Journals / Atatürk Üniversitesi Veteriner Bilimleri Dergisi / 2011 / Cilt: 6 - Sayı: 3

An immunohistochemical study on the gastrin-, somatostatin- and serotonin-releasing cells in the gastrointestinal system of adult quails

Yetişkin bıldırcınların gastrointestinal sisteminde gastrin, somatostatin ve serotonin salgılayan hücreler üzerine immunohistokimyasal bir araştırma

Pages
183–193
Publication Date
2022-10-11
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Also, the G and EC cells were observed numerously in the mucosal membranes of duodenum and ileum, as compared to that of D cells.

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Objective & Hypothesis:In this research, we aimed to determine the mucosal localisation and frequency of immune positive cells of gastrin (G cells), somatostatin (D cells) and serotonin (EC cells) in gastrointestinal tract of adult quails using immunohisto-chemical staining methods.
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Key Findings & Data:For this purpose, proventriculus, gizzard, duodenum, jejunum and ileum of 10 healthy adult quails were used as materials.
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Conclusion & Impact:Also, the G and EC cells were observed numerously in the mucosal membranes of duodenum and ileum, as compared to that of D cells.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this research, we aimed to determine the mucosal localisation and frequency of immune positive cells of gastrin (G cells), somatostatin (D cells) and serotonin (EC cells) in gastrointestinal tract of adult quails using immunohisto-chemical staining methods. For this purpose, proventriculus, gizzard, duodenum, jejunum and ileum of 10 healthy adult quails were used as materials. Tissue sections taken were stained immunohistochemically by streptavidin biotin peroxidase method. Gastrin- and serotonin-immunopositive cells were determined to be abundant in small intestines, as compared to those of proventriculus and gizzard. However, somatostatin immunopositive cells were observed in a few numbers in stomach and all segments of small intestines. Moreover, the G, D and EC cells were also demonstrated usually to be in closed type in the proventriculus and gizzard, but in both open and closed types in all segments of small intestines. As a result, the G, D and EC cells were determined in varying numbers in the proventriculus, gizzard, duodenum, jejunum and ileum of the quails. Also, the G and EC cells were observed numerously in the mucosal membranes of duodenum and ileum, as compared to that of D cells.

Özet

Bu araştırmada, yetişkin bıldırcınların gastrointestinal sistemindeki gastrin (G hücreleri), somatostatin (D hücreleri) ve serotonin (EC hücreleri) immunopozitif hücrelerin dağılımı, yerleşimi ve sıklığının immunohistokimyasal yöntemlerle belirlenmesi amaçlandı. Çalışmada, 10 adet sağlıklı yetişkin bıldırcının glanduler ve musküler mideleri, duodenum, jejunum ve ileumları materyal olarak kullanıldı. Alınan doku kesitleri streptavidin biotin peroksidaz yöntemi ile immunohistokimyasal olarak boyandı. Gastrin ve serotonin immunopozitif hücrelerin glanduler ve musküler midelere göre ince barsaklarda daha fazla olduğu belirlendi. Bununla birlikte, somatostatin immunopozitif hücreler mide ve ince barsakların tamamında çok az sayıda görüldü. Ayrıca, G, D ve EC hücrelerinin her iki midede genellikle kapalı tip, ince barsak bölümlerinde ise hem açık hem de kapalı tip endokrin hücreler halinde bulunduğu da tespit edildi. Sonuç olarak, bıldırcınların glanduler ve musküler mideleri, duodenum, jejunum ve ileumlarında G, D ve EC hücrelerinin değişen sayılarda bulunduğu belirlendi. Ayrıca, duodenum ve ileum mukozasında, G ve EC hücrelerinin D hücrelerine göre daha fazla bulunduğu da tespit edildi.

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Cite This Article

CAN, İ., KARADENİZ, A., ŞİMŞEK, N., KARA, A., ÖZÜDOĞRU, Z. (2022). An immunohistochemical study on the gastrin-, somatostatin- and serotonin-releasing cells in the gastrointestinal system of adult quails. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, Cilt: 6 - Sayı: 3, 183–193. https://ajindex.com/articles/456080

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