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Journals / Bornova Veteriner Bilimleri Dergisi / 2011 / Cilt: 33 - Sayı: 47

The investigation of tdh and trh genes of Vibrio parahaemolyticus isolates from bivalve molluscs with multiplex polimerase chain reaction

Çift kabuklu yumuşakçalardan izole edilen Vibrio parahaemolyticus izolatlarının tdh ve trh genlerinin multiplex polimeraz zincir reaksiyonu yöntemi ile araştırılması

Necla TÜRK
Meriç Lütfi AVSEVER
Cemal ÜN
Pages
29–36
Publication Date
2022-10-11
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In addition, all of the 34 isolates obtained were urea negative and 2 isolates were Kanagawa Phenomenon positive.

📌 3 Key Takeaways

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Objective & Hypothesis:In this work, 34 Vibrio parahaemolyticus isolates obtained from bivalve molluscs (wedge shell-Donax trunculus, L. 1758, oyster-Ostrea edulis, L. 1758, cockle-Venus verrucosa, L. 1758, clam-Tapes decussatus, L. 1758, bearded mussel-Modiolus barbatus, L. 1758, Mediterranean mussel- Mytilus galloprovincialis L. 1819, venus-Chamelea gallina L., 1753) production stations were used.
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Key Findings & Data:All of the 34 V. parahaemolyticus isolates identified according to TSE/ISO 8914 were found to be negative for tdh (thermostable direct haemolysin gene) and trh (thermostable related haemolysin gene) genes and positive for a specific gene region for V. parahaemolyticus (R72H) after application of multiplex PCR (mPCR) method.
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Conclusion & Impact:In addition, all of the 34 isolates obtained were urea negative and 2 isolates were Kanagawa Phenomenon positive.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this work, 34 Vibrio parahaemolyticus isolates obtained from bivalve molluscs (wedge shell-Donax trunculus, L. 1758, oyster-Ostrea edulis, L. 1758, cockle-Venus verrucosa, L. 1758, clam-Tapes decussatus, L. 1758, bearded mussel-Modiolus barbatus, L. 1758, Mediterranean mussel- Mytilus galloprovincialis L. 1819, venus-Chamelea gallina L., 1753) production stations were used. All of the 34 V. parahaemolyticus isolates identified according to TSE/ISO 8914 were found to be negative for tdh (thermostable direct haemolysin gene) and trh (thermostable related haemolysin gene) genes and positive for a specific gene region for V. parahaemolyticus (R72H) after application of multiplex PCR (mPCR) method. In addition, all of the 34 isolates obtained were urea negative and 2 isolates were Kanagawa Phenomenon positive.

Özet

Bu çalışmada, üretim istasyonlarındaki çift kabuklu yumuşakçalardan (kum şırlanı-Donax trunculus, L. 1758; yassı istiridye-Ostrea edulis, L. 1758; kidonya-Venus verrucosa, L. 1758; akivades- Tapes decussatus, L. 1758; kıllı midye- Modiolus barbatus, L. 1758; kara midye- Mytilus galloprovincialis, L. 1819; kum midyesi- Chamelea gallina L., 1753) izole edilen 34 Vibrio parahaemolyticus izolatı kullanıldı. TSE/ISO 8914’e göre V. parahaemolyticus olarak identifiye edilen 34 izolatın tamamının tdh ve trh genleri yönünden negatif olduğu, V. parahaemolyticus’a spesifik gen bölgesi (R72H) yönünden ise pozitif olduğu bulundu. Ayrıca, 34 izolatın üre hidrolizi negatif bulunurken, 2 izolatın Kanagawa Fenomeni pozitif bulunmuştur.

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Cite This Article

TÜRK, N., AVSEVER, M. L., ÜN, C. (2022). The investigation of tdh and trh genes of Vibrio parahaemolyticus isolates from bivalve molluscs with multiplex polimerase chain reaction. Bornova Veteriner Bilimleri Dergisi, Cilt: 33 - Sayı: 47, 29–36. https://ajindex.com/articles/467818

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