SavedAdd your journal

Journals / Eurasian Journal of Biological and Chemical Sciences / 2018 / Cilt: 1 - Sayı: 2

Anti-cytotoxic-genotoxic influences of in vitro propagated Bacopa monnieri L. Pennell in cultured human lymphocytes

Muhammet DOGAN
Bugrahan EMSEN
Pages
48–53
Publication Date
2018-12-20
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Overall, obtained data indicated that B. monnieri is a plant that contains natural products that are not causative of cytotoxic and genotoxic damage on human lymphocytes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Pennell is an important medicinal plant with antioxidant and neuroprotective role.
📊
Key Findings & Data:The shoot tip explants of B. monnneri were cultured in Murashige and Skoog (MS) nutrient medium containing 1.0 mg/L 6-Benzylaminopurine (BAP).
💡
Conclusion & Impact:Overall, obtained data indicated that B. monnieri is a plant that contains natural products that are not causative of cytotoxic and genotoxic damage on human lymphocytes.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Cytotoxicity · Genotoxicity · Shoot Regeneration

Abstract

Bacopa monnieri L. Pennell is an important medicinal plant with antioxidant and neuroprotective role. It acts as an efficient free radical scavenger and has the potential to serve as a phytoremedy in the disease conditions involving reactive oxygen species (ROS) and oxidative stress. In addition, the protective role of B. monnieri on tissue antioxidant defense system and lipid peroxidative status in diabetic rats has been reported. B. monnneri used in the experiment was propagated by plant tissue culture techniques. The shoot tip explants of B. monnneri were cultured in Murashige and Skoog (MS) nutrient medium containing 1.0 mg/L 6-Benzylaminopurine (BAP). Genotoxic effects of aqueous extract obtained from B. monnieri in cultured human lymphocytes were determined by chromosome aberration (CA) test. In addition, cytotoxic activities of the extract on the cells were revealed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis. Different concentrations (6.25-200 mg/L) of the extract were used in the treatments. It was detected that cytotoxicty was correlated with concentration. Cell viability decreased to 69.64% in the lymphocytes exposed to maximum concentration of the extract. Based on CA/cell test, the results were statistically (p > 0.05) indifferent from negative control treatment. Overall, obtained data indicated that B. monnieri is a plant that contains natural products that are not causative of cytotoxic and genotoxic damage on human lymphocytes.

🔖

Cite This Article

DOGAN, M., EMSEN, B. (2018). Anti-cytotoxic-genotoxic influences of in vitro propagated Bacopa monnieri L. Pennell in cultured human lymphocytes. Eurasian Journal of Biological and Chemical Sciences, Cilt: 1 - Sayı: 2, 48–53. https://ajindex.com/articles/756032

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Amoo SO, Aremu AO, Staden JV 2012. In vitro plant regeneration, secondary metabolite production and antioxidant activity of micropropagated Aloe arborescens Mill. Plant Cell Tissue Organ Cult 111: 345-358.
  2. Ari F, Ulukaya E, Oran S, Celikler S, Ozturk S, Ozel MZ 2015. Promising anticancer activity of a lichen, Parmelia sulcata Taylor, against breast cancer cell lines and genotoxic effect on human lymphocytes. Cytotechnology 67: 531–543.
  3. Aydin E, Turkez H 2011a. Effects of lichenic extracts (Bryoria capillaris, Peltigera rufescens and Xanthoria elegans) on human blood cells: A cytogenetic and biochemical study. Fresenius Environ Bull 20: 2992–2998.
  4. Aydin E, Turkez H 2011b. Antioxidant and genotoxicity screening of aqueous extracts of four lichens collected from North East Anatolia. Fresenius Environ Bull 20: 2085–2091.
  5. Bairu MW, Amoo SO, Staden JV 2011. Comparative phytochemical analysis of wild and in vitro-derived greenhouse-grown tubers, in vitro shoots and callus-like basal tissues of Harpagophytum procumbens. S Afr J Bot 77: 479-484.
  6. Baskaran P, Moyo M, Staden JV 2014. In vitro plant regeneration, phenolic compound production and pharmacological activities of Coleonema pulchellum. S Afr J Bot 90: 74-79.
  7. Bayram S, Ecem Bayram N, Gerçek YC, Öz GC, Sorkun K 2016. Anticytotoxic and antimutagenic effects of propolis on human lymphocytes in vitro. Mellifera 16: 38–46.
  8. Bhattacharya SK, Bhattacharya A, Kumar A, Ghosal S 2000. Antioxidant activity of Bacopa monnieri in rat frontal cortex, striatum and hippocampus. Phytother Res 14: 174-179. Bourrain L 2018. In vitro propagation of Actinidia melanandra Franch. and Actinidia rubricaulis Dunn. from shoot tip explants. N Z J Crop Hortic Sci 46(2): 162-173.
  9. Danova K, Cellárová E, Macková A, Daxnerová Z, Kapchina-Toteva V 2010. In vitro culture of Hypericum rumeliacum Boiss and production of phenolics and flavonoids. In Vitro Cell Dev Biol 46: 422-429.
  10. Dogan M, Karatas M, Aasim M 2015. An efficient in vitro plantlet regeneration of Ceratophyllum demersum L., an important medicinal aquatic plant. Fresen Environ Bull 24(10b): 3499-3504. Dogan M 2017. Multiple shoot regeneration from shoot tip and nodal explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Ant J Bot 1(1,2): 4-8. Dogan M 2018a.
  11. Tıbbi bitki Lysimachia nummularia L.'nın boğum eksplantlarından in vitro mikroçoğaltımı. KSÜ Tarım ve Doğa Dergisi, 21(6): 875-881. Dogan M 2018b. Limnophila aromatica (Lamk.) Merr.'nın boğum ve boğum arası eksplantlarından i