Journals / Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi / 2026 / Cilt: 5 Sayı: 1

Yeni Nesil Dizileme Teknolojisine Genel Bakış

Osman Türkyılmaz(Bilecik Şeyh Edebali Üniversitesi, Fen Bilimleri Enstitüsü, Moleküler Biyoloji ve Genetik Anabilim Dalı)
Pages
41–49
Publication Date
2018-06-28
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Bu derlemede genel olarak dizileme teknolojilerinden ve yeni nesil dizileme teknolojisinin kullanıldığı omik alanlardan bahsedilmektedir.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:DNA, RNA ve metilasyon dizilemesi için kullanılan yeni nesil dizileme teknolojileri bir çok bilim dalı üzerinde büyük etkiler oluşturarak bilimsel çalışmalara yeni bakış açıları kazandırmıştır.
📊
Key Findings & Data:Yeni nesil dizileme büyük ölçekli dizileme kapasitesi ile Sanger dizilemesine kıyasla çok daha düşük maliyetlere sahiptir.
💡
Conclusion & Impact:Bu derlemede genel olarak dizileme teknolojilerinden ve yeni nesil dizileme teknolojisinin kullanıldığı omik alanlardan bahsedilmektedir.
🔬Design:Survey & Empirical Study
📍Scope:Comprehensive Sample
⚙️Variables:Yeni Nesil Dizileme · Yüksek Çıktılı Dizileme · Omik

Özet

DNA, RNA ve metilasyon dizilemesi için kullanılan yeni nesil dizileme teknolojileri bir çok bilim dalı üzerinde büyük etkiler oluşturarak bilimsel çalışmalara yeni bakış açıları kazandırmıştır. Yeni nesil dizileme büyük ölçekli dizileme kapasitesi ile Sanger dizilemesine kıyasla çok daha düşük maliyetlere sahiptir. Yeni nesil dizilemenin maliyet ve hız avantajları daha önceden neredeyse imkansız olarak düşünülen çalışmaların yapılabilir hale gelmesine olanak sağlamıştır. Bu gelişmeler yapısal ve işlevsel genomik anlayışımızı, temel genomikten entegre sistomiğe kadar değişen bir çok omik kavramı ile genişletmiştir. Bu derlemede genel olarak dizileme teknolojilerinden ve yeni nesil dizileme teknolojisinin kullanıldığı omik alanlardan bahsedilmektedir.

🔖

Cite This Article

Darcan, C., Türkyılmaz, O. (2018). Yeni Nesil Dizileme Teknolojisine Genel Bakış. Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi, Cilt: 5 Sayı: 1, 41–49. https://ajindex.com/articles/1264716

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References (82)

