Journals / Düzce Üniversitesi Bilim ve Teknoloji Dergisi / 2018 / Cilt: 6 - Sayı: 1

Viable But Non Culturable State as a Survival Strategy in Bacteria and The Important of This State

Bakterilerde Yaşam Stratejisi Olarak Canlı Fakat Kültürü Yapılamayan Durum ve Önemi

Pages
108–122
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The genetic mechanism of VBNC is not yet known.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, dormancy state which is a life strategy be able to maintain their lives under stress conditions of the bacteria which are not differentiating feature which can not form a sport or cyst) can be sustained under the stress conditions was investigated.
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Key Findings & Data:The Viable But Non Culturable State (VBNC) hypothesis is metabolically active but not cultured in the bacterial cells by known laboratory methods.
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Conclusion & Impact:The genetic mechanism of VBNC is not yet known.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Dormansi · Aktif fakat kültürü yapılamayan(VBNC) · Stres

Abstract

In this study, dormancy state which is a life strategy be able to maintain their lives under stress conditions of the bacteria which are not differentiating feature which can not form a sport or cyst) can be sustained under the stress conditions was investigated. The Viable But Non Culturable State (VBNC) hypothesis is metabolically active but not cultured in the bacterial cells by known laboratory methods. Due to this situation, there is a great problems in isolating and counting bacteria from natural environments. Furthermore, a conventional method that can be applied to both all bacteria and under all conditions has not yet been developed. This situation, which is very important for human health, needs to be solved. The genetic mechanism of VBNC is not yet known.

Özet

Bu çalışmada farklılaşma özelliği olmayan (spor yada kist oluşturamayan) bakterilerin stres koşulları altında yaşamlarını devam ettirebilmek amacı ile oluşturdukları bir yaşam stratejisi olan dormansi durumu incelenmiştir. Canlı Fakat Kültürü Yapılamayan Durum (VBNC) hipotezi metabolik olarak aktif fakat bilinen laboratuar metodları ile kültüre edilemeyen bakteriyal hücrelerin girmiş olduğu bir bölünememe durumunu tanımlamaktadır. Bu durum nedeni ile doğal ortamlardan bakterilerin izolasyonları ve sayımlarının yapılmasında büyük problemler vardır. Ayrıca henüz bütün bakterilere ve bütün şartlarda uygulanabilecek geleneksel bir metod geliştirilememiştir. İnsan sağlığı açısından oldukça önemli olan bu durumun çözülmesi gerekmektedir. Fakat VBNC’nin henüz genetik mekanizması bilinmemektedir.

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Cite This Article

DARCAN, C. (2022). Viable But Non Culturable State as a Survival Strategy in Bacteria and The Important of This State. Düzce Üniversitesi Bilim ve Teknoloji Dergisi, Cilt: 6 - Sayı: 1, 108–122. https://ajindex.com/articles/494454

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References (10)

  1. D. McDougald, S. A. Rice, D. Weichart and S. Kjelleberg, “Nonculturability: adaptation or debilitation?,” FEMS Microbiology Ecology, vol. 25, pp. 1-9, 1998
  2. G. Bogosian, P. J. Morris and J. P. O’neil, “A mixed culture recovery method indicates that enteric bacteria do not enter the viable but nonculturable state,” Applied Environmental Microbiology, vol. 64, no. 5, pp. 1736-1742, 1998
  3. F. Joux, P. Lebaron and M. Trousellier, “Succession of cellular states in a Salmonella Typhimurium population during starvation in artificial seawater microcosms,” FEMS Microbiology Ecology, vol. 22, pp. 65-76, 1997
  4. H. S. Xu, N. Roberts, F. L. Singleton, R. W. Attwell, D. J. Grimes and R. R. Colwell, “Survival and viability of noncultrable Escherichia coli and Vibrio cholerae in the estuarine and marine environment,” Microbial Ecology, vol. 8, pp. 313-323, 1982
  5. S. Kjelleberg, M. Hermansson and P. Marden and G. W. Jones “The transient phase between growth and nongrowth of heterotrophic bacteria, with emphasis on the marine environment.” Anneal Review Microbiology, vol. 41, pp. 25-49, 1987
  6. K. Linder and J. D. Oliver, “Membrane fatty acid and virulence changes in the viable but nonculturable state of Vibrio vulnificus,” Applied Environmental Microbiology, vol. 55, no. 11, pp. 2837-2842, 1989
  7. R. R. Colwell, I. T. Knight, C. Somerville, S. Shults and C. W. Kaspar, “Viable but non-culturable phenomenon in relationship to starvation/survival, ‘’Injury’’, and strategies for survival of bacteria in the environment.” Recent Advances ın Microbial Ecology, 5th International Symposium on Microbial Ecology, Japan, pp. 85-88, 1989 [8] Y. Tanaka, N. Yamaguchi and M. Nasu, “Viability of Escherichia coli O157:H7 in natural river water determined by the use of flow cytometry,” Journal Applied Microbiology, vol. 88, pp. 228-238, 2000
  8. A. Ultee, N. Souvatzi, K. Maniadi and H. König, “Identification of the culturable and nonculturable bacterial population in ground water of a municipal water supply in Germany,” Journal Applied Microbiology, vol. 96, pp. 560-568, 2004
  9. A. A. Gavriel, J. P. Landre and A. J. Lamb, “Incidence of mesophilic Aeromonas within a public drinking water supply in north-east Scotland,” Journal Applied Microbiology, vol. 84, pp. 383-392, 1998
  10. D. Weichart, D. McDougald, D. Jacobs and S. Kjelleberg, “In situ analysis of nucleic acids in cold-induced nonculturable Vibrio vulnificus,” Applied Environmental Microbiology, vol. 63, no.