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Journals / Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2023 / Cilt: 6 Sayı: 2

Cloning, Expression, and Purification of Acinetobacter baumannii OccAB1 Porin Protein in Escherichia coli

Escherichia coli'de Acinetobacter baumannii OccAB1 Porin Proteininin Klonlanması, Ekspresyonu ve Saflaştırılması

Özge KAYGUSUZ İZGÖRDÜ
Cihan DARCAN
Fatma DOĞAN GUZEL
Pages
1529–1542
Publication Date
2023-07-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result, A. baumannii OccAB1 porin cloned into the expression vector was successfully expressed in E. coli.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Acinetobacter baumannii is a free-living gram-negative organism in different habitats such as soil, water, surfaces of human and animal bodies.
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Key Findings & Data:In this study, A. baumannii OccAB1 porin protein was cloned and expressed in E. coli.
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Conclusion & Impact:As a result, A. baumannii OccAB1 porin cloned into the expression vector was successfully expressed in E. coli.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:E. coli · A. baumannii · Ni-NTA

Abstract

Acinetobacter baumannii is a free-living gram-negative organism in different habitats such as soil, water, surfaces of human and animal bodies. However, adapting to a pathogenic lifestyle over time, it causes infections and deaths in intensive care units all over the world. Porin proteins play a fundamental role in antibiotic resistance in this bacterium. Overexpression of porins and efflux pumps have been identified as contributing factors in increasing drug resistance and inactivation of therapy. For this reason, to study antibiotic transitions in vitro, these proteins must be purified first. With this porin protein obtained after purification, the passage of antibiotics in bilayer lipid layers will be investigated. In this study, A. baumannii OccAB1 porin protein was cloned and expressed in E. coli. As a result, A. baumannii OccAB1 porin cloned into the expression vector was successfully expressed in E. coli.

Özet

Acinetobacter baumannii, toprakta, suda, insan ve hayvan vücutlarının yüzeyleri gibi farklı habitatlarda serbest yaşayan gram negatif bir organizmalardır. Ancak zamanla patojenik bir yaşam tarzına adapte olarak tüm dünyada yoğun bakım ünitelerinde enfeksiyonlara ve ölümlere neden olmaktadır. Antibiyotik direncinde porin proteinleri bu bakteride temel rol oynamaktadır. Porinler ve dışa akış pompalarının aşırı ekspresyonu, ilaç direncini artırmada ve tedavinin etkisiz hale getirilmesinde katkıda bulunan faktörler olarak tespit edilmiştir. Bu sebeple antibiyotik geçişlerinin in vitro olarak çalışılabilmesi için öncelikli olarak bu proteinlerin saflaştırılması gerekmektedir. Saflaştırma işleminin ardından elde edilen bu porin proteini ile çift katmanlı lipit tabakalarda antibiyotiklerin geçişleri araştırılacaktır. Bu çalışmada, A. baumannii OccAB1 porin proteini klonlandı ve E. coli'de eksprese edildi. Sonuç olarak, ekspresyon vektörüne klonlanan A. baumannii OccAB1 porini, E. coli'de başarıyla eksprese edildi.

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Cite This Article

İZGÖRDÜ, Ö. K., DARCAN, C., GUZEL, F. D. (2023). Cloning, Expression, and Purification of Acinetobacter baumannii OccAB1 Porin Protein in Escherichia coli. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 6 Sayı: 2, 1529–1542. https://ajindex.com/articles/952762

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