Effects of Panax ginseng on cisplatin-induced testicular damage of rats

Objective & Hypothesis:Infertility has become an increasingly important health problem due to genetic, familial, hormonal, and congenital abnormalities, environmental and chemical reasons.

📊

Key Findings & Data:Four animal groups were applied with different protocols as Control, Pnx (200 mg / kg), CP (7 mg / kg), and CP + Pnx (200 mg / kg).

💡

Conclusion & Impact:According to the results, free/total testosterone and TAC levels were decreased while TOS levels increased in injured rats' plasma.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Cisplatin · Panax ginseng · Apoptoz

Abstract

Infertility has become an increasingly important health problem due to genetic, familial, hormonal, and congenital abnormalities, environmental and chemical reasons. This study aimed to investigate the effects of Panax ginseng (Pnx) root extract on cisplatin (CP) induced testicular damage of rats. Four animal groups were applied with different protocols as Control, Pnx (200 mg / kg), CP (7 mg / kg), and CP + Pnx (200 mg / kg). At the end of the experiment, the body and testicular weights of the rats were measured. While free/total testosterone, total antioxidant capacity (TAC), and total oxidative species (TOS) levels were analyzed in blood samples, apoptotic cells were marked by TUNEL staining in testicular samples of rats. According to the results, free/total testosterone and TAC levels were decreased while TOS levels increased in injured rats' plasma. On the other hand, seminiferous tubule diameters widened, and the number of apoptotic cells increased in rats' testis. These variables were significantly improved with the consumption of Pnx. As a result, Pnx has a significant protective effect on testicular tissue; however, further studies are needed to elucidate its action mechanism.

Özet

İnfertilite genetik, ailevi, hormonal nedenler, doğumsal anormallikler, çevresel ve kimyasal nedenlerle günümüzde artmakta olan önemli bir sağlık sorunu haline gelmiştir. Bu çalışma ile sıçanlarda sisplatin (CP) ile indüklenen testis hasarı üzerine Panax ginseng (Pnx) kök ekstraktının etkilerinin incelenmesi amaçlanmıştır. Sıçanlar Kontrol, Pnx (200 mg/kg), CP (7 mg/kg) ve CP+Pnx (200 mg/kg) olmak üzere 4 gruba ayrılmıştır. Deney sonunda sıçanların vücut ve testis ağırlıkları ölçülmüştür. Sıçanların kan örneklerinde serbest/total testosteron, toplam antioksidan kapasite (TAK) ve toplam oksidan durum (TOD) seviyeleri analiz edilirken, testis örneklerinde TUNEL boyaması ile apoptotik hücreler işaretlenmiştir. Sonuçlara göre hasar oluşturulmuş sıçanların plazma örneklerinde serbest/total testosteron ve TAK düzeyleri azalırken, TOD miktarı artmıştır. Testis dokularında seminifer tübül çapları genişlemiş ve apoptotik hücre sayısı artmıştır. Pnx uygulaması ile bu değişkenlerin ciddi oranda düzeldiği saptanmıştır. Sonuç olarak, Pnx’in testiküler dokuda belirgin koruyucu etkinliği bulunmaktadır; fakat etki mekanizmasının aydınlatılabilmesi için ileri çalışmalara gereksinim vardır.

Keywords

Cisplatin Panax ginseng Apoptoz TAK TOD Apoptosis TAC TOS

🔖

Cite This Article

ASLAN, E., KUMALAR, K., GÜZEL, H., DEMİREL, H. H., ÇELİK, S., PEKTAŞ, M. B. (2021). Effects of Panax ginseng on cisplatin-induced testicular damage of rats. Anatolian Journal of Botany, Cilt: 5 - Sayı: 1, 37–43. https://ajindex.com/articles/461760

📄 Full Text PDF Viewer

Open in New Tab ↗

↗New Tab

📄

PDF görüntüleyici hazırlanıyor...

References (13)

Aksu EH, Kandemir FM, Altun S, Küçükler S, Çomaklı S, Ömür AD (2016). Ameliorative effect of carvacrol on cisplatin-induced reproductive damage in male rats. Journal of Biochemical and Molecular Toxicology 30(10): 513-520.
Aly HAA, Eid BG (2020). Cisplatin-induced testicular damage through mitochondria-mediated apoptosis, inflammation and oxidative stress in rats: Impact of resveratrol. Endocrine Journal 67(9): 969-980.
Azab SS, Kamel I, Ismai, NN, El Din Hosni H, El Fatah MA (2020). The defensive role of taurine against gonadotoxicity and testicular apoptosis effects induced by cisplatin in rats. Journal of Infection and Chemotherapy 26(1): 51-57.
Barabas K, Milner R, Lurie D, Adin C (2008). Cisplatin: A review of toxicities and therapeutic applications. Veterinary and Comparative Oncology 6(1): 1-18.
Ceylan T, Kaymak E, Cantürk F, Yakan B (2020). Research on the protective effect of caffeic acid phenethyl ester on testicular damage caused by cisplatin. Turkish Journal of Medical Sciences 50(8): 2032-2039.
Dasari S, Bernard TP (2014). Cisplatin in cancer therapy: Molecular mechanisms of action. European Journal of Pharmacology 740: 364-378.
De Freitas AT, Figueiredo PC, de Aquino AM, Fernandes AH, Fantin DR, Justulin LA, Scarano WR (2019). Panax ginseng metabolite (GIM-1) prevents oxidative stress and apoptosis in human Sertoli cells exposed to Monobutyl-phthalate (MBP). Reproductive Toxicology 86: 68-75.
Erel O (2004). A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical Biochemistry 37(2): 112-119.
Erel O (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry 38(12): 1103-1111.
Famurewa AC, Ekeleme-Egedigwe CA, Onwe CS, Egedigwe UO, Okoro CO, Egedigwe UJ, Asogwa NT (2020). Ginger juice prevents cisplatin-induced oxidative stress, endocrine imbalance and NO/iNOS/NF-κB signalling via modulating testicular redox-inflammatory mechanism in rats. Andrologia 52(10): 1-10.
Geyikoğlu F, Çolak S, Türkez H, Bakır M, Koç K, Hosseinigouzdagani MK, Çeriğ S, Sönmez M (2017). Oleuropein ameliorates cisplatin-induced hematological damages via restraining oxidative stress and DNA injury. Indian Journal of Hematology and Blood Transfusion 33(3): 348-354.
Hanigan MH, Devarajan P (2003). Cisplatin nephrotoxicity: molecular mechanisms. Cancer Therapy 1: 47-61.
Johnsen SG (1970). Testicular biopsy score count – A method for registration of spermatogenesis in human testes: