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Journals / Epilepsi / 2016 / Cilt: 22 - Sayı: 3

Effects of Environmental Enrichment on Hippocampal Electrophysiological Changes in the Pentylenetetrazole Model of Epilepsy

Pentilentetrazol İle Oluşturulmuş Epilepside Zenginleştirilmiş Çevrenin Hipokampal Elektrofizyolojik Değişikliklere Etkisi

Seda ARTIŞ
Cem SÜER
Seval KELOĞLAN
Soner BİTİKTAŞ
Nazan DOLU
Journal
Epilepsi
Pages
93–101
Publication Date
2022-10-17
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However, according to electrophysiological recordings, environmental enrichment did not provide positive effect on LTP in epileptic rats.Conclusion: Enriched environment with ongoing PTZ-induced kindling procedure may lead to exaggeration of seizures due to stress as resultof corruption of safe, familiar environment.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: Epilepsy is a neurological disorder characterized by functional/morphological changes in the hippocampus.
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Key Findings & Data:These functionalchanges arise as increase or decrease in synaptic plasticity in experimental animals.
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Conclusion & Impact:However, according to electrophysiological recordings, environmental enrichment did not provide positive effect on LTP in epileptic rats.Conclusion: Enriched environment with ongoing PTZ-induced kindling procedure may lead to exaggeration of seizures due to stress as resultof corruption of safe, familiar environment.
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: Epilepsy is a neurological disorder characterized by functional/morphological changes in the hippocampus. These functionalchanges arise as increase or decrease in synaptic plasticity in experimental animals. The present study was an investigation of the effect ofenriched environment on hippocampal functional changes in epileptic rats.Methods: The pentylenetetrazole (PTZ) kindling model was used on young male Wistar rats. Rats in the epileptic and control groups werereared for 1 month in standard cage or enriched cage (EC). Subsequently, all animals were given Morris water maze (MWM) test and in vivorecording of long-term potentiation (LTP) in medial perforant pathway-dentate gyrus synapses in hippocampus was made.Results: Statistically significant earlier kindling epileptogenesis in rats housed in EC was observed. Epileptic rats had poor performance inMWM, but enriched environment improved their performance. However, according to electrophysiological recordings, environmental enrichment did not provide positive effect on LTP in epileptic rats.Conclusion: Enriched environment with ongoing PTZ-induced kindling procedure may lead to exaggeration of seizures due to stress as resultof corruption of safe, familiar environment.

Özet

Amaç: Epilepsi, hipokampusta işlevsel/morfolojik değişikliklerle karakterize nörolojik bir hastalıktır. Deney hayvanlarında bu işlevsel değişiklikler sinaptik plastisitede artma ya da azalma şeklinde ortaya çıkmaktadır. Sunulan çalışmada amacımız, zenginleştirilmiş çevrenin hipokampal işlevsel değişiklikler üzerine olan etkisini epileptik sıçanlarda araştırmaktı. Gereç ve Yöntem: Pentilentetrazol (PTZ) ile oluşturulan kindling modeli sıçanlara uygulandı. Sıçanlar epileptik ve kontrol grubu olarak standart kafes ya da zenginleştirilmiş kafeste bir ay kaldılar. Ardından bütün hayvanlara Morris yüzme testi yapıldı ve hipokampusa ait medial perforan yol dentat girus sinapslarından in vivo olarak uzun süreli güçlenme (USG) kaydı alındı. Bulgular: Çalışmamızda zenginleştirimiş kafeste bulunan epileptik sıçanların daha erken tutuştuğu görüldü. Epileptik sıçanlar morris yüzme testinde zayıf performans gösterdiler ve zenginleştirilmiş çevre bu durumu iyileştirdi. Bununla birlikte elektrofizyolojik kayıt bulgularımızda, zenginleştirilmiş çevre epileptik sıçanlarda USG üzerinde pozitif etki göstermedi. Sonuç: Sonuç olarak, PTZ-nedenli kindling modeli ile birlikte zenginleştirilmiş çevre uygulaması, sıçanlarda alışkın olunan güvenli çevrenin bozulması nedeniyle ortaya çıkan strese bağlı olarak nöbetleri şiddetlendirebilir

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Cite This Article

ARTIŞ, S., SÜER, C., KELOĞLAN, S., BİTİKTAŞ, S., DOLU, N. (2022). Effects of Environmental Enrichment on Hippocampal Electrophysiological Changes in the Pentylenetetrazole Model of Epilepsy. Epilepsi, Cilt: 22 - Sayı: 3, 93–101. https://ajindex.com/articles/501680

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