  1. Heather, J.M., Chain, B. “The sequence of sequencers: The history of sequencing DNA”, Genomics, vol. 107, pp. 1–8, 2016
  2. Fiers, W., Contreras, R., Duerinck, F., Haegeman, G., Iserentant, D., et al., “Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene”, Nature, vol. 260, pp. 500–507, 1976
  3. Maxam, A.M., Gilbert, W., “A new method for sequencing DNA”, Proc Natl Acad Sci USA, vol. 74, pp. 560 – 564, 1977
  4. Sanger, F., Nicklen, S., Coulson, A.R., “DNA sequencing with chain-terminating inhibitors”, Proc Natl Acad Sci, vol. 74, pp. 5463−5467, 1977
  5. Mardis, E.R., “The impact of next-generation sequencing technology on genetics”, Trends in Genetics, vol. 24, pp. 133-141, 2008
  6. Metzker, M., “Sequencing technologies — the next generation”, Nature Rev. Genet, vol. 11, pp. 31–46,2010
  7. Holley, R.W., “Structure of a ribonucleic acid”, Science, vol. 147, pp. 1462–1465, 1965
  8. Chidgeavadze, Z., Beabealashvilli, R.S., “2′, 3′-Dideoxy-3'aminonucleoside 5′-triphosphates are the terminators of DNA synthesis catalyzed by DNA polymerases”, Nucleic Acids Res, vol. 12, pp. 1671–1686, 1984
  9. Artuso, R., Fallerini, C., Dosa, L., Scionti, F., Clementi, M., et al, “Advances in Alport syndrome diagnosis using next-generation sequencing”, Eur. J. Hum. Genet, vol. 20, pp. 50-57, 2012
  10. Anderson,S., “Shotgun DNA sequencing using cloned DNase I-generated fragments”, Nucleic Acids Res, 1981;9:3015–3027
  11. Venter, J. C., Adams, M.D., Myers, E.W., Li, P.W., Mural, R.J., et al, “The sequence of the human genome”, Science, vol. 291, pp. 1304–1351, 2001
  12. Nyrén, P.l., Lundin, A., “Enzymatic method for continuous monitoring of inorganic pyrophosphate synthesis”, Anal. Biochem, vol. 509, pp. 504–509, 1985
  13. Margulies, M., Egholm, M., Altman, W., Attiya, S., “Genome sequencing in microfabricated high-density picolitre reactors”, Nature, vol. 437, pp. 376–380, 2005
  14. Voelkerding, K.V., Dames, S.A., Durtschi, J.D., “Next-generation sequencing: from basic research to diagnostics”, Clin. Chem, vol. 55, pp. 641–658, 2009
  15. Heather, J.M., Chain, B., “The sequence of sequencers: The history of sequencing DNA”, Genomics, vol. 107, pp. 1-8, 2016
  16. Schuster, S.C., “Next-generation sequencing transforms today's biology”, Nat. Methods, vol. 5, pp. 16-18, 2008
  17. Glenn, T.C., “Field guide to next-generation DNA sequencers”, Mol. Ecol. Resour, vol. 11, pp. 759–769, 2011
  18. Rothberg, J.M., “An integrated semiconductor device enabling non-optical genome sequencing”, Nature, vol. 475, pp. 348–352, 2011
  19. Loman, N.J., “Performance comparison of benchtop high-throughput sequencing platforms”, Nat. Biotechnol., vol. 30:pp. 434–439, 2012
  20. Greenleaf, W.J., Sidow, A., “The future of sequencing:convergence of intelligent design and market Darwinism”, Genome Biol., vol. 15, pp. 303, 2014
  21. Schadt, E.E., Turner, S., Kasarskis, A., “A window into third-generation sequencing”, Hum. Mol. Genet., vol. 19, pp. 227–240, 2010
  22. Bowers, J., “Virtual terminator nucleotides for next-generation DNA sequencing” Nat. Methods., vol. 6, pp. 593–595, 2009
  23. Pareek, C.S., Smoczynski, R., “Tretyn A. Sequencing technologies and genome sequencing”, J. Appl. Genet., vol. 52, pp. 413–435, 2011
  24. Kasianowicz, J.J., Brandin, E., Branton, D., Deamer, D.W., “Characterization of individual polynucleotide molecules using a membrane channel”, Proc. Natl. Acad. Sci. U.S.A., vol. 93:pp. 13770–13773,1996
  25. Grada, A., Weinbrecht K., “Next-generation sequencing: methodology and application”, J. Invest. Dermatol., vol. 133, pp. e11, 2013
  26. Pop, M., “Genome assembly reborn: recent computational challenges”, Brief. Bioinform., vol. 10, pp. 354-366, 2009
  27. Ng, S.B., Turner, E.H., Robertson, P.D., Flygare, S.D., Bigham, A.W., et al. “Targeted capture and massively parallel sequencing of 12 human exomes”, Nature, vol. 461, pp. 272–276, 2009
  28. Chilamakuri, C.S.R., Lorenz, S., Madoui, M.A., Vodak, D., Sun, J.C., et al., “Performance comparison of four exome capture systems for deep sequencing”, BMC Genomics, vol. 15, pp. 449, 2014
