Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2003 / Cilt: 50 - Sayı: 1

Liver and serum vitamin A-carotene levels in broilers following dietary aflatoxin exposure

Diyetle aflatoksin alan broylerlerde karaciğer ve serum vitamin A-karotin düzeyleri

Pages
1–6
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a most effective dose, 1000 ppb AF intake with feed for 30-45 days were significantly lowered serum and liver vitamin A levels.

📌 3 Key Takeaways

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Objective & Hypothesis:Serum and liver vitamin A-carotene levels were investigated in broilers exposed to 50, 100, 500 and 1000 ppb of aflatoxin (AF) during 45 days.
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Key Findings & Data:Totally, 75 broilers were divided into 5 groups containing 15 broilers in each group.
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Conclusion & Impact:As a most effective dose, 1000 ppb AF intake with feed for 30-45 days were significantly lowered serum and liver vitamin A levels.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Serum and liver vitamin A-carotene levels were investigated in broilers exposed to 50, 100, 500 and 1000 ppb of aflatoxin (AF) during 45 days. Totally, 75 broilers were divided into 5 groups containing 15 broilers in each group. Control group was fed with normal feed. Trial groups were fed with AF added feed. Ad libitum water was given to broilers in all groups. On the 15" 30lh and 45lh days of the study, five animals from trial and control groups were slaughtered to abtain the blood and liver tissues. Vitamin A and carotene levels were measured spectrophotometrically on these tissues. Statistical significance of average means ac-cording to the time and dose of aflatoxin intake and differences between groups were evaluated using nonparametric varians analysis and Mann-Whitney-U test. Vitamin A levels were initially decreased following the intake of feed added AF. Following this decrease vitamin A levels were increased depend on the doses of AF. Moreover, vitamin A levels in broilers consumed 1000 ppb AF for 30 days were significantly decreased (14.98±1.86 p.g/g) and this effect continued up to day 45. Carotene levels of liver were not effected with the doses of AF. Howewer, it was temporally lowered on the day 30. Vitamin A and carotene levels were also significantly dec-reased with feed intake including 1000 ppb AF. The most lowered levels (14.72±2.75 and 34.34±8.56 jig/dl, respectively) were ob-served in this group. As a result, AF intake with feed had a different effect on vitamin A metabolism of broilers depend on the dose and intake time. As a most effective dose, 1000 ppb AF intake with feed for 30-45 days were significantly lowered serum and liver vitamin A levels.

Özet

Çalışmada, yemle 15, 30 ve 45 gün süreyle 50, 100, 500 ve 1000 ppb miktarda aflatoksin (AF) verilmesinin broylerlerde serum ve karaciğer vitamin A ve karotin düzeyleri üzerine etkisi incelenmiştir. Her grupta 15 hayvan olacak şekilde 75 hayvandan 5 grup oluşturulmuş ve gruplardan birine normal yem diğerlerine AF katılmış yem ile birlikte ad libidum su verilmiştir, Çalışmanın 15, 30 ve 45. günlerinde deneme ve kontrol grubundaki hayvanlardan 5'er adet kesilerek kan ve karaciğer örnekleri alınmış, vitamin A ve karotin düzeyleri UV-spektrofotometrik olarak ölçülmüştür. Aflatoksinin miktarına ve alım süresine göre elde edilen ortalama değerler ile gruplararası farklılıkların istatistik önemlilikleri parametrik olmayan varyans analizi ve Mann-Whitney-U testi ile de-ğerlendirilmiştir. Yemle 45 gün süreyle 50 ppb AF verilmesinin broylerlerde karaciğer vitamin A değerini yükselttiği, 30 gün süreyle 1000 ppb verilmesinin ise karaciğer vitamin A miktarını önemli derecede düşürdüğü ve bu etkinin 45. günde de sürdüğü; karaciğer karotin değerlerinin ise AF dozlarından etkilenmediği ancak, maruz kalma süresine bağlı olarak 30. günde geçici olarak dü.şüş kay-dettiği gözlenmiştir. Yem ile 45 gün 1000 ppb AF verilmesinin serum vitamin A ve karotin değerlerinde de önemli düşüşe neden ol-duğu (en düşük değerler 14.72±2.75 ve 34.34+8.56 u.g/dl bu grupta) saptanmıştır. Sonuç olarak, yemle AF verilmesinin broyler ka-raciğer vitamin A metabolizmasını doza ve maruz kalma süresine bağlı olarak farklı şekilde etkilediği ve yemde 1000 ppb AF'nin broylerlerde vitamin A için etkili miktar olduğu; bu miktarda AF'nin 30-45 gün süreyle verilmesinin karaciğer ve serum vitamin A değerlerinde önemli düşüşlere yol açtığı kanısına varılmıştır.

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Cite This Article

KONTAŞ, T., ALTINTAŞ, A., ERASLAN, G., BİLGİLİ, A. (2022). Liver and serum vitamin A-carotene levels in broilers following dietary aflatoxin exposure. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 50 - Sayı: 1, 1–6. https://ajindex.com/articles/445295

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