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Journals / Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi / 2018 / Cilt: 18 - Sayı: 3

In Vitro Investigation of the Anticancer Activity of Friedelin in Glioblastoma Multiforme

Bugrahan EMSEN
Tubanur ENGİN
Hasan TURKEZ
Pages
763–773
Publication Date
2018-12-30
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Consequently, the results obtained from the present study proved the potential of natural product with an antioxidant capacity as a source for anticancer compound against GBM.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:There are different herbal methods used for support in many cancer diseases.
📊
Key Findings & Data:It was used human U87MG-GBM cancer cell lines and primary rat cerebral cortex (PRCC) non-cancerous cells isolated from Sprague-Dawley rats in order to determine side effect level of FRI.
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Conclusion & Impact:Consequently, the results obtained from the present study proved the potential of natural product with an antioxidant capacity as a source for anticancer compound against GBM.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Cytotoxicity · Genotoxicity · Total Antioxidant Capacity

Özet

There are different herbal methods used for support in many cancer diseases. Lichens are important organisms containing unique herbal compounds and it is known that they have different anticancer activities. Starting from these features, the present study was aimed to investigate anticancer activity of friedelin (FRI), a lichen compound against glioblastoma multiforme (GBM) showed dangerous malignant properties within brain cancer species. It was used human U87MG-GBM cancer cell lines and primary rat cerebral cortex (PRCC) non-cancerous cells isolated from Sprague-Dawley rats in order to determine side effect level of FRI. In the experiments, cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) tests), antioxidant (via total antioxidant capacity (TAC) test), pro-oxidant (via total oxidative stress (TOS) test) and genotoxic (via 8-hydroxy-2′-deoxyguanosine (8-OH-dG) test) activities of different concentrations of FRI were tested. As a result of the study, MTT assay revealed that FRI showed higher cytotoxic activity on U87MG cells compared to PRCC cells (median inhibitory concentration (IC50): 46.38 and 1271.77 mg/L, respectively). Based on U87MG cells, it was determined a significant positive correlation between LDH and TOS activities. High positive correlation between TAC and cell viability on healthy PRCC cells exhibited antioxidant capacity of FRI. Consequently, the results obtained from the present study proved the potential of natural product with an antioxidant capacity as a source for anticancer compound against GBM.

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Cite This Article

EMSEN, B., ENGİN, T., TURKEZ, H. (2018). In Vitro Investigation of the Anticancer Activity of Friedelin in Glioblastoma Multiforme. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, Cilt: 18 - Sayı: 3, 763–773. https://ajindex.com/articles/451480

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References (11)

  1. Abuiraq, L., Kanan, G., Wedyan, M. and El-Oqlah, A., 2015. Efficacy of extracts of some lichens for potential antibacterial activity. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6, 318–331.
  2. Alpsoy, L., Aslan, A., Kotan, E., Agar, G. and Anar, M., 2011. Protective role of two lichens in human lymphocytes in vitro. Fresenius Environmental Bulletin, 20, 1661–1666.
  3. Ari, F., Celikler, S., Oran, S., Balikci, N., Ozturk, S., Ozel, M.Z., Ozyurt, D. and Ulukaya, E., 2014. Genotoxic, cytotoxic, and apoptotic effects of Hypogymnia physodes (L.) Nyl. on breast cancer cells. Environmental Toxicology, 29, 804–813.
  4. Bai, L., Bao, H.Y. and Bau, T., 2014. Isolation and identification of a new benzofuranone derivative from Usnea longissima. Natural Product Research, 28, 534–538. Bézivin, C., Tomasi, S., Lohézic-Le Dévéhat, F. and Boustie, J., 2003. Cytotoxic activity of some lichen extracts on murine and human cancer cell lines. Phytomedicine, 10, 499–503. Bézivin, C., Tomasi, S., Rouaud, I., Delcros, J.G. and Boustie, J., 2004. Cytotoxic activity of compounds from the lichen: Cladonia convoluta. Planta Medica, 70, 874–877.
  5. Bosset, J.-F., Collette, L., Calais, G., Mineur, L., Maingon, P., Radosevic-Jelic, L., Daban, A., Bardet, E., Beny, A., Ollier, J.-C. and EORTC Radiotherapy Group Trial 22921, 2006. Chemotherapy with preoperative radiotherapy in rectal cancer. The New England Journal of Medicine, 355, 1114–1123.
  6. Boustie, J. and Grube, M., 2005. Lichens-A promising source of bioactive secondary metabolites. Plant Genetic Resources, 3, 273–287.
  7. Brisdelli, F., Perilli, M., Sellitri, D., Piovano, M., Garbarino, J.A., Nicoletti, M., Bozzi, A., Amicosante, G. and Celenza, G., 2013. Cytotoxic activity and antioxidant capacity of purified lichen metabolites: an in vitro study. Phytotherapy Research, 27, 431–437.
  8. Brodo, I.M., Sharnoff, S.D. and Sharnoff, S., 2001. About the Lichens, in: Lichens of North America. Yale University Press, New Haven & London, 3–113.
  9. Clarke, J., Penas, C., Pastori, C., Komotar, R.J., Bregy, A., Shah, A.H., Wahlestedt, C. and Ayad, N.G., 2013. Epigenetic pathways and glioblastoma treatment. Epigenetics, 8, 785–795.
  10. Çelikler Kasımoğulları, S., Oran, S., Ari, F., Ulukaya, E., Aztopal, N., Sarimahmut, M. and Öztürk, Ş., 2014. Genotoxic, cytotoxic, and apoptotic effects of crude extract of Usnea filipendula Stirt. in vitro. Turkish Journal of Biology, 38, 940–947.
  11. Dogan, M., Emsen, B., Aasim, M. and Yildirim, E., 2017. Ceratophyllum