  29. Rabbani, B., Tekin, M., Mahdieh, N., “The promise of whole-exome sequencing in medical genetics” J Hum Genet., vol. 59, pp. 5–15, 2014
  30. Mamanova, L., Coffey, A.J., Scott, C.E., Kozarewa, I., Turner, E.H., et al., “Target-enrichment strategies for next-generation sequencing”, Nat Methods, vol. 7, pp. 111–118, 2010
  31. Stoddard, J.L., Niemela, J.E., Fleisher T.A., Rosenzweig S.D., “Targeted NGS: A cost-effective approach to molecular diagnosis of PIDs”, Front. Immunol., vol. 5, pp.531, 2014
  32. Ozsolak, F., Milos, P.M., “RNA sequencing: Advances, challenges and opportunities” Nat Rev Genet., vol. 12, pp. 87–98, 2011
  33. Gilbert, J.A., Dupont, C.L., Microbial metagenomics: Beyond the genome” Annu Rev Mar Sci., vol. 3, pp. 347–371, 2011
  34. Weinstock, G.M., “Genomic approaches to studying the human microbiota”, Nature., vol. 489, pp. 250–256, 2012
  35. Wylie, K.M., Weinstock, G.M., Storch, G.A., “Virome genomics:A tool for defining the human virome” Curr Opin Microbiol., vol. 16, pp. 479–484, 2013
  36. Ursell, L.K., Metcalf, J.L., Parfrey, L.W., Knight, R.,”Defining the human microbiome”, Nutr Rev., vol. 70, pp. 38–44, 2012 [37] Peter, A.J., Stephen, B.B., “The Epigenomics of Cancer”, Cell., vol. 128, pp. 683-692, 2007
  37. Hirst, M., “Epigenomics: Sequencing the methylome”, Methods Mol Biol., vol. 973, pp. 39– 54, 2013
  38. Esteller, M., “Cancer epigenomics: DNA methylomes and histone-modification maps”, Nat Rev Genet., vol. 8, pp. 286-298, 2007
  39. Wion, D., Casadesus, J., “N6-methyl-adenine: an epigenetic signal for DNA-protein interactions” Nature Rev. Microbiol., vol. 4, pp. 183–192, 2006
  40. Merlo, A., Herman, J.G., Mao,L., Lee, D.J., Gabrielson, E., et al., “5' CpG island methylation is associated with transcriptional silencing of the tumour suppressor p16/CDKN2/MTS1 in human cancers” Nature Med., vol. 1, pp. 686–692, 1995
  41. Vijayakumar, N.T., Judy, M.V., “Autism spectrum disorders: Integration of the genome, transcriptome and the environment”, J Neurol Sci. vol. 364, pp. 167-176, 2016
  42. Heather, J.M., Chain, B. “The sequence of sequencers: The history of sequencing DNA”, Genomics, vol. 107, pp. 1–8, 2016
  43. Fiers, W., Contreras, R., Duerinck, F., Haegeman, G., Iserentant, D., et al., “Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene”, Nature, vol. 260, pp. 500–507, 1976
  44. Maxam, A.M., Gilbert, W., “A new method for sequencing DNA”, Proc Natl Acad Sci USA, vol. 74, pp. 560 – 564, 1977
  45. Sanger, F., Nicklen, S., Coulson, A.R., “DNA sequencing with chain-terminating inhibitors”, Proc Natl Acad Sci, vol. 74, pp. 5463−5467, 1977
  46. Mardis, E.R., “The impact of next-generation sequencing technology on genetics”, Trends in Genetics, vol. 24, pp. 133-141, 2008
  47. Metzker, M., “Sequencing technologies — the next generation”, Nature Rev. Genet, vol. 11, pp. 31–46,2010
  48. Holley, R.W., “Structure of a ribonucleic acid”, Science, vol. 147, pp. 1462–1465, 1965
  49. Chidgeavadze, Z., Beabealashvilli, R.S., “2′, 3′-Dideoxy-3'aminonucleoside 5′-triphosphates are the terminators of DNA synthesis catalyzed by DNA polymerases”, Nucleic Acids Res, vol. 12, pp. 1671–1686, 1984
  50. Artuso, R., Fallerini, C., Dosa, L., Scionti, F., Clementi, M., et al, “Advances in Alport syndrome diagnosis using next-generation sequencing”, Eur. J. Hum. Genet, vol. 20, pp. 50-57, 2012
  51. Anderson,S., “Shotgun DNA sequencing using cloned DNase I-generated fragments”, Nucleic Acids Res, 1981;9:3015–3027
  52. Venter, J. C., Adams, M.D., Myers, E.W., Li, P.W., Mural, R.J., et al, “The sequence of the human genome”, Science, vol. 291, pp. 1304–1351, 2001
  53. Nyrén, P.l., Lundin, A., “Enzymatic method for continuous monitoring of inorganic pyrophosphate synthesis”, Anal. Biochem, vol. 509, pp. 504–509, 1985
  54. Margulies, M., Egholm, M., Altman, W., Attiya, S., “Genome sequencing in microfabricated high-density picolitre reactors”, Nature, vol. 437, pp. 376–380, 2005
  55. Voelkerding, K.V., Dames, S.A., Durtschi, J.D., “Next-generation sequencing: from basic research to diagnostics”, Clin. Chem, vol. 55, pp. 641–658, 2009
