AI Summary & Key Takeaways
AI InsightAs a result, it was found that; i) the erythrocyte arginase activities of healthy cows were markedly higher (P
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Abstract
This study was conducted to establish the erythrocyte arginase activity and plasma nitric oxide levels in healthy cows and cows with pyometra.The study material consisted of a total of 20 cows, aged between 4 and 8 years old and in various breeds, admitted to the Obstetrics and Gynaecology Clinic, Animal Hospital, Faculty of Veterinary Medicine, Fırat University. Half of the animals were diagnosed as clinically healthy, while the others were diagnosed with pyometra. Following the centrifugation of blood samples collected from V. Jugularis into heparin‐filled tubes, the plasma nitric oxide levels were measured. Arginase activity was measured in erythrocytes after washing up of the remaining portion 3 times with serum physiologic. The activity was measured spectrophotometrically with the Thiosemicarbazide‐Diacetyl Monoxime Urea (TDMU) method. Drabkin method was used to measure the haemoglobin amount, while enzymatic Griess method was used to measure the nitric oxide levels. As a result, it was found that; i) the erythrocyte arginase activities of healthy cows were markedly higher (P
Özet
Bu çalışma sağlıklı ve piyometralı ineklerde eritrosit arginaz aktivitesi ile plazma nitrik oksit düzeylerini saptamak amacı ile yapıldı. Araştırma materyalini Fırat Üniversitesi Veteriner Fakültesi Hayvan Hastanesi Doğum ve Jinekoloji Kliniğine getirilen 10 sağlıklı ve 10 klinik olarak piyometra teşhisi konulan, değişik ırklardan, 4‐8 yaşlarında toplam 20 inek oluşturdu. İneklerin V. Jugularisten heparinli tüplere alınan kan örnekleri santrifüj edildikten sonra plazmasında nitrik oksit düzeyi, geriye kalan kısmın 3 defa serum fizyolojik ile yıkanmasından sonra elde edilen eritrositlerde de arginaz aktivitesine bakıldı. Arginaz aktivitesi Tiyosemikarbazid‐Diasetil Monoksim Üre (TDMU) metodu ile spektrofotometrik olarak ölçüldü. Hemoglobin miktarını tespit etmede Drabkin metodu, nitrik oksit düzeyi ölçmede ise enzimatik Griess yöntemi kullanıldı. Sonuç olarak sağlıklı ineklerin eritrosit arginaz aktivitelerinin piyometralı ineklere göre belirgin derecede fazla olduğu (p