SavedAdd your journal

Journals / Cumhuriyet Üniv. Tıp Fak. Derg. / 2003 / Cilt: 25 - Sayı: 4

Human placental glutathione-S-transferase: Isolation, kinetic properties and in vitro interactions with several drugs

İnsan plasental glutatyon S-transferazının izolasyonu, kinetik özellikleri ve bazı ilaçlarla in vitro etkileşimi

V. Kenan ÇELİK
Ferah ARMUTCU
Ahmet AKER
Pages
187–192
Publication Date
2022-10-10
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results suggested that these drugs must be used carefully in pregnant women.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Placental GST-n was isolated from full-term placenta by glutathione affinity column.
📊
Key Findings & Data:GST was obtained with 0,5 % yield and had a specific activity of 2628 units per milligram protein.
💡
Conclusion & Impact:These results suggested that these drugs must be used carefully in pregnant women.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Placental GST-n was isolated from full-term placenta by glutathione affinity column. GST was obtained with 0,5 % yield and had a specific activity of 2628 units per milligram protein. Km and Vmax values for glutathione (GSH) and chloro-2,4-dinitrobenzen (CDNB) as substrates were 2mM, 11,1 U/mg min and 0,55 mM, 35,0 U/mg min respectively. it was observed that GST activity was decreased 50-55% by Pre-Par® (Ritodrine HCL) used to prevent the premature birth. Metpamid® (Metoclopramid), an antiemetic, inhibited the enzyme activity 80-85%. Novalgine® (Metamizole), known as an analgesic and antipyretic, is also inhibited enzyme activity 70-80%.In addition to these drugs Sultacide® (Sultamisilin) and lesef® (Ceftriaxon) which have an antibacterial effect inhibited the enzyme activity 10-20%. These results suggested that these drugs must be used carefully in pregnant women.

Özet

Plasental glutatyon-S-transferaz (GST-n) enzimi myiadında doğum yapmış olgunun plasentasından glutatyon agaroz affinite kolonu kullanılarak izole edildi. GST-n 2628 umol/mg dk spesifik aktivite ve % 0,5 verimle saflaştırıldı. Enzimin substratı olan glutatyon ( GSH ) için Km değeri 2 mM, Vmax değeri 11.1 U/mg dk; kloro-2,4-dinitrobenzen ( CDNB ) için Km değeri 0,55mM, Vmax değeri 35.0 U/mg dk olarak bulundu. İlaç etkileşimi çalışmalarında, prematüre doğumların önlenmesinde kullanılan Pre-Par® (Ritodrin HCL) ve antibakteriyel etkili Ampisid® (Ampisilin), enzim aktivitesini in vitro olarak % 50-55 oranında inhibe ettiği gözlendi. Antiemetik olarak kullanılan Metpamid® (Metoclopramid) enzim aktivitesini %80-85 oranında inhibe ederken, klinik kullanımı oldukça yaygın olan, ağrı kesici ve ateş düşürücü etkili Novaljin® (Metamizol), enzim aktivitesini % 70-80 oranında inhibe etti.Yine anti-bakteriyel etkileri olan lesef® (Ceftriaxon) ve Sultasid® (Sultamisilin)'in .ise enzim aktivitesini %10-15 oranında inhibe ettiği gözlendi. Bu bulgular bu tür ilaçların hamilelerde çok titiz ve dikkatli bir seçimle kullanılması gereğini ortaya koymaktadır.

🔖

Cite This Article

ÇELİK, V. K., ARMUTCU, F., AKER, A. (2022). Human placental glutathione-S-transferase: Isolation, kinetic properties and in vitro interactions with several drugs. Cumhuriyet Üniv. Tıp Fak. Derg., Cilt: 25 - Sayı: 4, 187–192. https://ajindex.com/articles/480188

References (1)

  1. Ketterer, B.: Detoxication reactions of glutathione and glutathione transferases. Xenobiotica. 1986;16 (10-11): 957-973, 2. Vermeulen, N.P.E.: Analysis of mercapturic acids as a tool in biotransformation, biomonitoring and toxicological studies. “Glutathione-S-transferases and Drug Resistance” Taylor and Francis London 1990: 1,141-153,. 3. Snel, C.A.W. Zhao, Y. Mulder, G.J. Pang, K.S.: Methods for the quantitation of bromosulfophthalein and its glutathione conjugate in biological fluids. Anal. Biochem 1993; 212: 28-34. 4. Zhao, Y. Snel. C.A.W. Mulder, G.J. and Pang, K.S.: Localization of glutathione conjugation activities toword bromosulfophthalein in perfused rat Liver: Studies with the multiple indicator dilution technique. Drug Metab. Dispos. 1993; 21: 1070-1078. 5. Mannervik,B.Guthenberg,C.:Glutathionetransferase (Human placenta).Methods in Enzymology. 1981; 77 (28):231-235. 6. Mannervik,B. Awasthi Y.C. Board, P.G et al.: Nomenculature for human glutathione transferases. Biochem J 1992; 282: 305-308, 7. Sato, K. Kitahara, A. Satoh, K. Ishikawa, T. Tatematsu, M. Ito, N.: The placental form of glutathione S - transferase as a new marker protein for preneoplasia in rat chemical hepatocarcinogenesis. Jpn J. Cancer Res 1984;75:199-202. 8. Tatematsu, M.Mera,Y. Ito, N. Satoh, K. Sato, K.: Relative merits of immunohistochemical demonstration of placsntal A,B and C forms of glutathione S-transferase and histochemical demonstration of γ-glutamyl transferase as markers of altared foci during liver carcinogenesis in rats. Carcinogenesis. 1985; 6: 1621-1626. 9. Kodate,C. Fukushı,A. Narıta,T. Kuda,H. Soma,Y.and Sato, K.: Human placental form of glutathione-S-transferase (GST-p) as a new ımmunohıs tochemical marker for human colonic carcinoma. Jpn. J. Cancer Res. 1986; 77: 226-229, 10. Shiratori, Y. Soma, Y. Maruyama, H. Sato, S. Takano, A and Sato, K.: Immunohistochemical detection of the planceltal form of glutathione-S-transferase in dysplastic and neoplastic human uterine cervix lesions. Cancer research. 1987; 47: 6808-6809. 11. Harrison,DJ.Khatbanda,R. Bishop,D. McLelland,LI. Hayes, JD.:GlutathioneS-transferases in human renal carcinoma demonstrated by immunohistochemistry. Carcinogenesis. 1989;10:1257-1260. 12. Pasanen,M.Pelkonen,O.:Xenobiotic and steroid metabolizing monooksigenases catalysed by cytochrome P450 and glutathione S-transferase conjugations in human placenta and their relationships to maternal cigarette smoking. Placenta 1990;11:75