Journals / Atatürk Üniversitesi Veteriner Bilimleri Dergisi / 2014 / Cilt: 9 - Sayı: 3

The Effect of Delaying the Administration of Second GnRH in the Ovsynch Protocol on Pregnancy Rate in Dairy Cows

Sütçü İneklerde Ovsynch Protokolünde İkinci GnRH Uygulamasının Geciktirilmesinin Gebelik Oranı Üzerine Etkisi

Pages
187–193
Publication Date
2022-10-11
DOI
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⚡ TL;DR(Too Long; Didn't Read)

In conclusion, the findings suggestthat delaying the second GnRH injection for 8 hours (56th hour instead of 48th hour) in conventional Ovsynch protocol hadno effect on pregnancy rate in dairy cows.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to evaluate the effect of delaying the second GnRH injection on pregnancy rates in theOvsynch protocol ( OVS) in cows.
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Key Findings & Data:In the study, a total of 40 primiparous, Holstein- Friesian dairy cows treated with 10 μgGnRH on day zero and 500 μg PGF2 α on day 7 and the cows were then randomly divided into two groups.
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Conclusion & Impact:In conclusion, the findings suggestthat delaying the second GnRH injection for 8 hours (56th hour instead of 48th hour) in conventional Ovsynch protocol hadno effect on pregnancy rate in dairy cows.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to evaluate the effect of delaying the second GnRH injection on pregnancy rates in theOvsynch protocol ( OVS) in cows. In the study, a total of 40 primiparous, Holstein- Friesian dairy cows treated with 10 μgGnRH on day zero and 500 μg PGF2 α on day 7 and the cows were then randomly divided into two groups. T he 2nd GnRHinjection was performed at the 48 hours in the OVS 48 (n = 21) and at the 56 hours in the OVS 56 (n = 19) after the PGF2 αinjection. Artificial insemination (AI) was performed 16 hours after the protocols. Blood samples were taken at day 7 and atthe time of insemination to evaluate progesterone ( P 4 ) . Pregnancy diagnosis was determined at 40- 45th day after the AI bytransrectal ultrasonography. No statistically significant differences were determined between groups for the mean serum P 4 values on day 7 and at the time of insemination (OVS 48: 9.11 ± 7.60 and 0.89 ± 1.31 ng/ml vs. OVS 56: 6.00 ± 5.98 and 1.10 ± 1.79 ng/ml, respectively) (P> 0.05). Pregnancy rates were determined as 66.66 % in group OVS 48 and 47.36 % in groupOVS 56. However, no significant difference was observed between the groups (P>0.05). In conclusion, the findings suggestthat delaying the second GnRH injection for 8 hours (56th hour instead of 48th hour) in conventional Ovsynch protocol hadno effect on pregnancy rate in dairy cows.

Özet

Bu çalışmada, ineklerde Ovsynch (OVS) protokolünde ikinci GnRH enjeksiyonunun geciktirilmesinin gebelik oranıüzerine etkisinin araştırılması amaçlandı. Çalışmada, toplam 40 adet primipar, Holstein- Friesian ırkı sağmal ineğe 0. günde10 μg GnRH ve 7. günde 500 μg PGF2α uygulanarak rastgele iki gruba ayrıldı. Prostaglandin uygulamasını takiben hayvanlara(n=21), 48. saatte (OVS 48), diğer gruptaki hayvanlara (n=19) ise 56. saatte 2. GnRH enjeksiyonu yapıldı (OVS 56).Protokolleri takiben 16. saatte suni tohumlama uygulandı. Serum progesteron (P4) değerlendirilmesi amacıyla, PGF2αnınuygulandığı 7. günde ve tohumlamalar sırasında kan örnekleri alındı. Gebelik tanısı, suni tohumlamadan sonraki 40- 45.günde transrektal ultrasonografi ile tespit edildi. Gruplar arasındaki 7. gün ve tohumlama anı ortalama serum P4değerlerinin değişimi incelendiğinde, (OVS 48: sırasıyla 11.09 ± 7.60 ve 0.89 ± 1.31 ng/ml olmasına karşın, OVS 56: 6.00 ±5.98 ve 1.10 ± 1.79 ng/ml) istatistiksel olarak önemli bir farklılık belirlenmedi (P>0.05). Çalışmada, farklı Ovsynchprogramları sonucunda elde edilen gebelik oranı OVS 48 grubunda % 66.66, OVS 56 grubunda ise % 47.36 olarak belirlendi.Ancak, gruplar arasındaki farklılığın istatistiksel olarak önemli olmadığı gözlendi (P>0.05). Sonuç olarak, sütçü ineklerdeklasik Ovsynch protokolünde ikinci GnRH enjeksiyonunun 8 saat geciktirilmesinin (48 yerine 56. saatte) gebelik oranı üzerineherhangi bir etkisinin olmadığı kanısına varıldı

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Cite This Article

EMRE, B., KORKMAZ, Ö., ZONTURLU, A. K. (2022). The Effect of Delaying the Administration of Second GnRH in the Ovsynch Protocol on Pregnancy Rate in Dairy Cows. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, Cilt: 9 - Sayı: 3, 187–193. https://ajindex.com/articles/455929

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References (2)

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