  56. Heather, J.M., Chain, B., “The sequence of sequencers: The history of sequencing DNA”, Genomics, vol. 107, pp. 1-8, 2016
  57. Schuster, S.C., “Next-generation sequencing transforms today's biology”, Nat. Methods, vol. 5, pp. 16-18, 2008
  58. Glenn, T.C., “Field guide to next-generation DNA sequencers”, Mol. Ecol. Resour, vol. 11, pp. 759–769, 2011
  59. Rothberg, J.M., “An integrated semiconductor device enabling non-optical genome sequencing”, Nature, vol. 475, pp. 348–352, 2011
  60. Loman, N.J., “Performance comparison of benchtop high-throughput sequencing platforms”, Nat. Biotechnol., vol. 30:pp. 434–439, 2012
  61. Greenleaf, W.J., Sidow, A., “The future of sequencing:convergence of intelligent design and market Darwinism”, Genome Biol., vol. 15, pp. 303, 2014
  62. Schadt, E.E., Turner, S., Kasarskis, A., “A window into third-generation sequencing”, Hum. Mol. Genet., vol. 19, pp. 227–240, 2010
  63. Bowers, J., “Virtual terminator nucleotides for next-generation DNA sequencing” Nat. Methods., vol. 6, pp. 593–595, 2009
  64. Pareek, C.S., Smoczynski, R., “Tretyn A. Sequencing technologies and genome sequencing”, J. Appl. Genet., vol. 52, pp. 413–435, 2011
  65. Kasianowicz, J.J., Brandin, E., Branton, D., Deamer, D.W., “Characterization of individual polynucleotide molecules using a membrane channel”, Proc. Natl. Acad. Sci. U.S.A., vol. 93:pp. 13770–13773,1996
  66. Grada, A., Weinbrecht K., “Next-generation sequencing: methodology and application”, J. Invest. Dermatol., vol. 133, pp. e11, 2013
  67. Pop, M., “Genome assembly reborn: recent computational challenges”, Brief. Bioinform., vol. 10, pp. 354-366, 2009
  68. Ng, S.B., Turner, E.H., Robertson, P.D., Flygare, S.D., Bigham, A.W., et al. “Targeted capture and massively parallel sequencing of 12 human exomes”, Nature, vol. 461, pp. 272–276, 2009
  69. Chilamakuri, C.S.R., Lorenz, S., Madoui, M.A., Vodak, D., Sun, J.C., et al., “Performance comparison of four exome capture systems for deep sequencing”, BMC Genomics, vol. 15, pp. 449, 2014
  70. Rabbani, B., Tekin, M., Mahdieh, N., “The promise of whole-exome sequencing in medical genetics” J Hum Genet., vol. 59, pp. 5–15, 2014
  71. Mamanova, L., Coffey, A.J., Scott, C.E., Kozarewa, I., Turner, E.H., et al., “Target-enrichment strategies for next-generation sequencing”, Nat Methods, vol. 7, pp. 111–118, 2010
  72. Stoddard, J.L., Niemela, J.E., Fleisher T.A., Rosenzweig S.D., “Targeted NGS: A cost-effective approach to molecular diagnosis of PIDs”, Front. Immunol., vol. 5, pp.531, 2014
  73. Ozsolak, F., Milos, P.M., “RNA sequencing: Advances, challenges and opportunities” Nat Rev Genet., vol. 12, pp. 87–98, 2011
  74. Gilbert, J.A., Dupont, C.L., Microbial metagenomics: Beyond the genome” Annu Rev Mar Sci., vol. 3, pp. 347–371, 2011
  75. Weinstock, G.M., “Genomic approaches to studying the human microbiota”, Nature., vol. 489, pp. 250–256, 2012
  76. Wylie, K.M., Weinstock, G.M., Storch, G.A., “Virome genomics:A tool for defining the human virome” Curr Opin Microbiol., vol. 16, pp. 479–484, 2013
  77. Ursell, L.K., Metcalf, J.L., Parfrey, L.W., Knight, R.,”Defining the human microbiome”, Nutr Rev., vol. 70, pp. 38–44, 2012 [37] Peter, A.J., Stephen, B.B., “The Epigenomics of Cancer”, Cell., vol. 128, pp. 683-692, 2007
  78. Hirst, M., “Epigenomics: Sequencing the methylome”, Methods Mol Biol., vol. 973, pp. 39– 54, 2013
  79. Esteller, M., “Cancer epigenomics: DNA methylomes and histone-modification maps”, Nat Rev Genet., vol. 8, pp. 286-298, 2007
  80. Wion, D., Casadesus, J., “N6-methyl-adenine: an epigenetic signal for DNA-protein interactions” Nature Rev. Microbiol., vol. 4, pp. 183–192, 2006
  81. Merlo, A., Herman, J.G., Mao,L., Lee, D.J., Gabrielson, E., et al., “5' CpG island methylation is associated with transcriptional silencing of the tumour suppressor p16/CDKN2/MTS1 in human cancers” Nature Med., vol. 1, pp. 686–692, 1995
  82. Vijayakumar, N.T., Judy, M.V., “Autism spectrum disorders: Integration of the genome, transcriptome and the environment”, J Neurol Sci. vol. 364, pp. 167-176, 2016.