activity following prolonged low amounts administrations in drinking water of some nitrosamines in rats. Rats were divided into four groups and each group had 12 animals. Group I was control group. Groups 2, 3 and 4 administered N-Nitrosodiethylamine (N-NDEA), 1-Nitrosopiperidine (1-NPip) and N-Nitrosopyrolidine (N-NPyr) at the amount of 200 ppb with drinking water for 30 successive days, respectively. Pyruvate kinase activities in blood, liver and kidney tissue samples were measured according to the method of Beutler following the administrations. No changes in blood pyruvate kinase activity were observed during prolonged administrations of N-NDEA, 1-NPip and N-NPyr in rats according to the control group. While administration of N-NDEA in liver and kidney tissues wasn’t significantly change at enzyme activity, it was found significantly decrease in pyruvate kinase activities of liver (p

Özet

Çalışmada; ratlara düşük dozda, uzun süreli olarak bazı nitrozaminlerin içme suyu ile verilmesinin pirüvat kinaz aktivitesi üzerine etkileri araştırıldı. Ratlar her grupta 12 hayvan olacak şekilde 4 gruba bölünerek, birinci grup kontrol olarak ayrıldı. Grup 2, 3 ve 4’teki ratlara sırasıyla N-Nitrozodietilamin (N-NDEA), 1-Nitrozopiperidin (1-NPip) ve NNitrozopirolidin (N-NPir) 30 gün süreyle 200 ppb miktarında içme suyuna katılarak verildi. Uygulamaları takiben alınan kan ile karaciğer ve böbrek doku örneklerinde Beutler yöntemine göre pirüvat kinaz aktivitesi ölçüldü. Uzun süreli N-NDEA, 1-NPip ve N-NPir uygulamaları kan pirüvat kinaz aktivitesinde kontrol grubuna göre değişiklik oluşturmadı. Karaciğer ve böbrek dokularında NNDEA verilmesi enzim aktivitesini önemli derecede değiştirmezken; 1-NPip ve N-NPir uygulamaları sonucunda kontrol grubuna göre karaciğer ve böbrek doku pirüvat kinaz aktivitelerinin azaldığı saptandı (p

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Cite This Article

KANDEMİR, F. M., KARAHAN, İ., YILMAZ, S. (2022). Effects of some nitrosamines on pyruvate kinase activity in tissues of rats. Fırat Üniversitesi Sağlık Bilimleri Dergisi, Cilt: 22 - Sayı: 3, 163–168. https://ajindex.com/articles/514114

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