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Journals / Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi / 2021 / Cilt: 35 - Sayı: 2

Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease

Beyaz Kas Hastalıklı Kuzularda 4-Hidroksi-2-Nonenal, Ditirozin and 8-Hidroksi-2- Deoksiguanozin Ekspresyonlarının Değerlendirilmesi

Emin KARAKURT
Serpil DAĞ
Ayfer YILDIZ
Hilmi NUHOĞLU
Enver BEYTUT
Özhan KARATAŞ
Ali Sefa MENDİL
Pages
109–113
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

A significant difference (P

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to investigate Reactive Oxygen Species (ROS)-induced lipidperoxidation, protein and DNA damage with oxidative stress markers such as 4-hydroxy-2- nonenal (4-HNE), dityrosine (DT) and 8-hydroxy-2-deoxyguanosine (8-OHdG) in lambs withWhite Muscle Disease (WMD).
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Key Findings & Data:The material of this study consisted of tissue samples taken from20 sheep with WMD and 6 healthy control groups brought to Pathology Department for routinehistopathological diagnosis between 2012 and 2019.
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Conclusion & Impact:A significant difference (P
🔬Design:Research Article
📍Scope:2012 Verileri
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to investigate Reactive Oxygen Species (ROS)-induced lipidperoxidation, protein and DNA damage with oxidative stress markers such as 4-hydroxy-2- nonenal (4-HNE), dityrosine (DT) and 8-hydroxy-2-deoxyguanosine (8-OHdG) in lambs withWhite Muscle Disease (WMD). The material of this study consisted of tissue samples taken from20 sheep with WMD and 6 healthy control groups brought to Pathology Department for routinehistopathological diagnosis between 2012 and 2019. In macroscopic examinations, chalky-whitelarge necrotic areas on the epicardial and especially on the endocardial surfaces of the right andleft ventricular walls were observed. In microscopic examinations, it was observed that thedegenerated muscle fibers in the heart were homogeneously pink in color, lost their cytoplasmiccitration, swelled and their nuclei were pyknotic. Calcifications in necrotic and degenerated areaswere detected. All white muscle disease cases were immune positive for 4-HNE, DT and 8-OHdGexpressions in heart tissues. A significant difference (P

Özet

Bu çalışmada, beyaz kas hastalıklı kuzularda 4-hidroksi-2-nonenal (4-HNE), ditirozin (DT) ve 8- hidroksi-2-deoksiguanozin (8-OHdG) gibi oksidatif stres belirteçleri ile Reaktif Oksijen Türleri(ROT) kaynaklı lipid peroksidasyonu, protein ve DNA hasarının araştırılması amaçlanmıştır. Buçalışmanın materyalini 2012-2019 yılları arasında rutin histopatolojik tanı için Patoloji AnabilimDalı’na getirilen beyaz kas hastalıklı 20 koyundan alınan doku örnekleri ve 6 sağlıklı kontrol grubuoluşturdu. 4-HNE, DT ve 8-OHdG ekspresyonları immünohistokimyasal ve immünofloresanyöntemlerle değerlendirildi. Makroskopik incelemelerde epikardiyal ve özellikle endokardiyalyüzeylerde, sağ ve sol ventrikül duvarlarında tebeşirimsi beyaz büyük nekrotik alanlar gözlemlendi.Mikroskobik incelemelerde kalpteki dejenere olmuş kas liflerinin homojen pembe renkte olduğunu,sitoplazmik sitrifikasyonunu kaybettiğini, şiştiği ve çekirdeklerinin piknotik olduğu gözlemlendi.Nekrotik ve dejenere alanlarda kalsifikasyonlar tespit edildi. Tüm beyaz kas hastalığı vakaları kalpdokularında 4-HNE, DT ve 8-OHdG ekspresyonu yönünden immün pozitifti. İmmünohistokimyasalve immünofloresan boyama sonuçlarında 4-HNE, DT ve 8-OHdG'ye karşı immünreaktiviteaçısından gruplar arasında anlamlı farklılık (P

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Cite This Article

KARAKURT, E., DAĞ, S., YILDIZ, A., NUHOĞLU, H., BEYTUT, E., KARATAŞ, Ö., MENDİL, A. S. (2022). Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, Cilt: 35 - Sayı: 2, 109–113. https://ajindex.com/articles/507668

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